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Eberhard-Karls-Universität Tübingen

1 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Organotypic Retinal Explant Cultures from Macaque Monkey
Wenrong Xu 1, Yujie Dong 1, Yan Li 1, Zhulin Hu 1, François Paquet-Durand 2, Kangwei Jiao 1
1Yunnan Eye Institute & Key Laboratory of Yunnan Province, Yunnan Eye Disease Clinical Medical Center, Affiliated Hospital of Yunnan University, Yunnan University, 2Institute for Ophthalmic Research, Eberhard-Karls-Universität Tübingen

Retinal explants obtained from wild-type macaques were cultured in vitro. Retinal degeneration and the cGMP-PKG signaling pathway was induced using the PDE6 inhibitor zaprinast. cGMP accumulation in the explants at different zaprinast concentrations was verified using immunofluorescence.

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