Isolation of Regenerating Hepatocytes after Partial Hepatectomy in MiceEva Breuer 1, Bostjan Humar 1
1Swiss HPB Laboratory, Department of Visceral & Transplant Surgery, University Hospital Zurich (USZ)
Lipid-laden hepatocytes are inherent to liver regeneration but are usually lost upon density-gradient centrifugation. Here, we present an optimized cell isolation protocol that retains steatotic hepatocytes, yielding representative populations of regenerating hepatocytes after partial hepatectomy in mice.
