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Fraunhofer Institute for Cell Therapy and Immunology

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain

Claudia Pösel^*1, Karoline Möller^*1, Johannes Boltze^1,2, Daniel-Christoph Wagner¹, Gesa Weise^1,3

¹Ischemia Research Unit, Fraunhofer Institute for Cell Therapy and Immunology, ²Fraunhofer Research Institution for Marine Biotechnology, University of Lubeck, ³Department of Neurology, University of Leipzig

Inflammation plays a central role in the pathogenesis of ischemic stroke. Increasing evidence suggests that it acts as a double-edged sword which exacerbates early brain injury, but also contributes to later repair. This protocol describes the isolation of immune cells from the ischemic brain and their subsequent flow cytometric phenotyping.

Bioengineering

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Jörg Schnauß^*1,2, Martin Glaser^*1,2, Jessica S. Lorenz¹, Carsten Schuldt^1,2, Christin Möser¹, Martin Sajfutdinow¹, Tina Händler^1,2, Josef A. Käs², David M. Smith¹

¹Fraunhofer Institute for Cell Therapy and Immunology, ²Institute of Experimental Physics I, Universität Leipzig

Semiflexible polymers display unique mechanical properties that are extensively applied by living systems. However, systematic studies on biopolymers are limited since properties such as polymer rigidity are inaccessible. This manuscript describes how this limitation is circumvented by programmable DNA nanotubes, enabling experimental studies on the impact of filament rigidity.

Effective Lysis of Cyanobacterial and Green Algal Single Cells for Whole Genome Amplification in Microfluidics

Yuguang Liu¹, Dirk Schulze-Makuch², Jean-Pierre de Vera³, Charles Cockell⁴, Thomas Leya⁵, Mickael Baque³, Marina Walther-Antonio^1,6

¹Department of Surgery, Division of Surgical Research, Mayo Clinic, ²Astrobiology Group, Center of Astronomy and Astrophysics, Technical University, ³Management and Infrastructure, Astrobiological Laboratories, German Aerospace Center (DLR), Institute of Planetary Research, ⁴School of Physics and Astronomy, University of Edinburgh, ⁵Branch Bioanalytics and Bioprocesses (IZI-BB), Extremophile Research & Biobank CCCryo, Fraunhofer Institute for Cell Therapy and Immunology, ⁶Department of Obstetrics and Gynecology, Mayo Clinic

Here we present a protocol to lyse cyanobacteria and green algae single cells that allows for subsequent single-cell whole genome amplification in a microfluidic platform with a 100% success rate.