Polarimeter

概要

Source: Vy M. Dong and Diane Le, Department of Chemistry, University of California, Irvine, CA

This experiment will demonstrate the use of a polarimeter, which is an instrument used to determine the optical rotation of a sample. Optical rotation is the degree to which a sample will rotate polarized light. Optically active samples will rotate the plane of light clockwise (dextrorotatory), designated as d or (+), or counterclockwise (levorotatory), designated as l or (−).

手順

1. Preparing the Polarimeter

  1. Turn on instrument and let it warm up for 10 min.
  2. Make sure instrument is set to "optical rotation" mode.
  3. Prepare a blank sample in the polarimeter cell (1.5 mL total sample volume, 1 dm in length) containing only CHCl3. Make sure there are no air bubbles present.
  4. Place the blank cell in the holder and press "zero."

2. Preparation of Analyte Sample

  1. Prepare a stock solution of 10

Log in or to access full content. Learn more about your institution’s access to JoVE content here

結果

Representative results for the measurement and calculation of specific rotation for Procedures 1-4.

Procedure Step Reading on polarimeter
1.4 0.000
3.2 +0.563
4.1 [α]25D = +77° (c 0.73, CHCl3)

Log in or to access full content. Learn more about your institution’s access to JoVE content here

申請書と概要

In this experiment, we have demonstrated the principles behind the polarimeter and how to measure and calculate the specific rotation of an optically active compound.

The polarimeter is an important instrument in the fine-chemical and pharmaceutical industries to assess the identity, purity, and quality of a compound. It is specifically used for the measurement of optical rotation of chiral compounds, which can be used to distinguish the identity of two enantiomers by confirming whether it is

Log in or to access full content. Learn more about your institution’s access to JoVE content here

タグ
PolarimeterPurity AssessmentChemical PropertiesEnantiomersMirror image VariantsBiological ActivitiesPharmaceuticalsDrug ThalidomidePrinciples Of PolarimetrySetup And OperationApplicationsOrganic CompoundsStereocentersChiral CompoundsMirror image IsomersEnantiomersPhysical PropertiesOptics

スキップ先...

0:00

Overview

0:49

Principles of Polarimetry

3:51

Initialization of the Polarimeter

4:24

Calibration and Measurement

5:23

Applications

6:52

Summary

このコレクションのビデオ:

article

Now Playing

Polarimeter

Organic Chemistry II

99.6K 閲覧数

article

クリーニングのガラス製品

Organic Chemistry II

122.8K 閲覧数

article

求核置換反応

Organic Chemistry II

99.0K 閲覧数

article

還元剤

Organic Chemistry II

42.6K 閲覧数

article

グリニャール反応

Organic Chemistry II

148.5K 閲覧数

article

n- ブチル リチウムの滴定

Organic Chemistry II

47.5K 閲覧数

article

ディーン ・ スターク ・ トラップ

Organic Chemistry II

99.5K 閲覧数

article

アルケンのオゾン分解

Organic Chemistry II

66.6K 閲覧数

article

有機触媒反応

Organic Chemistry II

16.5K 閲覧数

article

パラジウム触媒クロス カップリング

Organic Chemistry II

33.7K 閲覧数

article

固相合成

Organic Chemistry II

40.6K 閲覧数

article

水素化

Organic Chemistry II

48.7K 閲覧数

article

重合

Organic Chemistry II

93.0K 閲覧数

article

融点

Organic Chemistry II

149.0K 閲覧数

article

赤外分光法

Organic Chemistry II

213.4K 閲覧数

JoVE Logo

個人情報保護方針

利用規約

一般データ保護規則

研究

教育

JoVEについて

Copyright © 2023 MyJoVE Corporation. All rights reserved