Source: Vy M. Dong and Diane Le, Department of Chemistry, University of California, Irvine, CA
This experiment will demonstrate the use of a polarimeter, which is an instrument used to determine the optical rotation of a sample. Optical rotation is the degree to which a sample will rotate polarized light. Optically active samples will rotate the plane of light clockwise (dextrorotatory), designated as d or (+), or counterclockwise (levorotatory), designated as l or (−).
1. Preparing the Polarimeter
2. Preparation of Analyte Sample
Representative results for the measurement and calculation of specific rotation for Procedures 1-4.
Procedure Step | Reading on polarimeter |
1.4 | 0.000 |
3.2 | +0.563 |
4.1 | [α]25D = +77° (c 0.73, CHCl3)
申請書と概要 In this experiment, we have demonstrated the principles behind the polarimeter and how to measure and calculate the specific rotation of an optically active compound. The polarimeter is an important instrument in the fine-chemical and pharmaceutical industries to assess the identity, purity, and quality of a compound. It is specifically used for the measurement of optical rotation of chiral compounds, which can be used to distinguish the identity of two enantiomers by confirming whether it is タグ スキップ先... このコレクションのビデオ: ![]() Now Playing PolarimeterOrganic Chemistry II 99.6K 閲覧数 ![]() クリーニングのガラス製品Organic Chemistry II 122.8K 閲覧数 ![]() 求核置換反応Organic Chemistry II 99.0K 閲覧数 ![]() 還元剤Organic Chemistry II 42.6K 閲覧数 ![]() グリニャール反応Organic Chemistry II 148.5K 閲覧数 ![]() n- ブチル リチウムの滴定Organic Chemistry II 47.5K 閲覧数 ![]() ディーン ・ スターク ・ トラップOrganic Chemistry II 99.5K 閲覧数 ![]() アルケンのオゾン分解Organic Chemistry II 66.6K 閲覧数 ![]() 有機触媒反応Organic Chemistry II 16.5K 閲覧数 ![]() パラジウム触媒クロス カップリングOrganic Chemistry II 33.7K 閲覧数 ![]() 固相合成Organic Chemistry II 40.6K 閲覧数 ![]() 水素化Organic Chemistry II 48.7K 閲覧数 ![]() 重合Organic Chemistry II 93.0K 閲覧数 ![]() 融点Organic Chemistry II 149.0K 閲覧数 ![]() 赤外分光法Organic Chemistry II 213.4K 閲覧数 Copyright © 2023 MyJoVE Corporation. All rights reserved |