DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation

1:16

Introduction

1:49

Plasmid Preparation for Electroporation

2:46

Animal Preparation for Electroporation

6:12

Electroporation and Transfection

8:31

Assaying In-Vivo Transfection by Electroporation

8:36

Transfection Efficiency

9:47

Subcellular Protein Localization

12:31

Expression and Targeting of the a-sub-unit of the Dihydropyridine Receptor (DHPR-a1s)

13:56

Functional Evaluation of the Expression of EYFP-CIC1

15:30

Conclusion