The video discusses a surgical procedure for implanting bone screws in a mouse's skull. It covers preparation steps, locating screw placement, scoring the skull, and applying liquid cyanoacrylate to secure the screws while avoiding damage to the underlying dura, ensuring the success of the micro drive implant.

surgical implantation of the micro-drive on the mouse skull

若年マウスの日常神経活動を記録するためのマイクロドライブインプラント

The brain undergoes large-scale changes during the critical developmental windows of childhood and adolescence1,2,3. Many neurologic and psychiatric diseases, including autism and schizophrenia, first manifest behaviorally and biologically during this period of juvenile and adolescent brain development4,5,6. While much is known regarding the cellular, synaptic, and genetic changes that occur across early development, comparatively little is known regarding how circuit- or network-level processes change throughout this time window. Importantly, circuit-level brain function, which ultimately underlies complex behaviors, memory, and cognition, is a non-predictable, emergent property of cellular and synaptic function7,8,9,10. Thus, to fully understand network-level brain function, it is necessary to directly study neural activity at the level of an intact neural circuit. In addition, to identify how brain activity is altered throughout the progression of neuropsychiatric disorders, it is critical to examine network activity in a valid disease model during the specific temporal window when the behavioral phenotypes of the disease manifest and to track the observed changes as they persist into adulthood.
One of the most common and powerful scientific model organisms is the mouse, with large numbers of unique genetic strains that model neurodevelopmental disorders with age-dependent onset of the behavioral and/or mnemonic phenotypes11,12,13,14,15,16,17,18,19,20,21. While it is challenging to correlate precise developmental time points between the brains of humans and mice, morphological and behavioral comparisons indicate that p20-p21 mice represent the human ages of 2-3 years old, and p25-p35 mice represent the human ages of 11-14 years old, with mice likely reaching the developmental equivalent of a human 20 year old adult by p603,22. Thus, to better understand how the juvenile brain develops and to identify how the neural networks of the brain become dysfunctional in diseases like autism or schizophrenia, it would be ideal to directly monitor brain activity in vivo in mice across the ages of 20 days to 60 days old.
However, a fundamental challenge in monitoring brain activity across early development in mice is the small size and relative weakness of juvenile mice. The chronic implantation of electrodes, which is necessary for longitudinal studies of brain development, typically requires large, bulky housing to protect the fine electrode wires and interface boards23,24, and the implants must be firmly attached to the mouse skull, which is thinner and less rigid in young mice due to reduced ossification. Thus, virtually all studies of in vivo rodent physiology have been performed in adult subjects due to their relative size, strength, and skull thickness. To date, most studies exploring in vivo juvenile rodent brain physiology have been performed in wild-type juvenile rats, which necessarily limits the ability to experimentally monitor juvenile brain function in a freely behaving model of a human disorder25,26,27,28,29,30.
This manuscript describes novel implant housing, a surgical implantation procedure, and a post-surgery recovery strategy to chronically study the long-term (up to 4 or more weeks) in vivo brain function of juvenile mice across a developmentally critical time window (p20 to p60 and beyond). The implantation procedure allows for the reliable, permanent affixation of the electrodes to the skulls of juvenile mice. Furthermore, the micro-drive design is lightweight, as this micro-drive weighs ~4-6 g when fully assembled, and due to the minimal counterbalancing required to offset the weight of the implant, it does not impact the behavioral performance of juvenile mice during typical behavioral paradigms.

著者スポットライト:若年マウスの慢性四極管記録のための軽量ドライブインプラント  

幼若マウスにおける慢性四尾行記録のための軽量駆動インプラント

Watch this Scientific Journal Video about Surgical Implantation of the Micro-Drive on the Mouse Skull at JoVE.com

Surgical Implantation of the Micro-Drive on the Mouse Skull  

マウス頭蓋骨へのマイクロドライブの外科的移植

マウスを手術の準備をし、頭蓋骨を露出させた後、骨が最も厚く、骨ネジがマイクロドライブインプラントから十分に離れている頭蓋骨の極端な外側、吻側、または尾側の部分に骨ネジを配置します。メスの刃またはドリルビットを使用して、骨ねじ穴の位置の近くで頭蓋骨にスコアを付け、液体シアノアクリレートが結合するための粗い表面を提供します。次に、下にある硬膜を突き刺さないように注意しながら、各骨ネジを所定の位置にねじ込みます。滅菌済みの30ゲージ針を使用して、各骨ネジの周りに液体シアノアクリレートを塗布し、骨ネジが取り付けられている頭蓋骨を厚くします。シアノアクリレートが記録部位の上の露出した硬膜に入らないようにしてください。

surgical-implantation-of-the-micro-drive-on-the-mouse-skull

Research

JoVE Journal

Neuroscience

Surgical Implantation of the Micro-Drive on the Mouse Skull

547 ビュー. マウスを手術の準備をし、頭蓋骨を露出させた後、骨が最も厚く、骨ネジがマイクロドライブインプラントから十分に離れている頭蓋骨の極端な外側、吻側、または尾側の部分に骨ネジを配置します。メスの刃またはドリルビットを使用して、骨ねじ穴の位置の近くで頭蓋骨にスコアを付け、液体シアノアクリレートが結合するための粗い表面を提供します。次に、下?...

ビデオ: Surgical Implantation of the Micro-Drive on the Mouse Skull  

A Lightweight Drive Implant for Chronic Tetrode Recordings in Juvenile Mice

In vivo electrophysiology provides unparalleled insight into the sub-second-level circuit dynamics of the intact brain and represents a method of particular importance for studying mouse models of human neuropsychiatric disorders. However, such methods often require large cranial implants, which cannot be used in mice at early developmental time points. As such, virtually no studies of in vivo physiology have been performed in freely behaving infant or juvenile mice, despite the fact that a better understanding of neurological development in this critical window would likely provide unique insights into age-dependent developmental disorders such as autism or schizophrenia. Here, a micro-drive design, surgical implantation procedure, and post-surgery recovery strategy are described that allow for chronic field and single-unit recordings from multiple brain regions simultaneously in mice as they age from postnatal day 20 (p20) to postnatal day 60 (p60) and beyond, a time window roughly corresponding to the human ages of 2 years old through to adulthood. The number of recording electrodes and final recording sites can be easily modified and expanded, thus allowing flexible experimental control of the in vivo monitoring of behavior- or disease-relevant brain regions across development.

Here, we describe a micro-drive design, surgical implantation procedure, and post-surgery recovery strategy that allow for chronic field and single-unit recordings from multiple brain regions simultaneously in juvenile and adolescent mice across a critical developmental window from postnatal day 20 (p20) to postnatal day 60 (p60) and beyond.