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Isolating and Infecting Primary Cortical Mixed Glia with Prions

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06:28 min

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August 11th, 2023

DOI :

10.3791/200271-v

August 11th, 2023

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Arielle J. D. Hay¹^,², Katriana A. Popichak¹^,², Mark D. Zabel¹^,², Julie A. Moreno¹^,³

¹Prion Research Center, Colorado State University, ²Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, ³Department of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University

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Keywords Primary Cortical Mixed Glia

Prion Infection

Brain Tissue Isolation

Tissue Dissociation

Brain Homogenate

UV Light Exposure

Adipose Tissue Isolation

Enzymatic Digestion

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