JoVE Journal

Neuroscience

This content is Open Access.

Monitoring Cleaved Caspase-3 Activity and Apoptosis of Immortalized Oligodendroglial Cells using Live-cell Imaging and Cleaveable Fluorogenic-dye Substrates Following Potassium-induced Membrane Depolarization

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10:45 min

January 13th, 2012

January 13th, 2012

15,816 Views

0:05

Title

1:22

Culture and Transfection of Oligodendroglial Cells

4:13

Preparing Cells for Live-cell Image (LCI)

6:21

Treatment of Cells with Apoptosis Inducers and NucView 488 Caspase-3 Substrate

7:20

Live-cell Imaging

8:27

Cleaved Caspase-3 Actitity after Poassium-induced Membrane Depolarization

10:10

Conclusion

Live-cell imaging of caspase-3 mediated apoptosis in immortalized N19-oligodendrocyte cell cultures using the NucView 488 caspase-3 substrate. This technique is applicable for programmed cell death assays in real-time in a variety of cell types and tissues.

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