JoVE Journal

Bioengineering

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Measuring Left Ventricular Pressure in Late Embryonic and Neonatal Mice

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08:15 min

February 23rd, 2012

February 23rd, 2012

13,798 Views

0:05

Title

1:13

Ultrasound and Pressure System Overview and Set-up

3:23

Mouse Preparation

4:55

Ventricular Pressure Measurement with Ultrasound

7:10

Pulsatile Pressure and Heart-rate Measurements

7:51

Conclusion

Measuring left ventricular pressure (LV) in embryonic and neonatal mice is described. Pressure is measured by inserting a needle connected to a fluid-filled transducer into the LV under ultrasound guidance. Care must be taken to maintain normal cardiac function during the experimental protocol.

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