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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

8.9K Views

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10:06 min

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April 26th, 2017

DOI :

10.3791/54967-v

April 26th, 2017

•

8,866 Views

Huan-Huan Wei*¹, Yuanlong Liu*¹, Yang Wang², Qianyun Lu¹, Xuerong Yang¹, Jiefu Li¹, Zefeng Wang¹

¹Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences (SIBS), ²Institute of Cancer Stem Cell, Second Affiliated Hospital, Cancer Center, Dalian Medical University

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文字起こし

要約

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Alternative Splicing

Artificial Splicing Factors

Splicing Reporter Plasmids

Glycine rich Domain

RS effector Domain

PGL RS PUF Expression Vector

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