Name: intraductal delivery to the rabbit mammary gland
Uploaded: 2017-03-09T13:00:00.000+00:00
Duration: 6 min 30 s
Description: Watch this Scientific Journal Video about Intraductal Delivery to the Rabbit Mammary Gland at JoVE.com

The authors acknowledge support from a Translational Breast Cancer Research Grant (14-60-26-BROC to AB) from the Breast Cancer Research Foundation and the American Association for Cancer Research.

動物被験体を使用した手順は、テキサス大学オースティン校の施設内動物管理使用委員会によって承認されています。 1.術前の準備<ol><li>各ウサギの体重を記録します。すべての前臨床試験と同様に、潜在的な毒性を評価するために定期的に動物の体重を監視します。 </li><li>ウサギを麻酔する前に、30秒間500×gで、市販の超音波ゲルとスピンで50 mLのコニカルチューブを埋めます。遠心分離終了時のゲルで目に見える泡があってはなりません。 </li><li> 0.75ミリグラム/ kgの用量でアセプロマジン筋肉内には0.1mg / kgの用量でグリコピロレートを皮下に投与し、。バイタルサインや行動を監視しながら有効にする鎮静のために15〜20分待ちます。 注：グリコピロレートは、徐脈を防止し、呼吸器およびGI分泌を低下させる抗コリン薬です。アセプロマジンはpremedicatiとして機能鎮静剤であります麻酔のために。 </li><li>ケタミン35mgの/キロとキロのキシラジン皮下麻酔薬としては5mg /を管理します。しかし、オペレータは、より経験豊富になるとケタミンとの3mg / kgの15 mgの薬剤の投与量を減少させ、より迅速に管内の配信を達成することができるように/キシラジンの皮下キロ。これは麻酔時間と麻酔から回復する動物に要する時間を短縮します。 </li><li>チェックして、心拍数、SPO 2、温度、呼吸数、および粘膜の色ごとに15分を記録。両眼に眼の潤滑剤を塗布します。 注：のみ適切な訓練を受けており、それらの機関のIACUCによって承認された職員は、麻酔を管理または監視する必要があります。獣医の麻酔モニタの使用はバイタルサインを取得するのを助けることができると推奨されています。獣医の麻酔モニタは、しかし、手動で動物を15分ごとにチェックする必要性を置き換えるものではありません。 fは、製造元の指示を参照してください。または獣医学的監視の適切な使用。 </li><li>穏やかなつま先のピンチによって麻酔の開始を確認します。ウサギは、続行する前に非応答性であるべきです。 </li><li>慎重に鼠径乳首3番目と4番目のペアの周りの領域にウサギの尾側腹部を剃ります。 </li><li>削除髪の大部分では、剃った領域に店頭脱毛クリームを適用します。暖かい水で湿らせた湿ったペーパータオルを使用して、塗布後のクリーム10分を削除してください。 注：ウサギの皮膚が敏感な/非常に壊れやすいです。脱毛クリームは、せいぜい10分のために使用されるべきです。実際には、5分で「テスト・スポット」を試してみて、テストのみスポットがより長い期間が必要とされる示している場合に長く残すために安全です。 </li><li>注射部位を洗浄する際には、アルコールに浸したガーゼパッドで拭きます。 </li><li>再循環温水​​ブランケットと吸収パッドが並んでV字型の溝で、その背にウサギを置きます。 </li></ol> 2. Prを造影剤のeparation <ol><li>製造業者の説明書に従って、非標的化造影試薬を再構成します。混合する優しく上下にピペット。 注： - 再構成後6時間、このプロトコルで使用される造影剤は、4室温で安定です。静かに各検索の間にバイアルを揺すります。 注：必要な溶液の量は、注入されるダクトの数に依存します。ウサギは乳首あたり4腺管開口部、および液0.2 mLのを持っている大人のニュージーランドホワイトラビット（Oryctolaguscuniculus）の1乳管ツリーを満たすのに十分です。したがって、0.8mLの総容量は、一つ乳腺に送達することができます。 </li></ol> 3.管内配達<ol><li>注入されるべき適切な乳首の位置を確認します。動物がその背側に配置されたときに、それらが容易に可視化されているよう鼠径乳首の3 番目と4 番目のペアは、推奨されています。 </li><li> 0.9％滅菌生理食塩水の負荷を0.2mL22 G針で1 mLのルアーロックツベルクリン注射器。適切に生理食塩水を注射器になると22 G針を処分し、無菌の25ゲージの針に交換してください。ゆっくりガーゼパッド上の85％のイソプロピルアルコールで拭きます。 </li><li>ニードルアップや動物の体内に注射器の並列のベベルでは、乳頭の側に針のベベルを挿入し、ゆっくりと生理食塩水の0.1から0.2 mLのを注入します。これは、乳管開口部のより良い視覚化を可能にします。 </li><li>負荷1 mLのルアーロックツベルクリン注射器に注射液0.2 mLです。 </li><li>親指と人​​差し指で軽く乳首を持って、管内の注射のためにそれを配置するために少し持ち上げ、ウェアラブル眼ルーペは、乳管開口部を視覚化するのを助けることができます。 </li><li>乳首の上昇位置を維持しながら、慎重に25 G鈍先端の針を使用して、関心のダクトにカニューレを挿入。 </li><li>挿管後、軽く鈍-トンのハブのルアーロックシリンジをねじりますそれが所定の位置にロックされるまで、腹腔注射針。 </li><li>乳首を持ち上げて、急速にダクト内の流体を移動させることによって引き起こされる潜在的な損傷を最小限に抑えるために、ゆっくりとソリューションを注入。液を注入する際、時間がない時に抵抗があるはずです。 </li></ol> 4.超音波画像<ol><li>関心領域の皮膚に遠心分離、超音波ゲルのリベラルな量を適用します。これらは、画像品質を妥協するように、ゲル中に気泡がないことを確認してください。 </li><li> 6ミリメートルにイメージング深さを設定します。ゲルと接触して21 MHzのトランスデューサーを配置し、Bモードでの関心領域をスキャンします。乳管開口部とダクトを通して含む走査領域における造影剤を観察します。 注：これらの設定は、特定の光音響超音波機器で使用するために開発されてきました。詳細については、材料の表を参照してください。乳房を最適化する送信電力及び他の撮像システムの撮像深さを調整する必要があるかもしれません腺の可視化。 </li><li>ガーゼパッドを動物の皮膚からの超音波ゲルを削除します。 </li></ol> 5.術後ケア<ol><li>乳頭領域に対するまたは周囲の組織への外傷の兆候があってはならない、と乳首を囲む領域で膨潤が可能性が高い乳房脂肪パッド注射ではなく、成功した管内注射を示し：注射部位を観察します。 </li><li>胸骨の位置にウサギを置きます。必要に応じて、静脈内に辺縁耳静脈にヨヒンビンの0.2ミリグラム/キログラムを与えます。これは、キシラジンの効果を逆転し、動物が麻酔からより迅速に回復することができます。 </li><li>動物が応答、警告であり、胸骨位置を維持するまで、回復期間を通してウサギを15分ごとに監視します。 注：この手順は、組織の損傷や腫れが生じてはなりません。赤みや腫れが認められた場合、動物が警告とできるようになると、メロキシカムは0.1〜0.2mg / kgのPOの用量を投与経口薬を服用します。さらに指導のために金融機関の獣医のスタッフにお問い合わせください。 </li></ol>

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The authors have nothing to disclose.

ウサギの乳腺へ管内送達のこの方法は、超音波造影試薬および不可欠な染料および治療薬を含む多くの他の水溶液に使用することができます。以前の研究は、ホルモン17、18、19の管内送達を実証しました。げっ歯類モデル、核酸8の管内送達、化学療法剤6,7、及...

<html>治療薬の管内送達は、齧歯類モデルおよび早期臨床試験3、4、5、6、11、12で検討されています。私は勉強最近のフェーズは、管内カルボプラチンまたは浸潤癌2の治療のために乳房切除術を待っている女性では管内ペグ化リポソームドキソルビシンの安全性と実現可能性を実証しました。 管内送達のための以前のプロトコルは、マウスおよびラット乳腺6、7、8、9のために開発されています。研究目的、管内腫瘍細胞注射および癌遺伝子のレンチウイルスベクターの送達のためにも、げっ歯類モデルで実施されていますREF &quot;&gt; 13、14、15、16が、管内デリバリプロセスのインビボモデルにおける理想的な治療用化合物の新規なクラスの開発を可能にし、前臨床評価を容易にするはずである。げっ歯類とヒトとの間の解剖学的差異は、これらの変換を複雑にしています研究。 マウスとは異なり、各ダクトは別々の乳首で終了した、ヒト乳房は5〜9の独立性乳管システム、乳首で終わる独立した開口部を有する各構成されています。ウサギの乳腺は、4つの独立した腺管システム、単一乳首の4つのオリフィスのうちの1つを介してそれぞれ個別にアクセスを抱きます。ウサギモデルは、より密接に人体解剖学と一致し、より関連性の文脈で管内薬物送達の研究を可能にします。 ここでは、管内の配信を評価するために2つの手法を使用します。の同時投与生体色素が皮膚を通して可視化を可能にし、方法の簡単かつ迅速な確認を提供します。いくつかの用途のために、管のより高い解像度のマッピングが好ましい場合があります。我々は、非標的コントラスト試薬の管内配信を通じてここダクトの超音波イメージングのためのプロトコルを提示します。

<table><tbody><tr><td>MicroMarker non-targeted contrast reagent</td><td>VisualSonics</td><td>VS-11694</td><td></td></tr><tr><td>Luer Lock 1 mL Syringes</td><td>BD</td><td>309628</td><td></td></tr><tr><td>Glycopyrrolate 0.2 mg/mL</td><td>Wedgewood Compounding Pharmacy</td><td>GLYCOP-INJ013VC</td><td>6 month shelf life, supply may be limited.&amp;nbsp;</td></tr><tr><td>Atropine Sulfate 0.5 mg/mL</td><td>Animal Health International</td><td>15320764</td><td>If glycopyrrolate is unavailable. Not to be combined with glycopyrrolate.</td></tr><tr><td>Ketamine HCL 100 mg/mL</td><td>Animal Health International</td><td>21250699</td><td>&lt;a href=&quot;http://www.animalhealthinternational.com/&quot;&gt;http://www.animalhealthinternational.com/&lt;/a&gt;</td></tr><tr><td>Acepromazine 10 mg/mL</td><td>Animal Health International</td><td>17640541</td><td></td></tr><tr><td>Xylazine 20 mg/mL</td><td>Animal Health International</td><td>20101547</td><td></td></tr><tr><td>Yohimbine 0.2 mg/mL</td><td>Animal Health International</td><td>14588965</td><td></td></tr><tr><td>Hair Removing Cream</td><td>Veet</td><td></td><td>Sensitive skin solution. Available through local retailers.</td></tr><tr><td>Blunt tip infusion needles</td><td>Sai Infusion Technology</td><td>B14-50</td><td>&lt;a href=&quot;http://www.sai-infusion.com/collections/blunt-needles&quot;&gt;http://www.sai-infusion.com/collections/blunt-needles&lt;/a&gt;</td></tr><tr><td>Veterinary Pulse Oximeter</td><td>EdanUSA</td><td>VE-H100B</td><td>&lt;a href=&quot;http://www.edanusa.com/Product/VE-H100B-Veterinary-Pulse-Oximeter.html&quot;&gt;http://www.edanusa.com/Product/VE-H100B-Veterinary-Pulse-Oximeter.html&lt;/a&gt;</td></tr><tr><td>Warm Water Pump</td><td>Gaymar</td><td>TP700</td><td></td></tr><tr><td>Warm Water Blanket</td><td>Animal Health International</td><td>21232696</td><td>Maxi-Therm Lite Warming Pads</td></tr><tr><td>Ultrasound system</td><td>VisualSonics</td><td>Vevo 2100</td><td></td></tr></tbody></table>

intraductal delivery to the rabbit mammary gland

乳癌のためのローカライズされた管内の治療は、腫瘍への効率的な送達および減少全身毒性及び副作用1、2、3、4、5、6、7を含む潜在的な利点を提供します。これらの処理は、より広く適用することができる前に、しかし、いくつかの課題が残っています。適切な動物モデルにおける管内治療法の開発と検証は、患者のための管内治療戦略の開発を促進します。マウス乳腺広く乳腺発達および腫瘍形成のモデル系として使用されてきたが、解剖学的構造は、人間の腺と区別されます。大きな動物モデルは、ウサギのような乳腺構造と管内治療の開発のためのより良好なモデルとして役立つことができます。 C INマウスにontrast、ここで10乳管の木は空間的に分離した乳首の各終端は、ウサギの乳腺は、より密接1乳頭内の別の開口部を通ってその出口複数の重複乳管システムで、人間の腺に似ている、体軸に沿って分布しています。ここでは、直接ウサギ乳管に、高解像度の超音波画像で配信自体の可視化のための試薬の送達のための低侵襲的な方法を提示します。

ここでは、ウサギの乳管の造影試薬の管内送達は、組織（ 図2）への外傷なしで達成することができることを示しています。ウサギでは、4つの別々の導管システムは、1つの乳頭に収束するので、アクセスし、この方法を使用して個別に撮像することができます。個々の管の開口部は、容易に可視化されます。 図2Bのカニューレを挿入し、ダ?...

Watch this Scientific Journal Video about Intraductal Delivery to the Rabbit Mammary Gland at JoVE.com

Intraductal Delivery to the Rabbit Mammary Gland

Here, we describe a technique for the localized delivery of reagents to the rabbit mammary gland via an intraductal injection. In addition, we describe a protocol for visualization and the confirmation of delivery by high-resolution ultrasound imaging of contrast agents.

ウサギの乳腺への管内配達

Localized intraductal treatments for breast cancer offer potential advantages, including efficient delivery to the tumor and reduced systemic toxicity and ...

intraductal-delivery-to-the-rabbit-mammary-gland

Research

JoVE Journal

Bioengineering

9.3K ビュー.  The University of Texas at Austin. Here, we describe a technique for the localized delivery of reagents to the rabbit mammary gland via an intraductal injection. In addition, we describe a protocol for visualization and the confirmation of delivery by high-resolution ultrasound imaging of contrast agents. 

ビデオ: Intraductal Delivery to the Rabbit Mammary Gland

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model

Assisted reproductive techniques (ARTs), such as in vitro embryo culture or embryo cryopreservation, affect natural development patterns with perinatal and postnatal consequences. To ensure the innocuousness of ART applications, studies in animal models are necessary. In addition, as a last step, embryo development studies require evaluation of their capacity to develop full-term healthy offspring. Here, embryo transfer to the uterus is indispensable to perform any ARTs-related experiment.
The rabbit has been used as a model organism to study mammalian reproduction for over a century. In addition to its phylogenetic proximity to the human species and its small size and low maintenance cost, it has important reproductive characteristics such as induced ovulation, a chronology of early embryonic development similar to humans and a short gestation that allow us to study the consequences of ART application easily. Moreover, ARTs (such as intracytoplasmic sperm injection, embryo culture, or cryopreservation) are applied with suitable efficiency in this species.
Using the laparoscopic embryo transfer technique and the cryopreservation protocol presented in this article, we describe 1) how to transfer embryos through an easy, minimally invasive technique and 2) an effective protocol for long-term storage of rabbit embryos to provide time-flexible logistical capacities and the ability to transport the sample. The outcomes obtained after transferring rabbit embryos at different developmental stages indicate that morula is the ideal stage for rabbit embryo recovery and transfer. Thus, an oviductal embryo transfer is required, justifying the surgical procedure. Furthermore, rabbit morulae are successfully vitrified and laparoscopically transferred, proving the effectiveness of the described techniques.

Assisted reproductive techniques (ARTs) are in continuous evaluation to improve outcomes and reduce the associated risks. This manuscript describes a minimally invasive embryo transfer procedure with an efficient cryopreservation protocol that allows the use of rabbits as an ideal animal model of human reproduction.

ウサギモデルにおける最適胚期における低侵襲胚移植と胚ガラス化

Assisted reproductive techniques (ARTs), such as in vitro embryo culture or embryo cryopreservation, affect natural development patterns with perinatal ...

発生生物学

Intraductal Delivery and X-ray Visualization of Ethanol-Based Ablative Solution for Prevention and Local Treatment of Breast Cancer in Mouse Models

Breast cancer is the most prevalent cancer and the second-leading cause of cancer-related death for women in the USA. For high-risk women, prophylactic mastectomy is the most effective primary prevention strategy. Prophylactic mastectomy is an aggressive surgical procedure that completely removes the mammary epithelial cells from which breast cancer arises along with the surrounding tissue. We seek to develop a minimally invasive intraductal procedure as an alternative to prophylactic mastectomy to locally ablate the mammary epithelial cells before they can become malignant. We and others have developed an intraductal delivery procedure to reach and treat these epithelial cells in rodent models of breast cancer. While the mouse mammary gland with a single non-anastomosed ductal tree opening at the nipple has a much less complex and tortuous architecture than the human breast, chemically induced and genetically engineered mouse models of breast cancer are valuable to produce proof-of-concept studies of new preventative strategies. Here, we describe a procedure for intraductal delivery of an ethanol-based ablative solution containing micro-CT/X-ray tantalum-based contrast agent within the mouse mammary ductal tree for the therapeutic purpose of primary prevention of breast cancer. Intraductal delivery of aqueous reagents (e.g., cytotoxic compounds, siRNAs, AdCre) has been previously described in mouse models. Thus, we focus our protocol description on methodological modifications and unique experimental considerations for optimizing delivery of ethanol, for minimizing local and systemic side effects of ethanol administration, and for in vivo visualization of ductal tree filling via micro-CT/fluoroscopy imaging. Visualization of the ductal tree immediately after injection of a contrast-containing solution allows for confirmation of complete filling or unsuccessful outcomes such as underfilling or overfilling. This procedure can be applied for delivery and imaging of other ablative compounds aimed at either preventing tumor formation or locally treating early-stage tumors accessible via the ductal tree.

A method of intraductal injection of reagents for an ethanol-based ablative solution to the mouse mammary ductal tree for in vivo imaging and breast cancer prevention is described. Injection directly into the nipple opening allows for targeting mammary epithelial cells with minimal collateral tissue damage.

マウスモデルにおける乳癌の予防および局所治療のためのエタノールベースのアブレーション溶液の管内送達およびX線可視化

Breast cancer is the most prevalent cancer and the second-leading cause of cancer-related death for women in the USA. For high-risk women, prophylactic ...

がん研究

X-Ray Visualization of Intraductal Ethanol-Based Ablative Treatment for Prevention of Breast Cancer in Rat Models

There are still a limited number of primary interventions for prevention of breast cancer. For women at a high risk of developing breast cancer, the most effective intervention is prophylactic mastectomy. This is a drastic surgical procedure in which the mammary epithelial cells that can give rise to breast cancer are completely removed along with the surrounding tissue. The goal of this protocol is to demonstrate the feasibility of a minimally invasive intraductal procedure that could become a new primary intervention for breast cancer prevention. This local procedure would preferentially ablate mammary epithelial cells before they can become malignant. Intraductal methods to deliver solutions directly to these epithelial cells in rodent models of breast cancer have been developed at Michigan State University and elsewhere. The rat mammary gland consists of a single ductal tree that has a simpler and more linear architecture compared to the human breast. However, chemically induced rat models of breast cancer offer valuable tools for proof-of-concept studies of new preventive interventions and scalability from mouse models to humans. Here, a procedure for intraductal delivery of an ethanol-based ablative solution containing tantalum oxide nanoparticles as X-ray contrast agent and ethyl cellulose as gelling agent into the rat mammary ductal tree is described. Delivery of aqueous reagents (e.g., cytotoxic compounds, siRNAs, AdCre) by intraductal injection has been described previously in mouse and rat models. This protocol description emphasizes methodological changes and steps that pertain uniquely to delivering an ablative solution, formulation consideration to minimize local and systemic side effects of the ablative solution, and X-ray imaging for in vivo assessment of ductal tree filling. Fluoroscopy and micro-CT techniques enable to determine the success of ablative solution delivery and the extent of ductal tree filling thanks to compatibility with the tantalum-containing contrast agent.

A procedure for the delivery of a chemical ablative solution to the rat mammary ductal tree for image-guided preventive treatment of breast cancer is described. Mammary epithelial cells can be targeted with minimal collateral tissue damage through cannulation directly into the nipple opening and intraductal infusion of a 70% ethanol-based ablative solution.

ラットモデルにおける乳癌予防のための乳管内エタノール焼灼治療のX線可視化

There are still a limited number of primary interventions for prevention of breast cancer. For women at a high risk of developing breast cancer, the most ...

In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection

Mouse mammary glands comprise ductal trees, which are lined by epithelial cells and have one opening at the tip of each nipple. The epithelial cells play a major role in mammary gland function and are the origin of most mammary tumors. Introducing genes of interest into mouse mammary epithelial cells is a critical step in evaluating gene function in epithelial cells and generating mouse mammary tumor models. This goal can be accomplished through the intraductal injection of a viral vector carrying the genes of interest into the mouse mammary ductal tree. The injected virus subsequently infects mammary epithelial cells, bringing in the genes of interest. The viral vector can be lentiviral, retroviral, adenoviral, or adenovirus-associated viral (AAV). This study demonstrates how a gene of interest is delivered into mammary epithelial cells through mouse mammary intraductal injection of a viral vector. A lentivirus carrying GFP is used to show stable expression of a delivered gene, and a retrovirus carrying Erbb2 (HER2/Neu) is used to demonstrate oncogene-induced atypical hyperplastic lesions and mammary tumors.

In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection

The present protocol describes intraductal injection of viral vectors via the teat to deliver genes of interest into the mammary epithelial cells.

インビボ 乳腺内注射によるマウス乳腺上皮細胞への遺伝子導入

Mouse mammary glands comprise ductal trees, which are lined by epithelial cells and have one opening at the tip of each nipple. The epithelial cells play ...

Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits (Oryctolagus cuniculus)

Due to similarities in placentation and antibody transfer with humans, rabbits are an excellent model of maternal immunization. Additional advantages of this research model are the ease of breeding and sample collection, relatively short gestation period, and large litter sizes. Commonly assessed routes of immunization include subcutaneous, intramuscular, intranasal, and intradermal. Nonterminal sample collection for the chronological detection of the immunologic responses to these immunizations include the collection of blood, from both dams and kits, and milk from the lactating does. In this article, we will demonstrate techniques our lab has utilized in studies of maternal immunization in New Zealand White rabbits (Oryctolagus cuniculus), including intranasal immunization and milk collection.

Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits Oryctolagus cuniculus

This article describes and demonstrates the administration of intranasal vaccines and the collection of milk from lactating rabbits (Oryctolagus cuniculus) as a means to assess mucosal immunity in a translationally appropriate model of maternal immunization.

ニュージーランド白ウサギにおける母体免疫の研究における鼻腔内免疫とミルクコレクション (オリクトラグス・キュニクルス)

Due to similarities in placentation and antibody transfer with humans, rabbits are an excellent model of maternal immunization. Additional advantages of ...

免疫・感染学

Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland

Herein we describe a protocol to deliver various reagents to the mouse mammary gland via intraductal injections. Localized drug delivery and knock-down of genes within the mammary epithelium has been difficult to achieve due to the lack of appropriate targeting molecules that are independent of developmental stages such as pregnancy and lactation. Herein, we describe a technique for localized delivery of reagents to the mammary gland at any stage in adulthood via intraductal injection into the nipples of mice. The injections can be performed on live mice, under anesthesia, and allow for a non-invasive and localized drug delivery to the mammary gland. Furthermore, the injections can be repeated over several months without damaging the nipple. Vital dyes such as Evans Blue are very helpful to learn the technique. Upon intraductal injection of the blue dye, the entire ductal tree becomes visible to the eye. Furthermore, fluorescently labeled reagents also allow for visualization and distribution within the mammary gland. This technique is adaptable for a variety of compounds including siRNA, chemotherapeutic agents, and small molecules.

A protocol for the non-invasive intraductal delivery of aqueous reagents to the mouse mammary gland is described. The method takes advantage of localized injection into the nipples of mammary glands targeting mammary ducts specifically. This technique is adaptable for a variety of compounds including siRNA, chemotherapeutic agents and small molecules.

マウス乳腺に局在薬物送達のための管内注入

Herein we describe a protocol to deliver various reagents to the mouse mammary gland via intraductal injections. Localized drug delivery and knock-down of ...

生物学

Simplifying Transcutaneous Intratracheal Drug Delivery in the Newborn Preterm Rabbit

Intratracheal (IT) drug delivery allows the direct delivery of pharmaceutical substances to the lung, maximizing potential pulmonary benefit and minimizing systemic drug exposure. The transcutaneous technique is simple and allows for the IT delivery of substances to the lung of prematurely born rabbits shortly after birth. Newborn pups are anesthetized with inhaled Isoflurane before being placed in a supine position with the neck extended. The larynx is identified and stabilized before transcutaneous placement of a 26-gauge (G) catheter into the trachea. Following catheterization of the trachea, a 30 G blunt needle attached to a Hamilton syringe is introduced into the IT catheter and is used for delivering a precise volume into the trachea during spontaneous respiration. After the IT injection is completed, the needle and catheter are withdrawn, and the pup is allowed to recover from anesthesia. Transcutaneous IT injection delivers a large proportion of the injected substance to the lung, with the majority remaining in the lung 3 hours after the intervention. The injections are well tolerated from the day of birth and can be repeated for multiple consecutive days without influencing survival. This technique can be used to investigate the effect of pharmaceutical agents on lung development and in the prevention of neonatal lung injury in preterm rabbits.

Transcutaneous intratracheal injection allows for effective intrapulmonary drug delivery during spontaneous respiration. Single and multiple injections are well tolerated with no effect on survival. The technique is simple to perform and can examine the effect of substances on lung development and the prevention of lung injury in newborn rabbits.

Intratracheal (IT) drug delivery allows the direct delivery of pharmaceutical substances to the lung, maximizing potential pulmonary benefit and ...

医学

Pregnancy and Nursing Management for Embryo-Transferred and Genetically Modified Rabbits

With the advancement of scientific research, the demand for gene-edited rabbit models is increasing. However, there are limited pregnancy and feeding management systems for gene-edited rabbits, leading to low survival rates among gene-edited rabbits prepared by many inexperienced researchers. Therefore, proper guidance is essential. This article summarizes the pregnancy and feeding practices for genetically modified rabbits developed in the author's laboratory and outlines a set of fundamental processes. These include pregnancy diagnosis, antenatal care, midwifery, assisted breastfeeding, weaning, and other procedures, along with the rescue and care of weak newborn rabbits. Compared to the traditional natural childbirth and nurturing methods used in rabbit farms, this approach involves more refined management, requiring additional time and effort but significantly increasing the survival rate of suckling rabbits. The methods described in this article are suitable for most laboratory breeding scenarios involving gene-edited or embryo-transferred rabbits and provide a straightforward and effective reference for other researchers.

This protocol describes a pregnancy and feeding management technique for embryo-transferred and genetically modified rabbits, aimed at reducing newborn rabbit mortality and enhancing the preparation efficiency of gene-edited rabbits.

胚移植ウサギおよび遺伝子組換えウサギの妊娠・看護管理

With the advancement of scientific research, the demand for gene-edited rabbit models is increasing. However, there are limited pregnancy and feeding ...

Intraductal Injection: Delivering Injection Mix into the Ducts of the Mouse Mammary Gland

Source: Xiang, D., et al. <a href="https://www.jove.com/v/59502/modeling-breast-cancer-via-an-intraductal-injection-cre-expressing">Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland.</a> J. Vis. Exp (2019).
This video describes an intraductal injection technique where we deliver injection mix into the ducts of mouse mammary glands.

管内注射:マウス乳腺の管に注射ミックスを送達する

Source: Xiang, D., et al. Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland. J. Vis. Exp ...

JoVE Encyclopedia of Experiments

乳がん

手順と技術

Rabbit Intraductal Injection: Localized Delivery of Solution of Interest into the Rabbit Mammary Gland

Source: Clark et. al., <a href="https://www.jove.com/v/55209/intraductal-delivery-to-the-rabbit-mammary-gland">Intraductal Delivery to the Rabbit Mammary Gland.</a> J. Vis. Exp. (2017).
In this video we describe a technique of administering a solution of interest into the mammary duct of a rabbit and confirm the delivery by high-resolution ultrasound imaging of contrast agents.

ウサギの乳管内注射:ウサギの乳腺への目的の溶液の局所的な送達

Source: Clark et. al., Intraductal Delivery to the Rabbit Mammary Gland. J. Vis. Exp. (2017).
In this video we describe a technique of administering a ...

ウサギの乳腺への管内配達

要約

さらに動画を探す

この動画の章

ウサギモデルにおける最適胚期における低侵襲胚移植と胚ガラス化

マウスモデルにおける乳癌の予防および局所治療のためのエタノールベースのアブレーション溶液の管内送達およびX線可視化

ラットモデルにおける乳癌予防のための乳管内エタノール焼灼治療のX線可視化

インビボ乳腺内注射によるマウス乳腺上皮細胞への遺伝子導入

ニュージーランド白ウサギにおける母体免疫の研究における鼻腔内免疫とミルクコレクション (オリクトラグス・キュニクルス)

マウス乳腺に局在薬物送達のための管内注入

Simplifying Transcutaneous Intratracheal Drug Delivery in the Newborn Preterm Rabbit

胚移植ウサギおよび遺伝子組換えウサギの妊娠・看護管理

管内注射:マウス乳腺の管に注射ミックスを送達する

ウサギの乳管内注射:ウサギの乳腺への目的の溶液の局所的な送達

ウサギの乳腺への管内配達

要約

さらに動画を探す

この動画の章

ウサギモデルにおける最適胚期における低侵襲胚移植と胚ガラス化

マウスモデルにおける乳癌の予防および局所治療のためのエタノールベースのアブレーション溶液の管内送達およびX線可視化

ラットモデルにおける乳癌予防のための乳管内エタノール焼灼治療のX線可視化

インビボ 乳腺内注射によるマウス乳腺上皮細胞への遺伝子導入

ニュージーランド白ウサギにおける母体免疫の研究における鼻腔内免疫とミルクコレクション (オリクトラグス・キュニクルス)

マウス乳腺に局在薬物送達のための管内注入

Simplifying Transcutaneous Intratracheal Drug Delivery in the Newborn Preterm Rabbit

胚移植ウサギおよび遺伝子組換えウサギの妊娠・看護管理

管内注射:マウス乳腺の管に注射ミックスを送達する

ウサギの乳管内注射:ウサギの乳腺への目的の溶液の局所的な送達

インビボ乳腺内注射によるマウス乳腺上皮細胞への遺伝子導入