タンパク質-タンパク質相互作用や DNA 損傷速度論を研究する共焦点の顕微鏡検査の技術を高度な

The overall goal of these experiments is to induce DNA lesions using local micro irradiation of cells and to observe the recruitment of proteins involved in DNA repair processes.This method can help us to understand a very complex and profound question such as, how normal and cancer cells respond to DNA injury.The main advantage of this technique is that we are able to study the DNA damage response in individual live cells.Demonstrating the procedure will be by Sona Legartova, a post-doc and Jana Suchankova, a PhD student from my laboratory.To begin, cultivate HeLa cells in DMAM supplemented with 10%FBS and appropriate antibiotics.Incubate the cells at 37