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HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

8.1K Views

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10:10 min

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March 31st, 2019

DOI :

10.3791/59382-v

March 31st, 2019

•

8,094 Views

Huacheng Luo¹, Amin Sobh², Christopher D. Vulpe², Edmond Brewer¹, Sinisa Dovat¹, Yi Qiu³, Suming Huang¹

¹Department of Pediatrics, Pennsylvania State University College of Medicine, ²Department of Physiological Sciences, University of Florida, ³Department of Anatomy and Cell Biology, University of Florida

章

文字起こし

要約

さらに動画を探す

CRISPR sgRNA Library

CTCF Boundaries

HOX Gene Regulation

Pooled SgRNA Screening

Lentiviral Transduction

MOLM13 Cells

Puromycin Selection

MOI Optimization

Noncoding Regulatory Elements

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