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13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement (Video) | JoVE

DOI :

10.3791/200017-v

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4:55 min

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May 19th, 2023

May 19th, 2023

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179 Views

Tenzin Tseyang^1*, Jonathan Valeros^1*, Paula Vo^1*, Jessica B. Spinelli^1,2

¹Program in Molecular Medicine, University of Massachusetts Chan Medical School, ²Cancer Center, University of Massachusetts Chan Medical School

* These authors contributed equally

Tags

Keywords 13C4 aspartate

Stable Isotope Tracing

SDH Activity Measurement

143B Human Osteosarcoma Cells

Metabolite Extraction

Vacuum Concentration

Liquid Chromatography

Mass Spectrometry

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