evaluation of infection in major organs in the periprosthetic joint infection (pji) mouse model

칸디다 알비칸스(Candida albicans) 생물막 관련 PJI에 대한 마우스 모델 확립

Candida albicans (C. albicans) commensally reside in many parts of the human body1, which is also the most common opportunistic pathogen that causes life-threatening invasive fungal infections, especially in immunocompromised patients2,3. C. albicans can transform between yeast and mycelium states as a polymorphic fungus. The mycelium state exhibits higher virulence, stronger adhesion, and invasion of cells and tissues4,5. Besides, C. albicans can form biofilms on the surfaces of biomedical materials such as dentures, catheters, and stents1,6,7. The dense three-dimensional structure of biofilms restricts the infiltration of antifungal drugs, expresses drug-resistant genes, and down-regulates the metabolism of fungal cells to resist immune system clearance6,7. Therefore, biofilms-related infections&#160;are quite challenging in clinics8.
Staphylococcus aureus, coagulase-negative staphylococcus, and enterobacter are the main pathogens causing PJI9. Although the incidence of fungal PJI is relatively low (about 1%)10, the treatment cost of fungal PJI is higher11, the treatment cycle is longer11, and the treatment success rate is lower10 than bacterial PJI. In recent years, the incidence of fungal PJI has been increasing year-by-year10. Candida PJI accounts for 77%-84% of fungal PJI10,12, and C. albicans is the most common in Candida (54%). Therefore, fungal PJI needs to be studied.
Currently, PJI is treated via revision surgery by (1) removing the infected implant, (2) debridement, (3) antimicrobial treatment, and (4) reimplantation. After thorough debridement, an antibiotic containing bone cement is placed, and the patient is treated with antibiotics systemically for more than 6 weeks to effectively control the infection before a new implant is placed13. However, this method cannot fully eliminate pathogens in tissues, and recurrent infections treated with long-term antimicrobial therapy are highly likely to develop in drug-resistant strains14,15,16.
Establishing animal models of PJI is important for the research and development of new drugs or therapeutics for PJI. In the development of PJI, large dead spaces are formed around the prosthesis, leading to the formation of hematomas, which further block the blood supply of the surrounding tissues and impair the effect of antibiotics11,15. Due to the difficulty in mimicking the surrounding environment of the prosthesis, traditional animal models cannot accurately simulate the actual situation of PJI17,18.
In this paper, a C. albicans biofilm-associated PJI model in mice was constructed by using a clinically widely used titanium-nickel wire to simulate joint implants19,20. This PJI model exhibits the advantages of easy operation, high successful rate, high repeatability, and high clinical correlation. It is expected to be an important model for studying the prevention and treatment of C. albicans biofilm-related PJI.

저자 스포트라이트: Candida albicans Biofilm-Associated Prosthetic Joint Infections에 대한 연구 발전 

마우스의 보철 관절 Candida albicans 감염 모델

In this study of Candida albicans biofilm-associated peri-prosthetic joint, Candida albicans infection model in mice was constructed, using titanium-nickel wire, commonly used to simulate joint implants. This PJI model is easy to operate, has a high success rate, and is clinically relevant. It will help understanding the prevention and treatment of Candida albicans biofilm-related PJI.Traditional animal models cannot accurately simulate the actual situation of PJI, due to the complex environment surrounding the processes. Candida albicans biofilm-related mouse PJI model, can address the research gap. The establishment of PJI animal model is very important for the research and the development of new drugs or therapeutics for PJI.The Candida albicans biofilm-associated mouse PJI model established in this paper is expected to be an important model for the prevention and treatment of Candida albicans biofilm-associated PJI.

Watch this Scientific Journal Video about Evaluation of Infection in Major Organs in the Periprosthetic Joint Infection PJI Mouse Model at JoVE.com

Evaluation of Infection in Major Organs in the Periprosthetic Joint Infection (PJI) Mouse Model  

PJI(Periprosthetic Joint Infection) 마우스 모델에서 주요 장기의 감염 평가

시작하려면 칸디다 알비칸스(candida albicans) 생물막 관련 PJI 마우스 모델을 만듭니다. 모델 설립 후 14일이 지나면 쥐를 안락사시키고 신장, 간, 비장을 적출합니다. 각 장기에 대해 500마이크로리터의 멸균 생리식염수를 투입합니다.섭씨 4도의 균질기에서 조직을 갈아줍니다. 그런 다음 준비된 균질액 100마이크로리터를 YPD 플레이트에 넣고 구부러진 막대를 사용하여 고르게 펼칩니다. YPD 플레이트를 섭씨 37도 인큐베이터에 거꾸로 놓고 48시간 동안 놓습니다.부화가 끝나면 판을 육안으로 검사하여 눈에 보이는 식민지를 세십시오.

evaluation-infection-major-organs-periprosthetic-joint-infection-pji

Research

JoVE Journal

Immunology and Infection

Evaluation of Infection in Major Organs in the Periprosthetic Joint Infection (PJI) Mouse Model

61 조회수.  Xi’an Jiaotong University. 시작하려면 칸디다 알비칸스(candida albicans) 생물막 관련 PJI 마우스 모델을 만듭니다. 모델 설립 후 14일이 지나면 쥐를 안락사시키고 신장, 간, 비장을 적출합니다. 각 장기에 대해 500마이크로리터의 멸균 생리식염수를 투입합니다.섭씨 4도의 균질기에서 조직을 갈아줍니다. 그런 다음 준비된 균질액 100마이크로리터를 YPD 플레이트에 넣고 구부러진 막대를 사용하여 고르?...

비디오: Evaluation of Infection in Major Organs in the Periprosthetic Joint Infection (PJI) Mouse Model  

A Periprosthetic Joint Candida albicans Infection Model in Mouse

Periprosthetic joint infection (PJI) is one of the common infections caused by Candida albicans (C. albicans), which increasingly concerns surgeons and scientists. Generally, biofilms that can shield C. albicans from antibiotics and immune clearance are formed at the infection site. Surgery involving the removal of the infected implant, debridement, antimicrobial treatment, and reimplantation is the gold standard for the treatment of PJI. Thus, establishing animal PJI models is of great significance for the research and development of new drugs or therapeutics for PJI. In this study, a smooth nickel-titanium alloy wire, a widely used implant in orthopedic clinics, was inserted into the femoral joint of a C57BL/6 mouse before the C. albicans were inoculated into the articular cavity along the wire. After 14 days, mature and thick biofilms were observed on the surface of implants under a scanning electronic microscope (SEM). A significantly reduced bone trabecula was found in the H&amp;E staining of the infected joint specimens. To sum up, a mouse PJI model with the advantages of easy operation, high successful rate, high repeatability, and high clinical correlation was established. This is expected to be an important model for clinical studies of C. albicans biofilm-related PJI prevention.

Periprosthetic joint infection (PJI) caused by dangerous pathogens is common in clinical orthopedics. Existing animal models cannot accurately simulate the actual situation of PJI. Here, we established a Candida albicans biofilm-associated PJI mouse model to research and develop new therapeutics for PJI.

Periprosthetic joint infection (PJI) is one of the common infections caused by Candida albicans (C. albicans), which increasingly concerns surgeons and ...