Establishing an Alzheimer's Disease Rat Model Through an Amyloid-Beta Peptide Injection

Begin with an immobilized, anesthetized rat with its skull exposed.

Locate the bregma, mark three reference points, and carefully drill the holes.  Clean the area.

Through one of the holes, inject a growth factor into the anterodorsal thalamic nucleus near the hippocampus.

Secure two screws in two of the holes, insert a guide cannula into the third hole, and fix them with dental cement. Cover the guide cannula with a dummy cannula.

The next day, insert the internal cannula into the guide cannula and connect it to the injection system.

Regularly inject amyloid-beta peptides and aluminum trichloride into the brain's lateral ventricle to facilitate their diffusion in the hippocampus.

Later, remove the cannula implantation system. Disinfect the area, fill the holes with bone cement, and suture the incision.

Over time, the amyloid-beta peptides aggregate into toxic plaques.

The injected growth factor and aluminum trichloride also enhance plaque formation around the hippocampal neurons, which are crucial for memory.

This leads to neuronal death, causing memory impairment similar to Alzheimer's disease.