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Antimicrobial Activity and Toxicity Assessment of Compounds Targeting Mycobacterium abscessus in Macrophage Infection Assays
Gabriel S. Oliveira1,2, Clara M. Bento1,3,4, António Pombinho1,3, Rita Reis1,3, Kevin Van Calster5,6, Linda De Vooght5, André F. Maia1,3, Paul Cos5, Maria Salomé Gomes1,2, Tânia Silva1,2
1i3S – Instituto de Investigação e Inovação em Saúde da Universidade do Porto, 2ICBAS – Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 3IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, 4Programa Doutoral em Biologia Molecular e Celular (MCBiology), Instituto de Ciências Biomédicas Abel Salazar da Universidade do Porto, 5Laboratory for Microbiology, Parasitology and Hygiene (LMPH), 6Department of Infectious Diseases in Humans, Immune Response, Sciensano
Retroviral CRISPR/Cas9-Mediated Gene Targeting for the Study of Th17 Differentiation in Vitro
1Shanghai Immune Therapy Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 2Zhejiang University-University of Edinburgh Institute (ZJU-UoE Institute), Zhejiang University School of Medicine, Zhejiang University, 3Institute of Immunology and Department of Rheumatology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine
Assay Development for High-Throughput Drug Screening Against Mycobacteria
Gabriel S. Oliveira1,2, Clara M. Bento1,3,4, António Pombinho1,3, Rita Reis1,3, Kevin Van Calster5,6, Linda De Vooght5, André F. Maia1,3, Paul Cos5, Maria Salomé Gomes1,2, Tânia Silva1,2
1i3S – Instituto de Investigação e Inovação em Saúde da Universidade do Porto, 2ICBAS – Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 3IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, 4Programa Doutoral em Biologia Molecular e Celular (MCBiology), Instituto de Ciências Biomédicas Abel Salazar da Universidade do Porto, 5Laboratory for Microbiology, Parasitology and Hygiene (LMPH), 6Department of Infectious Diseases in Humans, Immune Response, Sciensano
Stimulation and Incubation of Whole Blood Samples for Antigen-Specific Analysis of Host Immunity to Fungal and Viral Pathogens
Lukas Page1, Chris D. Lauruschkat2, Kevin Dennehy1, Eva Loell1, Beeke Tappe2, Andre Fuchs3, Sebastian Wurster*4, Reinhard Hoffmann*1
1Institute for Laboratory Medicine and Microbiology, University Hospital Augsburg, 2Department of Internal Medicine II, University Hospital of Wuerzburg, 3Internal Medicine III - Gastroenterology and Infectious Diseases, University Hospital Augsburg, 4Department of Infectious Diseases, Infection Control and Employee Health, MD Anderson Cancer Center, University of Texas Houston
Preparation of Whole Blood Samples for Flow Cytometry and Cytokine Analysis to Determine Antigen-Specific Host Immunity to Fungal and Viral Pathogens
Lukas Page1, Chris D. Lauruschkat2, Kevin Dennehy1, Eva Loell1, Beeke Tappe2, Andre Fuchs3, Sebastian Wurster*4, Reinhard Hoffmann*1
1Institute for Laboratory Medicine and Microbiology, University Hospital Augsburg, 2Department of Internal Medicine II, University Hospital of Wuerzburg, 3Internal Medicine III - Gastroenterology and Infectious Diseases, University Hospital Augsburg, 4Department of Infectious Diseases, Infection Control and Employee Health, MD Anderson Cancer Center, University of Texas Houston
Development of a Polymicrobial Colony Biofilm Model to Test Antimicrobials in Cystic Fibrosis
1School of Life Sciences, Biodiscovery Institute, University of Nottingham, 2MiDx Ltd, Biodiscovery Institute, University of Nottingham, 3School of Life Sciences, Queen's Medical Centre, University of Nottingham, 4National Biofilms Innovation Centre, School of Life Sciences, Biodiscovery Institute, University of Nottingham
Loading of Primary Mouse CD4+ T Cells and Coating of Protein G Magnetic Beads with Antibodies for Local Ca2+ Imaging
Fynn Gerlach*1, Franziska Möckl*1, Dejan Kovacevic1,2,3, Valerie J. Brock1, Riekje Winzer4, Lina Meyer2,3, David Lohr2,3, Lena-Marie Woelk2,3, Eva Tolosa4, René Werner2,3, Björn-Philipp Diercks1
1The Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Centre Hamburg-Eppendorf, 2Department of Applied Medical Informatics, University Medical Centre Hamburg-Eppendorf, 3Department of Computational Neuroscience, University Medical Centre Hamburg-Eppendorf, 4Department of Immunology, University Medical Centre Hamburg-Eppendorf
Detecting Ca2+ Microdomains on a Subcellular Level in Primary Murine T Cells Using Fluorescence Microscopy
Fynn Gerlach*1, Franziska Möckl*1, Dejan Kovacevic1,2,3, Valerie J. Brock1, Riekje Winzer4, Lina Meyer2,3, David Lohr2,3, Lena-Marie Woelk2,3, Eva Tolosa4, René Werner2,3, Björn-Philipp Diercks1
1The Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Centre Hamburg-Eppendorf, 2Department of Applied Medical Informatics, University Medical Centre Hamburg-Eppendorf, 3Department of Computational Neuroscience, University Medical Centre Hamburg-Eppendorf, 4Department of Immunology, University Medical Centre Hamburg-Eppendorf
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