<a href="https://www.jove.com/video/50175">최소 홍반 량 (MED) 시험</a> 캐롤린 J. Heckman 1, 레이첼 챈들러, 재클린 D. KLOSS 3, 에이미 벤슨 2, 데보라 루니 2 Teja Munshi 1, 수잔 D. Darlow 1, 클리포드 펄리스 4, 샤론 L. Manne 5, 데이비드 W. Oslin 2 1 암 예방 및 제어 프로그램, 폭스 체이스 암 센터, 정신과 교실 2, 펜실베니아 대학, 심리학의 3 부, 드렉 셀 대학교 의과 4 부, 폭스 체이스 암 구보, 5 암 예방 및 제어 프로그램의 암 연구소 뉴저지 이 문서 determi하기 위해 최소 홍반 량 (MED) 테스트를 수행하는 방법에 대해 설명합니다네브래스카 개인에게 투여시 홍반 (굽기) 원인이됩니다 자외선 방사선의 낮은 복용량. <a href="https://www.jove.com/video/50344">측정 세포 내 칼슘 2 개의 기술하여 인간 정자의 + 변경 : 기존 Fluorometry은 흐름 Fluorometry, 유동 세포 계측법 및 단일 세포 이미징을 중지</a> 에스페란자 마타 - 마르티네즈 1, 오마르 호세 1 폴리나 토레스 - 로드리게스 1, 알레 SOLIS-로페스 1, 아나 A. 산체스 - Tusie 1 Yoloxochitl 산체스 - 게바라 1, 마르셀 B. 트레비노 2, 클라우디아 L. 트레비노 1 1 Departamento 드 Genética 델 Desarrollo Y Fisiología 분자, INSTITUTO 드 Biotecnología - 국립 대학교 AUTONOMA 드 멕시코, 2 수학 및 과학 교실 <stroNG&gt; 에디슨 주립 대학 내 Ca 2 + 역학 정자 생리학 및 칼슘 2 +에 민감한 형광 염료를 연구하는 다양한 도구를 구성에 매우 중요합니다. 인구 실험 (fluorometry 및 흐름 fluorometry 중지) 및 단일 세포 실험 (유동 세포 계측법 및 단일 세포 이미징)는 인간의 정자 세포의 시공간적 [칼슘 2 +] 변경 내용을 추적하는 데 사용됩니다. <a href="https://www.jove.com/video/50492">전기 방사 재조합 거미 실크 단백질의 부직포 메쉬를 포함하여 공기 정화 장치</a> 그레고르 랭, 스테판 Jokisch, 토마스 Scheibel 생체 재료 연구 그룹, 바이로이트 대학 거미 실크 섬유는 특별한 기계적 특성을 표시합니다. 엔지니어링 Araneus diadematus 피브로인 4 (eADF4)는 전기 방사를 사용하여 부직포 메쉬로 처리 할 수 있습니다. 그녀의<html> 전자는 eADF4 부직포 메쉬는 공기 여과 장치의 성능을 향상하는 데 사용됩니다. <a href="https://www.jove.com/video/4439">생체 마우스 Neuroepithelium에서 단일 세포 분열의 라이브 영상</a> 카롤리나 Piotrowska-Nitsche 1,2, 타마라 CASPARY 1 1 <a href="/institutions/NA-north-america/US-united-states/GA-georgia/24832-emory-university">인간 유전학 교실에 모리 대학 학부</a> 과학, 실험 발생학 교실 2, IGAB 폴란드어 아카데미 여기에서 우리는 마우스 E8.5 neuroepithelium에서 단일 세포 분열을 추적하는 생체 라이브 영상에 필요한 도구를 개발 <a href="https://www.jove.com/video/50047">고급 3D 형광 현미경을 사용하여 자식 작용의 정량 분석</a> 십t &quot;&gt; 천 A. Changou 1, 디아나 L. 울프슨 2 Balpreet 싱 Ahluwalia는 3, 리처드 J. 굵게 4 HSING-Jien 쿵 5, 프랭크 YS 추앙 6 생화학 및 분자 의학 교실, 바이오 포토닉스 과학 기술, 바이오 포토닉스 과학 기술, 캘리포니아 대학, 데이비스, 바이오 포토닉스 과학 기술 3 NSF 센터, 트롬 쇠 대학 외과학 4 부, 대학 2 NSF 센터 NSF 센터 캘리포니아 데이비스, 생물 화학, 캘리포니아 대학, 데이비스, 6 생화학 교실 및 분자 의학, 캘리포니아 대학, 데이비스의 5학과 자식 작용은 유비쿼터스 세포가 단백질을 분해하고 재활용 할 수 있도록 프로세스와 ORGA입니다nelles. 우리는 시각화하고 작은 정량화 고급 형광 현미경을 적용하지만, autolysosomes로 형성과 autophagosomes와 리소좀의 배포, 자신의 융합 등 자식 작용의 유도와 관련된 필수, 물리적 변화.

관심 없음 충돌 선언하지 않습니다.

may 2013: this month in jove

여기에오고 무엇에 모습입니다 <a href="http://www.jove.com/archive/75">2013년 5월 문제</a> 구상의 실험 연구 - 주피터의가. 처음 왔을 무엇 : 닭 또는 계란? 정액이 근본적인 질문은 재생산의 중요한 구성 요소 부족합니다. 어떤 일이 일어날 수 있기 전에, 정자 세포는 난자에 수영하고 그것으로 융합해야합니다. 이러한 이벤트는 칼슘 이온 (칼슘 2 +)에 의해 매개 신호 전달 경로에 크게 의존하고 있습니다. 따라서 정자의 기능을 연구하기 위해서는 칼슘의 변화 정자의 + 농도를 측정하는 데 유용합니다. 이 칼슘에 민감한 형광 염료에 의해 ​​촉진 될 수 있습니다 <a href="http://www.jove.com/video/50344">. 마타 마르티네스 등</a> 기존 fluorometry, 흐름 fluorometry, 유동 세포 계측법, 그리고 단일 세포 이미징을 중지 : 인간 정자의 역학 + 칼슘 모니터 2 개의 형광 기법 사용합니다. ALO 이동남성의 생식 기관의 다른 부분에 NG, 우리는 전립선 암은 미국 남성에서 암 사망의 주요 원인 중 하나에 관한 기사를 갖추고 있습니다. 인간의 전립선 암 세포에서 효소는 아르기닌 deiminase (ADI)가 최근 자식 작용 세포는 기본적으로 자신의 구성 요소를 먹고있는 세포 죽음의 메커니즘을 유도하는 증명되었다했다. 또한이 메커니즘을 연구하기 위해, <a href="http://www.jove.com/video/50047">Changou 등은.</a> 세포 자식 작용 받아야로 정확하게 형태 학적 변화를 추적 할 수 있도록 정량적 3D 형광 현미경을 사용하여 영상 기반의 접근 방식을 개발했다. 이 기술은 연구원이 전립선 암에 대한 잠재적 인 치료를 연구하는 데 도움이됩니다. 에서 <a href="http://www.jove.com/bioengineering">주피터 생물</a> 과거 기사 박테리아 재조합 거미 실크 단백질을 생산하는 거미의 실크 생산 땀샘을 분리하고 정제 prote를 회전하는 방법을 제안 하였다잠재적 인 응용 생물 의학 (위해 섬유에 기능 <a href="http://www.jove.com/video/2382">. 제프리 외, 2011</a> ; <a href="http://www.jove.com/video/4191">. HSIA 외, 2012</a> ). 이번 달 <a href="http://www.jove.com/video/50492">. 랭 등은</a> 전기 방사 재조합 거미 실크 단백질의 부직포 메쉬 공기 필터 장치를 만들기 : 거미 실크에 대한 새로운 응용 프로그램을 제시한다. 상용 필터 거미 실크의 층으로 코팅하는 경우, 자신의 필터링 효율을 향상시킬 수 있습니다. 저자는 섬유를 electrospin 부직포 실크 메쉬를 치료하고, 스캔 선거 현미경 (SEM)을 사용하여 메쉬를 분석하는 방법을 보여줍니다. 마지막으로, 저자는 공기 침투성 및 필터 효율을 보여줍니다. 에서 <a href="http://www.jove.com/ctm">주피터 임상 및 번역 의학</a> , <a href="http://www.jove.com/video/50175">Heckman 등은.</a> ultravi의 가장 낮은 복용량을 결정하는 방법을 보여줍니다개인에 홍반 (햇볕)가 발생합니다 olet 빛. UV 빛이 종종 건선, 여드름, 습진 등 다양한 피부 질환을 치료하는 데 사용됩니다 모든 사람들이 UV 빛에 똑같이 민감하기 때문에,이 메소드는 관리 할 수​​있는 적절한 복용량을 결정하는 데 도움이 될 수 있습니다. Daavlin 패치는 피부에 UV 노출의 면적과 시간을 모두 제어하는​​ 데 사용됩니다. 다음 날, 피부 색깔의 변화는 홍반을 일으킬 데 필요한 가장 낮은 UV 량을 결정하기 위해 평가됩니다. 에서 <a href="http://www.jove.com/neuro">주피터 신경 과학</a> , <a href="http://www.jove.com/video/4439">Piotrowska-Nitsche 및 CASPARY는</a> 어떻게 배아 마우스 neuroepithelium의 문화 조각을 설명하고 다양한 형광 마커의 라이브 영상을 수행합니다. 이 방법은 연구자가 현장에서 실시간으로 같은 단일 세포 분열 등 세포의 동작을 모니터링 할 수 있습니다. 당신은 5 월 한달 동안 주피터의 하이라이트 중 일부의 미리보기를 했어. 주피터에서, 전체 길이 문서를 참조하는 플러스 더욱 많은 웹 사이트를 방문하십시오 : 구상 실험의 저널.

Watch this Scientific Journal Video about May 2013: This Month in JoVE at JoVE.com

May 2013: This Month in JoVE

여기 구상 실험 (주피터)의 저널의 2013년 5월 문제에서 몇 가지 하이라이트입니다.

2013년 5월 : 주피터에있는이 달

Here's a look at what's coming up in the May 2013 issue of JoVE - The Journal of Visualized Experiments.
What came first: the chicken or the egg? This ...

Without Section

8.2K 조회수.  Massachusetts Eye and Ear. 여기 구상 실험 (주피터)의 저널의 2013년 5월 문제에서 몇 가지 하이라이트입니다.

비디오: May 2013: This Month in JoVE

December 2011: This Month in JoVE

The Journal of Visualized Experiments (JoVE) closes 2011 with a December issue that begins by honoring World's AIDS Day with an article that deals with visualizing the structure of HIV envelope glycoproteins, which are a critical component of the viral infection pathway.
One key method for determining virion envelope structure is cryo-electron tomography. Prior to using this technique, soluble virus must be vitrified on a gold grid - a process that involves adding gold to a viral suspension, transferring that suspension to the grid, and plunging the grid into liquid ethane. The virus particles, present in a thin film of liquid atop the grid, are frozen at a rate greater than 100,000 K per second. Following vitrification, collected samples are loaded into the electron microscope and precisely defined regions on the grid are hit with a focused electron beam, while the grid is tilted. The tilt series that results from these imaging sessions is comprised of hundreds of individual images, which must be aligned and averaged to resolve individual envelope proteins. This computationally intense process often requires computer clusters, capable of remote-access parallel processing, like NIH's Biowulf.
Once completed, 3D models can not only provide insight into the structure of envelope glycoproteins themselves, but can also reveal the manner in which they interact other proteins, like the neutralizing antibody B12, which is information that can greatly influence vaccine design.
This article, from the laboratory of Dr. Sriram Subramaniam involves contributions from twenty authors, including not only graduates students and postdocs, but also middle and highschool students, showing that you are never too young to make an impact in the fight against a disease that has infected over 60 million people, worldwide.
In Clinical and Translational Medicine, JoVE presents an article from the University College London and the University of Oxford that investigates the physiological correlates of nociception, or pain sensation, in infants. Typically, infants respond to noxious stimuli by crying and changing their facial expressions - reactions considered to be autonomic and reliant on subcortical regions of the brain.
In order to understand the involvement of the cerebral cortex and spinal cord in infant nociception our authors first prepare infants for EEG and EMG recordings. They also set up physiological monitoring and video recording, so that the infant's facial expressions can be linked to physiological data. Following assessment of infant responses to neutral tactile stimuli, the baby is subjected to heel lancing - a clinical procedure used to obtain blood samples from infants. After data processing, electrical potentials can be compared between noxious and non-noxious stimuli in these infants, which will hopefully lead to meaningful conclusions, regarding how nociceptive information is interpreted by newborns.
JoVE's Bioengineering section takes a more clinical focus in December with an article from the University of Wisconsin, Madison, which involves the fabrication of a compartmentalized microfluidic device for studying cancer stem cell migration. Following device design, master molds are created by photolithography for the application of poly dimethyl siloxane (PDMS)- an organosilicon compound that is transparent and biologically inert - making it suitable for microfluidics applications with live cells. Specifically, our authors load tumor stem cells derived from multiform glioblastoma, a highly malignant brain cancer, into the compartmentalized devices and then place them in a specialized imaging system that acquires data over a few days. 
Time lapse video from these authors show that cancer stem cells are able to regulate their morphology in the size-constrained microfluidic channels much like they would in the interstitial space, and fluidic isolation made possible by chambers like these facilitates drug screening for potential therapeutics that can affect cancer stem cell migration.
Late on in the month, JoVE travels to France, where investigators from the Institute Pasteur and the Centre National de la Recherche Scientifique, illustrate a method for inducing expression of channel-rhodopsin, a light-activated ion channel, into the olfactory bulb of mice, as well as subsequent neuronal stimulation using a miniature light emitting diode (LED).
Our authors give detailed step-by-step instructions for preparing injection needles, and for precise injection of virus into the rostral migratory stream, a site of migration for adult born neuroblasts. Furthermore, they show how miniature LED stimulation devices are fabricated, calibrated, and implanted atop a cranial window prepared over the bulb. In particular, our authors are interested in the way that adult born neurons integrate into existing olfactory neuronal circuits, and they show that LED stimulation in awake behaving animals, can lead to activation of virally-tranduced neuroblasts in the olfactory bulb, which have migrated from the rostral migratory stream.
These video-articles comprise JoVE's highlights for the month of December. Other noteworthy upcoming articles illustrate methods for patterning conductive inks into microelectrodes, measuring cytosolic calcium in contractile lymphatics, and imaging neuronal responses to pheromones in the vomeronasal organ.

Here are some highlights from the December 2011 Issue of Journal of Visualized Experiments (JoVE).

목성이 December

The Journal of Visualized Experiments (JoVE) closes 2011 with a December issue that begins by honoring World's AIDS Day with an article that deals with ...

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생물학

October 2012: This Month in JoVE

Here are some highlights from the October 2012 issue of Journal of Visualized Experiments (JoVE). Gr&oslash;nlund et al. demonstrate how to isolate and analyze specific cell types in plant leaves expressing green fluorescent protein (GFP) using fluorescence-activated cell sorting (FACS). This method overcomes the interfering fluorescence of the chlorophyll found in leaves, and distinguishes GFP-expressing protoplasts from non-GFP protoplasts. In JoVE Neuroscience, Babona-Pilipos et al. demonstrate how to construct a chamber for measuring galvanotaxis, or cell migration within an electric field. Through time-lapse imaging and image analysis, the authors can study the migratory behavior of neural precursor cells in an electric field, which may lead to the use of electrical stimulation to direct neural precursors to sites of injury or disease. Articles involving microfluidic platforms have a significant publication history in JoVE. Harris et al. have released three articles, which involve a microfluidic device for separating axons from neuronal cell bodies. This month in JoVE Neuroscience, Higashimori et al. use this microfluidic platform to investigate the interactions between neuronal axons and glial cells, which are critical for the physiological function of the nervous system. In JoVE Bioengineering, two research groups demonstrate the novel bioadhesive properties of chitosan-a polymer derived from chitin, which is found in fungal cell walls or in the exoskeletons of crustaceans and insects. Chitosan is used in many industrial and agricultural applications, and bioengineers are also finding uses for it in surgical applications. Foster et al. have developed a laser-activated surgical film called Surgilux, which combines chitosan with indocyanine green ICG), a photosensitive dye. This surgical film binds strongly to tissue, such as muscle, after laser irradiation. Lauto et al. developed a surgical film that combines chitosan with the photoactive dye, rose Bengal. This novel film also binds strongly to tissues, such as intestine, after it is irradiated. These adhesive films are biocompatible and may potentially be used in various surgical procedures in place of sutures. In JoVE Clinical and Translational Medicine, Fiema et al. demonstrate a high-throughput technique for validating biomarkers in graft vs. host disease, a common and life-threatening complication of cell or tissue transplantation. The authors use commercially available ELISAs to analyze multiple proteins in sequential fashion. In JoVE Immunology and Infection, Keyel et al. demonstrate how to measure the real-time kinetics of immune cell responses to bacterial toxins using high-speed live cell microscopy. This method can be used to show how immune cells respond to bacterial toxins. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response. In JoVE Applied Physics, Borisenko et al. determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy in a synchotron radiation facility. By combining recent advances in synchotron radiation, surface science, and cryogenics, this method can gain a precise picture of the energy and momentum of electrons inside a solid, and address key questions in the field of condensed matter physics. This preview summarizes just a few of the notable video-articles available in the October 2012 issue of JoVE. For additional videos, please visit <a href="http://www.jove.com">www.jove.com</a>.

Here are some highlights from the October 2012 Issue of Journal of Visualized Experiments (JoVE).

2012년 10월 : 주피터에 이번 달

Here are some highlights from the October 2012 issue of Journal of Visualized Experiments (JoVE).  Gr&oslash;nlund et al. demonstrate how to isolate and ...

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

2012 : 검토 년

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...