<a href="https://www.jove.com/video/3406">일반 담배 연기에 가스 단계 무료 부수을 감지하고 청소를위한 프로토콜</a> 장기 사이 유 1, 보리스 G. Dzikovski 2, 잭 H. 해방 3, 2 1 CDCF - AOX 연구소, 고급 ESR 기술 (ACERT) 2 국립 바이오 메디컬 센터, 화학 및 화학 생물학과, 코넬 대학, 3 ACERT 연구, 화학의 센터 부와 화학 생물학, 베이커 연구소, 코넬 대학교 스핀 트래핑 ESR 분광법은 식물 항산화 리코핀, pycnogenol 및 담배 연기에 가스 단계 래디칼 소기의 포도씨 추출물의 효과를 연구하는 데 사용되었습니다. <a href="https://www.jove.com/video/3671">경비원이 심방 세동에 대한 심방 부위 요 폐쇄 장치를 왼쪽</a> 스벤 Möbius - 무선 인터넷 접속nkler, 마커스 Sandri, 노먼 Mangne​​r, 필립 루르 즈, Ingo Dähnert, 게르하르트 슐러 라이프 치히 심장 센터의 대학 함께 비디오 심방 부위 (LAA) 장치를 왼쪽 경비원의 percutaneous 게재 위치에 대한 절차를 설명합니다. 경비원이 영구적으로 주입, 또는 사람들이 thromboembolic 뇌졸중을 방지 LAA를 종료하기 전에 피를 잡는 왼쪽 심방 부위 (LAA)의 개통에 약간 말초하도록 설계 nitinol 장치입니다. <a href="https://www.jove.com/video/3413">운동 중 에너지 시스템의 공헌을 결정하는</a> Guilherme G. Artioli 1 Rômulo C. Bertuzzi 2, 해밀턴 Roschel 1, 3, 산드로 H. Mendes 1, 안토니오 H. Lancha 주니어 1, 에머슨 키니 사 응용 영양 1 연구소, 체육 교육 및 스포츠, Universi 대학상 파울로, 2 에어로빅 성능 연구 그룹, 체육 및 스포츠의 학교 타이, 상 파울로 대학, 강도 훈련에 신경 근육 적응 3 연구소, 체육 교육 및 스포츠, 상 파울로 대학 학부, 4 무술 및 전투 스포츠 연구 그룹, 체육 교육 및 스포츠 대학, 상 파울로 대학 이 프로토콜은 운동과 스포츠 과학에 초점을 맞춘 연구는 운동의 다양한 동안 총 에너지 지출에 대한 세 가지 다른 에너지 시스템의 상대적 기여를 확인할 수 있습니다. <a href="https://www.jove.com/video/3992">탄소 고정 잠재력의 화학적 성분은 층상 남극 호수 및 평가에서 미생물 진핵 세포 농축 문화의 확립</a> 제나 M. Dolhi, 니콜라스 Ketchum, 레이첼 M. 모건 - 키스 미생물학학과, 마이애미 취소iversity 미생물의 eukaryotes 모두 영구적으로 얼음으로 덮인 남극 호수에 photosynthetically 파생 탄소와 최고 육식 동물의 원천입니다. 이 보고서는 남극 호수, 호수 Bonney에서 metabolically 다양한 미생물 eukaryotes를 분리 할 수​​있는 농축 문화 접근 방식을 설명하고 Ribulose-1 ,5-bisphophate carboxylase의 oxygenase (RubisCO) 활동을 위해 방사성 동위 원소 분석을 사용하여 무기 탄소 고정의 잠재력을 평가합니다. <a href="https://www.jove.com/video/4191">실험실 규모의 합성 거미의 실크 생산</a> 양 Hsia, 에릭 Gnesa, 라이언 Pacheco, 크리스틴 Kohler, 펠리 시아 제프리, 크레이그 Vierra 생명 과학학과, 태평양 대학 거미 실크의 뛰어난 기계적 생화학 특성에도 불구하고,이 자료는 종래의 방법으로 대량으로 수확 할 수 없습니다. 여기 내림차순차세대 biomaterials으로 거미 실크 생산과 사용을 공부 수사의 중요한 과정입니다 인공 거미 실크 섬유를 회전 할 수있는 효율적인 전략을 리베. <a href="https://www.jove.com/video/3966">결정 토양을 전송 기생충 감염 현황 및 학교 세 어린이의 체육</a> 야프 1, 2, 토마스 FURST : 비르 1, 2, 이반 뮐러 1, 2, Susi Kriemler 1, 2, Jürg Utzinger 1, 2, 피터 Steinmann 1, 2 Peiling 한 역학의 부와 보건, 스위스 열대와 보건 연구소, 바젤, 스위스, 바젤 2 대학, 바젤, 스위스 토양 - 전송 helminths (STHs)과 만성 감염 stunting, 그리고 성장 자녀의 낭비, 흡수 장애를 일으 킵니다. 따라서, 이러한 감염은 또한 매가 솟아 오르는 상징을 줄일 것을 그럴듯어린이의 ICAL의 피트니스. 여기, 우리는 STHs과 어린이의 물리적 피트니스을 평가하기위한 20 미터 셔틀이 운영하는 시험의 진단을위한 두 기술을 표시합니다. <a href="https://www.jove.com/video/4427">클리닉에서 시뮬레이션 운전 : 비주얼 필드 결함으로 환자의 일상 생활 활동에서 Visual 탐색 행동 테스트</a> 요한나 Hamel 1, 2, Antje 크래프트 1, 스벤 Ohl 3, 소피 드 Beukelaer 1, 하인리히 J. Audebert 1, 2, 스테판 A. 브랜트 1 신경과 1 부 Universitätsmedizin Charité, 뇌졸중 연구 베를린 2 센터 (CSB), Universitätsmedizin Charité, 3 베를린 마음의 학교와 뇌, 험 볼트 Universität zu 베를린 가능성이 가장 높은 변수로 인해 일상 생활에 여러 제약에 대한 행정보고 한 후 시각적 결손이있는 환자임상 루틴에서 구별하기 어렵 보상 전략. 우리는 할 수 있습니다 다른 보상 머리와 눈 운동 - 전략의 측정과 성능을 운전에 미치는 영향을 평가.에게 임상 설정을 제시 <a href="https://www.jove.com/video/4227">라이브 세포 형광 현미경을 사용하여 박테리아 독소 유도 응답의 시각화</a> 피터 A. Keyel 1, 미셸 E. 점령 한, 사이먼 C. 왓킨스 2, 러셀 D. 제염업자 1 면역학의 1 부, 피츠버그 대학 의과 대학, 세포 생물학의 2 부와 생리학, 의학 피츠버그 대학 학부 재조합 E.에서 콜레스테롤 바인딩 독소 streptolysin O를 정화하기위한 방법 대장균 및 진핵 세포를 사는 바인딩 독소 시각화가 설명되어 있습니다. 독소의 현지화 전달 전독소 생물학의 소설 측면을 드러내는 대상 셀에 신속하고 복잡한 변경 사항을 nduces. <a href="https://www.jove.com/video/50129">울트라 낮은 온도에서 각도 - 해결 Photoemission 분광법</a> 세르게이 V. Borisenko 1 볼로디미르 B. Zabolotnyy 1, 알렉산더 A. Kordyuk 1, 2, 다닐 V. Evtushinsky 1, 티무르 K. 김 1, 3, Emanuela Carleschi 4, 브라이언 P. 도일 4, Rosalba Fittipaldi 5, 마리오 Cuoco 5, 안토니오 Vecchione 5, 헬무트 버거 6 솔리드 스테이트 연구 1 연구소, IFW-드레스덴, 우크라이나의 과학 국립 아카데미, 3 다이아몬드 광원 LTD, 물리 4 부, 요하네스 버그 대학교, 5 CNR-스핀, 그리고 Dipartimento 디 Fisica &quot;ER의 금속 물리 2 연구소 Caianiello &quot;, Università 디 살레르노, 6 연구소복잡한 물질, 가르 Polytechnique Fédérale 드 로잔 (Lausanne)의 물리 이 방법의 전반적인 목표는 싱크로트론 방사와 각도 - 해결 Photoemission 분광법을 사용하여 매우 낮은 온도에서 고체의 낮은 에너지 전자 구조를 결정하는 것입니다. <a href="https://www.jove.com/video/50027">노래하는 새는에 청각 피드백을 조작하기위한 경량, 헤드폰 기반의 시스템</a> 루카스 A. 호프만, 1, 2, 코너 W. 켈리 1, 3, 데이비드 A. 니콜슨, 1, 2, 사무엘 J. 소버 1 생물 1 부,에 모리 대학, 2 신경 과학 대학원 프로그램,에 모리 대학 신경 과학 및 행동 생물학 3 프로그램,에 모리 대학교 우리는 가수의 자연 청각 피드백을 대체하기에 적합 소형화 헤드폰의 디자인과 조립을 설명조작 음향 신호. 온라인 사운드 처리 하드웨어, 노래 출력을 조작하는 헤드폰을 통해 청각의 의견에 실시간으로 오류를 소개하고, 보컬 모터 학습을 유도하는 데 사용됩니다. <a href="https://www.jove.com/video/50179">꿀벌에 촉각 시설과 Antennal 샘플링 전략의 움직임 분석 (API를 mellifera L.)</a> 사미르 Mujagić, 사이먼 마이클 Würth, 스벤 Hellbach, Volker Dürr 생물 인공 이식, CITEC -인지 상호 작용 기술 - 우수의 센터 빌레펠트 대학 이 프로토콜에서 우리는 상태로 촉각 자극에 꿀벌을 밝혀와 고급 규모의 antennal 샘플링 패턴의 운동학을 분석하는 차원 모션 캡처 기술을 소개하는 방법을 보여줍니다.

관심 없음 충돌이 선언 없습니다.

2012: a year in review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Watch this Scientific Journal Video about 2012: A Year In Review at JoVE.com

2012: A Year In Review

여기 시각화 실험 논문집 (주피터)에서 2012 년의 이정표와 하이라이트의 일부를 살펴입니다.

2012 : 검토 년

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...

Research

JoVE Journal

Biology

11.5K 조회수.  Massachusetts Eye and Ear. 여기 시각화 실험 논문집 (주피터)에서 2012 년의 이정표와 하이라이트의 일부를 살펴입니다.

비디오: 2012: A Year In Review

February 2012: This Month in JoVE

The February issue of the Journal of Visualized Experiments (JoVE) takes a clinical theme with the release of two articles, which demonstrate implantations of medical devices used to treat cardiac arrhythmia. More specifically, both articles focus on treating atrial fibrillation - a devastating condition that can ultimately lead to stroke.
The first procedure, led by Professor Dietmar Baensch from the University Hospital Rostock, involves the surgical implantation of an implantable cardioverter-defibrillator, or ICD. ICDs are devices widely used for the termination of ventricular arrhythmias. However, if the site of abnormal pacemaking is supraventricular in origin, such as in the left atrium, these devices can deliver inappropriate and painful shocks. To overcome this issue with potential misdiagnosis, as well as higher complication rates observed with dual chamber elecectrodes, Biotronik has developed a single ICD lead, which is capable of both atrial and ventricular sensing. Guided by X-ray fluoroscopy and electrophysiology, Dr. Baensch and his team demonstrate the surgical procedure for inserting, testing, and anchoring the single chamber electrode. Following electrode positioning, the Lumax defibrillator is joined to the electrode and inserted into the patient. To assess device function ventricular fibrillation is induced via a T-wave shock, and the ability of the defibrillator to sense arrhythmia and administer a corrective shock to is assessed.
While the Lumax ICD system can attenuate cardiac arrhythmia by correcting cardiac pacemaking, the WATCHMAN left atrial appendage (LAA) occlusion device from Boston Scientific is designed to address a severe complication with atrial fibrillation - thromboembolic stroke. In patients with atrial fibrillation, the left atrial appendage, a muscular pouch in the left atrium, can act as a reservoir for clotting blood, which if dislodged, can form emboli that can cause stroke. When implanted, the WATCHMAN device can trap clots within the LAA before they exit. Following assessment of LAA morphology, our authors, led by Dr. Sven M&ouml;bius-Winkler from the University of Leipzig, demonstrate methods for inserting a guidewire through the femoral artery into the heart, advancing the device into the left atrium, and deployment of the device in the left atrial appendage. Studies have shown that patients receiving WATCHMAN intervention have a reduced probability of adverse cardiac events when compared to patients given the anticoagulant medication warfarin.
While both the Lumax ICD system and the WATCHMAN occlusion device show promise for treating cardiac arrhythmia and its complications, neither device is approved for use in the US. Through video-publication in JoVE, our authors have provided a resource for US physicians to view these sensitive device implantation procedures before they become available across the Atlantic.
One consequence of thromboembolism is hypoxia, and JoVE travels to the University of Rochester to document a method for 2-photon imaging of microregional hypoxia in the cerebral cortex. Our authors illustrate a detailed open cranial window preparation followed by 2-photon imaging of endogenous nicotinamide adenine dinucleotide, or NADH, fluorescence. NADH is well known for its ability to transport electrons during cellular respiration, and it just so happens that NADH fluorescence exhibits a nonlinear relationship with the partial pressure of oxygen in tissues. In the cortex, NADH fluorescence increases as hypoxia is induced, allowing these investigators to determine which cortical areas are most vulnerable to hypoxia, as well as the geometrical pattern of oxygen diffusion from vessels.
For Bioengineering, JoVE heads to Wayne State University to capture a procedure for visualizing the breast cancer tumor microenvironment, in vitro. Our authors demonstrate a procedure for the assembly of a breast cancer co-culture system, which they refer to as Mammary Architecture and Microenvironment Engineering, or MAME. Briefly, these culture models consist of a layer of human breast fibroblasts in collagen 1 matrix, below a layer of reconstituted basement membrane matrix, upon which ductal carcinoma cells from the breast are added before the entire model is submerged in medium. When imaged in 4D, or in 3 dimensions over time, the proliferation of carcinoma cells can be tracked. In addition, degradation of the basement membrane can be assessed as well, thanks to the incorporation of a type of collagen that fluoresces when hydrolyzed. This innovative culture system can provide insight for breast cancer researchers into the proteolytic pathways that promote invasive phenotypes in breast cancer cells and can be used to screen for new small molecule inhibitors that may hinder tumor proliferation.
In Immunology and Infection, JoVE shifts its focus to vector borne disease, where investigators from the Center for Disease control in Fort Collins, Colorado, present three fundamentally important procedures for understanding how infectious pathogens migrate through the tick. The first procedure involves removing one of the legs from the tick, which enables the collection of hemolymph - the fluid carries oxygen to the tick's organs. The bacteria that cause Lyme Disease, Borrelia burgdorferi, will move to the hemolymph of the tick once a bloodmeal has been absorbed by the midgut. The second dissection protocol illustrates steps for accessing the hemocoel and removing the salivary glands, which is where Borrrelia reside before transmission to a host. The final protocol begins with application of the muscarinic agonist, pilocarpine, to the dorsal surface of an engorged tick, in order to induce salivation. Saliva is collected from a mouthpart referred to as the hypostome and manual techniques for promoting tick salivation via gentle massage, are demonstrated.
From vector borne disease to medical device implantation, February is brimming with content submitted from authors dedicated to understanding and treating human disease. Other notable articles for the month of February include methods for assessing the metastatic potential of ovarian cancer, spatial analysis of neuropeptides in a rodent model of Parkinson's, and measurements of ventricular pressure in neonatal and embryonic mice. From bench to bedside, no other journal provides a window into biomedical research quite like JoVE. Stay tuned for February's content!

Here are some highlights from the February 2012 Issue of Journal of Visualized Experiments (JoVE).

2012년 2월 : 주피터에 이번 달

The February issue of the Journal of Visualized Experiments (JoVE) takes a clinical theme with the release of two articles, which demonstrate ...

연구

생물학

August 2012: This Month in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens free lens-free "on-chip" microscopy platform, which they have pioneered.
Back in 2009, the Ozcan lab published their first article, which demonstrated the use of a lens-free imaging platform that could image cells within whole blood. This imaging technique involves placing a sample directly on an optoelectric sensor array, like the CCD or CMOS chips found in the standard camcorder. Then, LED illumination from above the sample casts shadows on the chip, which are recorded as digital holograms that can be subjected to image processing. Not only does this lensless imaging system allow for an extremely large field of view (FOV of 0.6-8 cm2), the lack of bulky optical components makes it excellent for microfluidic applications. The Ozcan group demonstrates this in their follow-up 2011 JoVE article, in which fluorescently labeled white blood cells are imaged within a microfluidic chip.
This August in JoVE, the Ozcan group demonstrates further advancements to their lens-free microscopy platform by applying it to tomographic imaging of the nematode, C. elegans. By changing the angle of illumination, using a motorized rotation stage, the Ozcan group shows how multiple holographic slices may be generated for a sample-which, following processing, can be combined into tomograms. The Ozcan group confirms that their 3D reconstruction of the worm is successful by citing that the pharyngeal tube, a 5-micron-thick structure, is only visible in a single slice and in a 3D reconstructed cross section.
The compact and inexpensive nature of the lens-free imaging systems - designed and assembled by the Ozcan group - make them well suited for point-of-care diagnostic applications, or even cell phone based microscopy that can be performed in the field.
At the Ernest Gallo Clinic and Research Center at University of California, San Francisco JoVE learns how to visualize morphological changes in single cells using three-dimensional fluorescence microscopy and computer analysis. In this procedure, cells are given agonist, antagonist plus agonist, or no treatment, and prepared for immunofluorescence microscopy. Three-dimensional images are acquired with the help of a two-photon laser system, and the fluorescence data are processed on the computer using Imaris and Matlab.
To demonstrate the power of this technique, our authors quantify single-cell phenotypic changes in transfected HEK293 cells. Three-dimensional morphometric analysis shows dramatic changes in cells treated with agonist. These changes are not always apparent when using conventional 2D analysis. This technique allows morphological changes in single cells to be quantified and characterized in response to pharmacologic stimuli, offering a powerful new assessment tool to the arsenal of drug discovery research.
This month, in JoVE Applied Physics, we travel to South Carolina to visit the School of Materials Science and Engineering at Clemson University. Here, researchers show us how to construct lithium batteries for laboratory research.
The basic components of a lithium coin cell are the working electrode, the lithium electrolyte, and a lithium foil counter electrode. The different components of the coin cell are carefully assembled and sealed with a crimping machine. The finished coin cell can then be characterized in a battery tester. Using their homemade coin cells, our researchers can experiment with using different materials to make the cathodes of lithium coin cells.
This month in JoVE Immunology and Infection, we head to Basel, Switzerland to witness two techniques for diagnosing helminth infections. We also see how investigators perform the shuttle run test in children, in order to assess the effects of these infections on physical fitness.
Helminths are parasitic worms that can live inside humans and animals. Many species infect the intestinal tract, and may be transmitted through soil. Our authors demonstrate the Kato-Katz technique, which is used for diagnosing several common soil-transmitted helminths. They also demonstrate the Baermann technique, which uses light to stimulate the migration of certain types of phototactic helminths out of sample preparations. The various types of helminth species can subsequently be determined based on morphology.
Our authors then demonstrate how to set up and perform a 20-meter shuttle run test for children, and to measure the impact of helminth infections on physical fitness. This method can help determine the true burden of soil-transmitted helminth infections on overall health.
This brief summary highlights but a few notable video-articles that will be released in August. We'll also feature articles that demonstrate how to express genes in the midbrain of the chick embryo, study neutrophil transmigration in parallel flow chambers lined with endothelial cells, and precisely measure proteolytic degradation of the extracellular matrix.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens-free "on-chip" microscopy platform, which they have pioneered.

2012년 8월 : ​​주피터의 이번 달

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and ...

October 2012: This Month in JoVE

Here are some highlights from the October 2012 issue of Journal of Visualized Experiments (JoVE). Gr&oslash;nlund et al. demonstrate how to isolate and analyze specific cell types in plant leaves expressing green fluorescent protein (GFP) using fluorescence-activated cell sorting (FACS). This method overcomes the interfering fluorescence of the chlorophyll found in leaves, and distinguishes GFP-expressing protoplasts from non-GFP protoplasts. In JoVE Neuroscience, Babona-Pilipos et al. demonstrate how to construct a chamber for measuring galvanotaxis, or cell migration within an electric field. Through time-lapse imaging and image analysis, the authors can study the migratory behavior of neural precursor cells in an electric field, which may lead to the use of electrical stimulation to direct neural precursors to sites of injury or disease. Articles involving microfluidic platforms have a significant publication history in JoVE. Harris et al. have released three articles, which involve a microfluidic device for separating axons from neuronal cell bodies. This month in JoVE Neuroscience, Higashimori et al. use this microfluidic platform to investigate the interactions between neuronal axons and glial cells, which are critical for the physiological function of the nervous system. In JoVE Bioengineering, two research groups demonstrate the novel bioadhesive properties of chitosan-a polymer derived from chitin, which is found in fungal cell walls or in the exoskeletons of crustaceans and insects. Chitosan is used in many industrial and agricultural applications, and bioengineers are also finding uses for it in surgical applications. Foster et al. have developed a laser-activated surgical film called Surgilux, which combines chitosan with indocyanine green ICG), a photosensitive dye. This surgical film binds strongly to tissue, such as muscle, after laser irradiation. Lauto et al. developed a surgical film that combines chitosan with the photoactive dye, rose Bengal. This novel film also binds strongly to tissues, such as intestine, after it is irradiated. These adhesive films are biocompatible and may potentially be used in various surgical procedures in place of sutures. In JoVE Clinical and Translational Medicine, Fiema et al. demonstrate a high-throughput technique for validating biomarkers in graft vs. host disease, a common and life-threatening complication of cell or tissue transplantation. The authors use commercially available ELISAs to analyze multiple proteins in sequential fashion. In JoVE Immunology and Infection, Keyel et al. demonstrate how to measure the real-time kinetics of immune cell responses to bacterial toxins using high-speed live cell microscopy. This method can be used to show how immune cells respond to bacterial toxins. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response. In JoVE Applied Physics, Borisenko et al. determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy in a synchotron radiation facility. By combining recent advances in synchotron radiation, surface science, and cryogenics, this method can gain a precise picture of the energy and momentum of electrons inside a solid, and address key questions in the field of condensed matter physics. This preview summarizes just a few of the notable video-articles available in the October 2012 issue of JoVE. For additional videos, please visit <a href="http://www.jove.com">www.jove.com</a>.

Here are some highlights from the October 2012 Issue of Journal of Visualized Experiments (JoVE).

2012년 10월 : 주피터에 이번 달

Here are some highlights from the October 2012 issue of Journal of Visualized Experiments (JoVE).  Gr&oslash;nlund et al. demonstrate how to isolate and ...