JoVE Logo
교수 리소스 센터

로그인

Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

DOI :

10.3791/54981-v

February 10th, 2017

February 10th, 2017

9,143 Views

1Division of Biology and Biological Engineering, California Institute of Technology (Caltech)

In Parkinson's Disease (PD), Substantia Nigra (SNc) dopaminergic neurons degenerate, leading to motor dysfunction. Here we report a protocol for culturing ventral midbrain neurons from a mouse expressing eGFP driven by a Tyrosine Hydroxylase (TH) promoter sequence, harvesting individual fluorescent neurons from the cultures, and measuring their transcriptome using RNA-seq.

Tags

Keywords RNA Sequencing

-- Views

Related Videos

article

Application of a NMDA Receptor Conductance in Rat Midbrain Dopaminergic Neurons Using the Dynamic Clamp Technique

article

Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro

article

In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development

article

In ovo Expression of MicroRNA in Ventral Chick Midbrain

article

Assessing Neurodegenerative Phenotypes in Drosophila Dopaminergic Neurons by Climbing Assays and Whole Brain Immunostaining

article

Primary Culture of Mouse Dopaminergic Neurons

article

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

article

External Excitation of Neurons Using Electric and Magnetic Fields in One- and Two-dimensional Cultures

article

Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation

article

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing (DIVA-Seq)

JoVE Logo

개인 정보 보호

이용 약관

정책

연구

교육

JoVE 소개

Copyright © 2024 MyJoVE Corporation. 판권 소유