Metabolic Glycoengineering of Sialic Acid Using N-acyl-modified Mannosamines

1:02

Synthesis of ManNProp and Related N-acyl-modefied Mannosamines

2:26

Cell Culture and Cell Lysis for HPLC Analysis

4:28

Separation of the Membrane Fraction and Acid Hydrolysis

5:27

Fluorescent Labeling, HPLC, and Mass Spetrometry Analysis

6:55

Western Blot Analysis of Polysialic Acid in Kelly Neuroblastoma Cells

9:00

Results: Modulating Expression of Sialic Acid on Cell Surfaces Using N-Side Chain Modified Mannosamines

10:40

Conclusion