Extraction and Processing of Brain Tissue from Sprague-Dawley Rats
2:59
Centrifugation
4:27
Results: Analysis of Freshly Isolated Rat Brain Microvessel Samples
6:07
Conclusion
필기록
The overall goal of this procedure is to isolate cerebral microvessels from rat brain for biochemical studies aimed at understanding molecular characteristics of the mammalian blood-brain barrier. This method can help to answer key questions in bl
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Here, a method for isolation of rat brain microvessels and for the preparation of membrane samples is described. This protocol has the clear advantage of producing enriched microvessel samples with acceptable protein yield from individual animals. Samples can then be used for robust protein analyses at the brain microvascular endothelium.