JoVE Logo
교수 리소스 센터

로그인

Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts

DOI :

10.3791/58240-v

September 27th, 2018

September 27th, 2018

7,975 Views

1Department of Biophysics, University of Michigan, Ann Arbor, 2Department of Chemistry, University of Michigan, Ann Arbor, 3Department of Computer Science, Wayne State University, 4Department of Physics, University of Michigan, Ann Arbor

We present a method for the generation of in vitro self-sustained mitotic oscillations at the single-cell level by encapsulating egg extracts of Xenopus laevis in water-in-oil microemulsions.

Tags

Keywords Cell cycle Oscillations

-- Views

Related Videos

article

Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones

article

Voltage-clamp Fluorometry in Xenopus Oocytes Using Fluorescent Unnatural Amino Acids

article

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

article

Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus

article

In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers

article

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

article

Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins

article

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli

article

Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription

article

Reconstitution of Msp1 Extraction Activity with Fully Purified Components

JoVE Logo

개인 정보 보호

이용 약관

정책

연구

교육

JoVE 소개

Copyright © 2024 MyJoVE Corporation. 판권 소유