April 16th, 2020
•This protocol describes the robust generation of macrophages from human induced pluripotent stem cells, and methods for their subsequent characterization. Cell surface marker expression, gene expression, and functional assays are used to assess the phenotype and function of these iPSC-derived macrophages.
Related Videos
Directed Differentiation of Induced Pluripotent Stem Cells towards T Lymphocytes
Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine
Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells
Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)
Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity
Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry
SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells
Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages
Neisseria meningitidis Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells
Copyright © 2024 MyJoVE Corporation. 판권 소유