June 29th, 2021
•This protocol describes the extraction and visualization of aggregated and soluble proteins from Escherichia coli after treatment with a proteotoxic antimicrobial. Following this procedure allows a qualitative comparison of protein aggregate formation in vivo in different bacterial strains and/or between treatments.
Related Videos
Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification
Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
Enrichment of Detergent-insoluble Protein Aggregates from Human Postmortem Brain
Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli
Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli
Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli
Copyright © 2024 MyJoVE Corporation. 판권 소유