National Yang Ming Chiao Tung University

4 ARTICLES PUBLISHED IN JoVE

Medicine

Ex Vivo and In Vivo Animal Models for Mechanical and Chemical Injuries of Corneal Epithelium

¹Department of Ophthalmology, Chang-Gung Memorial Hospital, ²Chang-Gung University College of Medicine, ³Institute of Clinical Medicine, National Yang Ming Chiao Tung University

Here, animal models based on mouse and rabbit are developed for mechanical and chemical injury of corneal epithelium to screen new therapeutics and the underlying mechanism.

Medicine

Production of Modified Autologous Conditioned Serum and Ex Vivo Assessment of Its Healing Potential in Murine Corneal Epithelium

Chun Hsiung^*1,2, Yu-Ting Liu^*1,2, Chen-Ying Su³, Chung-Chuan Hsiung^4,5, Kuo-Hsuan Hung^2,6,7, Lung-Kun Yeh^2,6

¹Department of Education, Chang Gung Memorial Hospital Linkou, ²School of Medicine, Chang Gung University, ³Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, ⁴Cell Therapy Center, Tri-Service General Hospital, ⁵Department of General Surgery, Bo-Ai Clinic, ⁶Department of Ophthalmology, Chang Gung Memorial Hospital Linkou, ⁷Institute of Clinical Medicine, National Yang Ming Chiao Tung University

This article describes a protocol to simplify the process and render the preparation of autologous conditioned serum (ACS) less expensive. No special syringes or surface-coated glass beads are needed. Moreover, the modified ACS (mACS) has competitive advantages over conventional autologous serum in the corneal wound healing of murine eyes ex vivo.

Neuroscience

Development of an Uncomplicated Mild Traumatic Brain Injury Model Modified by Weight-Drop Method and Evidenced by Magnetic Resonance Imaging

Pin-Hui Kuo¹, Tzu-Hsuan Tang¹, Shu-Hui Huang¹, Bao-Yu Hsieh^2,3, Chia-Feng Lu¹, Yu-Chieh Jill Kao¹

¹Department of Biomedical Imaging and Radiological Sciences, National Yang Ming Chiao Tung University, ²Department of Medical Imaging and Radiological Sciences, College of Medicine, Chang Gung University, ³Department of Medical Imaging and Intervention, Chang Gung Memorial Hospital at Linkou

Here, we present a protocol to establish a closed-head injury animal model replicating the neuroimage outcome of uncomplicated mild traumatic brain injury with the preserved brain structure in the acute phase and long-term brain atrophy. Longitudinal magnetic resonance imaging is the primary method used for evidence.

Medicine

Laparoscopic-Assisted Seldinger Technique for Peritoneal Dialysis Catheter Insertion

Cheng-Yen Chen^1,2, Ming-Tsun Tsai^2,3, Niang-Cheng Lin^1,2, Chih-Ching Lin^2,3, Szu-Yuan Li^2,3

¹Division of Transplantation Surgery, Department of Surgery, Taipei Veterans General Hospital, ²School of Medicine, National Yang Ming Chiao Tung University, ³Division of Nephrology, Department of Medicine, Taipei Veterans General Hospital

Here, we present a protocol for performing the laparoscopic-assisted Seldinger technique (LAST) for peritoneal dialysis (PD) catheter insertion, highlighting a combination of the Seldinger method's minimal invasiveness with the enhanced visualization of laparoscopy.