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National Yang Ming Chiao Tung University

2 ARTICLES PUBLISHED IN JoVE

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Medicine

Ex Vivo and In Vivo Animal Models for Mechanical and Chemical Injuries of Corneal Epithelium
Kuo Hsuan Hung 1,2,3, Lung Kun Yeh 1,2
1Department of Ophthalmology, Chang-Gung Memorial Hospital, 2Chang-Gung University College of Medicine, 3Institute of Clinical Medicine, National Yang Ming Chiao Tung University

Here, animal models based on mouse and rabbit are developed for mechanical and chemical injury of corneal epithelium to screen new therapeutics and the underlying mechanism.

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Medicine

Production of Modified Autologous Conditioned Serum and Ex Vivo Assessment of Its Healing Potential in Murine Corneal Epithelium
Chun Hsiung *1,2, Yu-Ting Liu *1,2, Chen-Ying Su 3, Chung-Chuan Hsiung 4,5, Kuo-Hsuan Hung 2,6,7, Lung-Kun Yeh 2,6
1Department of Education, Chang Gung Memorial Hospital Linkou, 2School of Medicine, Chang Gung University, 3Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, 4Cell Therapy Center, Tri-Service General Hospital, 5Department of General Surgery, Bo-Ai Clinic, 6Department of Ophthalmology, Chang Gung Memorial Hospital Linkou, 7Institute of Clinical Medicine, National Yang Ming Chiao Tung University

This article describes a protocol to simplify the process and render the preparation of autologous conditioned serum (ACS) less expensive. No special syringes or surface-coated glass beads are needed. Moreover, the modified ACS (mACS) has competitive advantages over conventional autologous serum in the corneal wound healing of murine eyes ex vivo.

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