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National Yang Ming Chiao Tung University

2 ARTICLES PUBLISHED IN JoVE

Medicine

Ex Vivo and In Vivo Animal Models for Mechanical and Chemical Injuries of Corneal Epithelium

¹Department of Ophthalmology, Chang-Gung Memorial Hospital, ²Chang-Gung University College of Medicine, ³Institute of Clinical Medicine, National Yang Ming Chiao Tung University

Here, animal models based on mouse and rabbit are developed for mechanical and chemical injury of corneal epithelium to screen new therapeutics and the underlying mechanism.

Medicine

Production of Modified Autologous Conditioned Serum and Ex Vivo Assessment of Its Healing Potential in Murine Corneal Epithelium

Chun Hsiung^*1,2, Yu-Ting Liu^*1,2, Chen-Ying Su³, Chung-Chuan Hsiung^4,5, Kuo-Hsuan Hung^2,6,7, Lung-Kun Yeh^2,6

¹Department of Education, Chang Gung Memorial Hospital Linkou, ²School of Medicine, Chang Gung University, ³Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, ⁴Cell Therapy Center, Tri-Service General Hospital, ⁵Department of General Surgery, Bo-Ai Clinic, ⁶Department of Ophthalmology, Chang Gung Memorial Hospital Linkou, ⁷Institute of Clinical Medicine, National Yang Ming Chiao Tung University

This article describes a protocol to simplify the process and render the preparation of autologous conditioned serum (ACS) less expensive. No special syringes or surface-coated glass beads are needed. Moreover, the modified ACS (mACS) has competitive advantages over conventional autologous serum in the corneal wound healing of murine eyes ex vivo.