The authors&#39; research work was supported by the National Heart, Lung, and Blood Institute of the National Institutes of Health under award numbers R01HL133723 and R01HL139748 and the American Heart Association SFRN in Vascular Disease (18SFRN33960001). H.S. is supported by an AHA postdoctoral fellowship (18POST33990468). J.C. is supported by NCATS UL1TR001998. The content in this manuscript is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Ultrasound imaging in mice is performed with approval of the University of Kentucky Institutional Animal Care and Use Committee (IACUC protocol number: 2018-2967). During the imaging, the mice are anesthetized using isoflurane 1%&#8211;3% vol/vol and placed on a heating platform to reduce procedural stress and prevent hypothermia. Eye lubricant is applied to prevent corneal damage due to the loss of the blink reflex during anesthesia.
1. Equipment Setup
<ol>
	<li>Turn on the ultrasound machine, heating platform, and gel warmer (Figure 1).</li>
	<li>Open the ultrasound program. Enter the study information, such as the study name and mouse information.</li>
	<li>Check the isoflurane vaporizer and O2 tank. If the content is low, fill the isoflurane vaporizer and/or exchange it for a new O2 tank.</li>
	<li>Connect the anesthetic scavenging filters to the induction chamber and the nose cone.</li>
	<li>Open the branch for the induction chamber.</li>
	<li>Turn on the O2 tank.</li>
	<li>Turn the O2 and isoflurane knobs on the anesthesia vaporizer to 1 L/min and 0% vol/vol, respectively, to fill the chamber with O2.</li>
</ol>
2. Preparation of the Mouse
<ol>
	<li>Place the mouse in the O2-filled induction chamber to minimize unwanted cardiovascular changes due to anesthesia.</li>
	<li>Turn on the isoflurane vaporizer (1.5%&#8211;2.5 % vol/vol).</li>
	<li>Confirm the absence of the hind limb withdrawal reflex.</li>
	<li>Remove the mouse from the chamber and place one drop of sterile ophthalmic lubricant in each eye.</li>
	<li>Redirect the anesthesia to the nose cone and close the flow to the induction chamber.</li>
	<li>Lay the mouse dorsally on the heating platform with its nose in the anesthesia nose cone.</li>
	<li>Apply depilatory cream to the chest or abdomen, using a cotton swab. Minimize the amount of depilatory cream use to avoid irritation.</li>
	<li>Wait for 1 min, and then, gently wipe off all cream and hair.</li>
	<li>Irrigate the area with warm water and wipe it dry to completely remove the cream.</li>
	<li>Dot gel on each of the four copper leads on the platform.</li>
	<li>Tape each paw pad down (palms down) to the leads for electrocardiogram (ECG) readings. This will provide the ECG and respiratory physiology of the mouse while anesthetized.</li>
	<li>Verify that the heart rate is between 450&#8211;550 beats/min. Since anesthesia affects cardiac function, which can alter the aortic diameter, adjust the delivery rate of anesthesia so that the heart rate is in an appropriate range.</li>
	<li>Apply prewarmed ultrasonic gel to the prepared site.</li>
	<li>Attach the probe to the holder.</li>
	<li>Rotate the platform for optimal scanning and lower the probe until it is in contact with the ultrasonic gel.</li>
</ol>
3. Imaging of the Thoracic Aorta
<ol>
	<li>Tilt down the platform to the left side of the mouse.</li>
	<li>Put the probe on the right edge of the mouse&#39;s sternum (Figure 2A). Orient the reference marker on the probe caudally. 
	NOTE: The reference marker on the probe indicates the probe direction and is consistent with the maker on the monitor of the ultrasound system (Figure 2A-D). The shape of the marker varies in each ultrasound system.</li>
	<li>Use color Doppler on the thoracic aorta to confirm blood flow.</li>
	<li>Adjust the stage and probe angle to show the aorta clearly (Figure 3A,B). 
	NOTE: The aortic valve and innominate and pulmonary arteries can be used for anatomical landmarks for the right parasternal long axis view. Therefore, aortic images from this view can include the aortic valve and innominate and pulmonary arteries in one frame (Figure 3A). If it is difficult to capture the entire ascending aorta in one scan, due to aortic pathologies such as aortic dilation and tortuosity, the images should be captured separately. Since separated images have the potential to cause an underestimation of the aortic measurements, fine positioning of the stage and probe is required. The right parasternal long axis view is optimal for imaging the entire ascending aorta (Figure 3C). However, it is often difficult to capture the aortic sinus in this view, especially in aneurysmal aortas. The left parasternal long axis view enables a capture from the aortic root to the proximal ascending aorta as an alternative approach, although this view cannot capture the aortic arch in one frame (Figure 3C). For the left parasternal long axis view, put the probe on the left edge of the sternum (Figure 2B). The stage is flat or slightly tilted to the mouse&#39;s right. Carry out the other steps of the procedure in the same manner as the right parasternal long axis view. Advantages and disadvantages of these probe positions are described in Table 2. Aortic images must be captured consistently in either right or left parasternal long axis view.</li>
	<li>Crop the ultrasound image to increase the frame rate, using the knobs for image depth and width.</li>
	<li>Change the focal depth on the dorsal side of the ascending aorta, using the knob for focal depth.</li>
	<li>Verify the ultrasound parameters. The ultrasound settings for this protocol are described in Table 1.</li>
	<li>Move the probe gently, using the X- and Y-axis stage knob, to capture the longitudinal aortic image with the largest possible diameter.</li>
	<li>Store one cine loop.</li>
</ol>
4. Imaging of the Abdominal Aorta
<ol>
	<li>Place the probe transversely, just below the sternum and xiphoid process (Figure 2C). The reference marker on the probe should face the mouse&#39;s right side. The abdominal aorta should be located next to the inferior vena cava and/or portal vein (Figure 3D).</li>
	<li>Visualize the abdominal aorta with color Doppler to confirm pulsatile flow. 
	NOTE: If the Doppler angle is perpendicular to the blood flow, a color Doppler signal will not appear in the aorta. In addition to color Doppler imaging, the abdominal aorta can be distinguished from the vena cava and portal vein by slightly pressing down the probe. The vena cava and portal vein are compressible, while the aorta maintains its patency.</li>
	<li>Crop the ultrasound image to increase the frame rate.</li>
	<li>Change the focal depth to the posterior wall of the abdominal aorta.</li>
	<li>Move the probe caudally to visualize the branch points of the celiac and superior mesenteric arteries.</li>
	<li>Locate the right renal artery and use it as a landmark. 
	NOTE: Since abdominal aortic aneurysms may lead to aortic tortuosity, adjust the probe angle to image the abdominal aorta perpendicularly. For an internal control, one image of the right renal branch point should be captured.</li>
	<li>Capture a cine loop of the region of interest which shows the maximum dilation in the abdominal aorta (Figure 3D,E). 
	NOTE: The localization of aortic aneurysms varies in each animal model. Aortic dilation in angiotensin II-induced mice occurs predominantly in the suprarenal aorta, while CaCl2 or elastase induces aortic aneurysm in the infrarenal aortic in mice.</li>
</ol>
5. Postscanning Mouse Care and Cleanup
<ol>
	<li>Wipe off the ultrasonic gel, irrigate the chest or abdomen with warm water, and gently wipe the mouse dry.</li>
	<li>Return the mouse to its cage, which is placed on a heating pad.</li>
	<li>Turn off the isoflurane vaporizer and O2 tank. Refill the vaporizer if the isoflurane level is low.</li>
	<li>Clean the ultrasound machine, probe, and platform with a soft cloth and isopropyl alcohol or glutaraldehyde wipes.</li>
	<li>Download all files collected during the scan.</li>
	<li>Turn off the ultrasound machine.</li>
	<li>Return the mice to animal housing rooms after they have recovered from the anesthesia.</li>
</ol>
6. Analysis
<ol>
	<li>Analysis of thoracic aortic images
	<ol>
		<li>Launch the analysis software and open the ultrasound data. An example image of analysis software (Vevo LAB 3.0.0) is shown in Supplemental Figure 1.</li>
		<li>Select one aortic ultrasound image for measurements from the cine loop (Figure 4A,C,E,G and Supplemental Figure 1). 
		NOTE: This protocol typically detects six to seven heartbeats in one cine loop. Since the aortic diameter is different between systole and diastole (Figure 4A-G), the measurements need to be examined at a consistent phase of the cardiac cycle. Systole is defined from the R wave to the end of the T wave. In general, T waves are difficult to identify in mouse ECG. Therefore, the aortic diameter in systole should be measured at physiologic systole, defined by visual inspection (Figure 4I). The cardiac phase when the aorta is maximally expanded should be midsystole. End-diastole is easily defined at the R wave of the ECG (Figure 4I). Aortic measurements in end-diastole are simpler than those in midsystole in terms of distinguishing the cardiac cycle.</li>
		<li>Draw a line in the center of the aortic lumen. This center line will be used to ensure that the measurement lines are perpendicular to the aorta (Figure 4B,D and Supplemental Figure 1).</li>
		<li>Draw perpendicular lines through the center line from the luminal inner edge to inner edge at the aortic sinus and maximal ascending aortic levels (Figure 4B,D and Supplemental Figure 1).</li>
		<li>Measure the aortic diameter in at least three separate heartbeats and calculate the mean of the measurements. 
		NOTE: The Vevo2100 system uses the Vevo LAB analysis software for measurements of aortic dimension. Brief explanations for each button are as follows. Measurement mode (Supplemental Figure 1A): this mode must be selected for aortic measurements. The slider of a cine loop (Supplemental Figure 1B): the ultrasound frame is selected using this slider. Traced distance (Supplemental Figure 1C): the center line is drawn with this function. Linear distance (Supplemental Figure 1D): the aortic dimension is measured using this function.</li>
	</ol>
	</li>
	<li>Analysis of abdominal aortic images
	<ol>
		<li>Launch the analysis software and open the ultrasound data.</li>
		<li>Select an aortic image for analysis from the cine loop (Figure 4E,G). 
		NOTE: Similar to thoracic aortic measurements, the cardiac cycle may affect the abdominal aortic diameter and area. Measurements should be determined at a consistent phase of the cardiac cycle.</li>
		<li>Draw a line across the largest luminal diameter, from the inner edge to the inner edge of the vessel lumen (Figure 4F,H).</li>
		<li>Trace the inner edge of the aortic lumen for the luminal area (Figure 4F,H).</li>
		<li>Acquire aortic measurements at a minimum of three separate heartbeats and calculate the mean of the data.</li>
	</ol>
	</li>
</ol>

The authors have nothing to disclosure.

This protocol provides a technical guide for the image acquisition of the thoracic and abdominal aorta in mice, using a high-frequency ultrasound system. Ultrasound aortic imaging has potential confounders, such as probe position and cardiac cycle, that may compromise the accuracy of the aortic measurements, particularly in the proximal thoracic aorta. This protocol describes detailed instructions and strategies for image acquisition, measurement, and data analysis, in order to accurately measure aortic dimensions.
For imaging the proximal thoracic aorta, there are several approaches to probe placement. The right parasternal long axis view shown in Figure 2A was used for ultrasound imaging in this protocol. This view facilitates the acquisition of high-quality images from the aortic sinus to the aortic arch portion. It is not optimal for the descending aorta because of interference of the ultrasonic waves. This protocol is applicable to most mouse models of thoracic aortic aneurysms because they exhibit luminal dilation predominantly in the aortic root to the ascending aorta. This includes chronic angiotensin II infusion that causes aneurysm formation in the ascending aorta of mice18,19,20,21,22,23. Mouse models of Marfan syndrome (fibrillin 1C1041G/+ and fibrillin 1mgR/mgR mice) display both aortic root and ascending aortic dilation23,24,25. Loeys-Dietz syndrome mouse models (postnatal deletion of TGF-&#946; receptor 1 or 2 in smooth muscle cells) also develop aneurysm in the aortic root and ascending aorta18,26,27,28. Therefore, the right parasternal long axis view is appropriate for aortic imaging in these mouse models of thoracic aortic aneurysms. On the other hand, the right parasternal short axis view has the potential to capture aortic images diagonally because aneurysms are often complicated by aortic tortuosity, which may cause an overestimation of diameters. Unlike the thoracic aorta, the short axis view was used for the imaging of the abdominal aorta in this protocol. Since aortic curvature and tortuosity are modest in the abdominal aorta compared to the thoracic aorta, the acquisition of images in the short axis view ameliorates underestimations of the aortic diameter. It is important to note that different probe positions provide different viewing angles, and the aortic diameter may be different in each view angle. Therefore, reliable aortic diameter measurements are enhanced by applying the same probe position for all images within a study. Interestingly, three-dimensional (3D) ultrasound images of the heart and aorta have been reported recently29,30,31,32. In addition, current ultrasound systems can obtain 3D images over time as four-dimensional images33. Thus, these 3D imaging technologies have the potential to demonstrate the aortic structure more precisely, which may solve the problem of probe positioning.
Ultrasound images can be captured in either 2D brightness mode (B-mode) or one-dimensional motion mode (M-mode). Although some articles have used M-mode for the measurement of aortic diameter, B-mode is preferable15,34,35,36. M-mode has the capacity to image in two dimensions to increase temporal and spatial resolution. However, this mode relies on the assumption that the aorta is a concentric cylinder being imaged perpendicularly to the ultrasonic waves. This assumption may not hold true in an aneurysmal state and the curvature of the ascending aorta makes this difficult, even in nonaneurysmal states. In addition, the aorta does not remain in a fixed position throughout the cardiac cycle37. Therefore, M-mode may cause measurement errors, including over- and underestimations.
It is also important to note that the cardiac cycle affects the luminal diameter in the aorta. As expected, the aortic diameter in systole is greater than in diastole (Figure 4A-H), which is associated with aortic wall elasticity and strain. Aortic wall elasticity and strain can be calculated from the difference of aortic diameters between systole and diastole. Elasticity and strain are decreased in aneurysmal aortas compared to normal aortas31,34,35,38,39,40. Aortic stiffness cannot be measured directly by ultrasound. Measuring pulse wave velocity (PWV) can evaluate its stiffness as a proxy, which is reported to be increased in aneurysmal aortas31,35,41,42. PWV is calculated by the transit time between two arterial sites, using pulse wave Doppler images and their corresponding distance. For comparing aortic diameters, unlike clinical examination, there is no rigorous standardization in terms of cardiac phase for aortic measurements in mice. Therefore, it is still unclear which cardiac phase is appropriate for aortic measurements. However, to ensure reliable and reproducible comparisons, aortic diameters should be measured in a defined phase of the cardiac cycle.
This protocol provides detailed instructions for aortic imaging and data analysis in order to measure aortic dimensions accurately. The aortic measurement, using this protocol, was consistent with the actual ex vivo aortic diameter (Figure 5A). We also confirmed consistencies of inter- and intraobserver reproducibility (Figure 5B,C). All steps in this protocol, especially probe position and cardiac cycle, are necessary for accurate measurements. However, even when using appropriate procedures, artifacts during ultrasound imaging are unavoidable. The location of ribs and lung, as well as respiration and cardiac pulsation, can affect the image quality of the thoracic aorta. Intestinal gas can also cause artifacts in abdominal imaging. Thus, we suggest defining exclusion criteria when following this protocol in case of poor aortic images.
With the advent of high-resolution ultrasound systems, the aortic structure of mice can be examined in exquisite detail, both serially and conventionally, thereby greatly contributing to the understanding of aortic aneurysms. Ultrasound imaging, with the protocol as described above, is a reliable and reproducible noninvasive approach for quantifying aortic aneurysms in mice.

Aortic aneurysms are common vascular diseases characterized by a permanent luminal dilation of the thoracic and/or abdominal aorta1,2,3,4. No pharmacological therapies have been established to prevent the dilation and rupture of aortic aneurysms, which emphasizes the need for insights into pathogenic mechanisms. To elucidate the mechanisms of aortic aneurysms, mouse models produced by genetic or chemical manipulations have been widely used4,5,6,7,8,9,10,11,12. The accurate quantification of the aortic diameter in mice is the basis of aortic aneurysm research.
The development of high-frequency ultrasound has increased the spatial and temporal resolution of images to detect small differences in aortic dimensions13,14,15. This has enabled the sequential measurement of aortic diameters in mice, and thus, it has become the preferred method for measuring aortic diameters in murine studies of aortic aneurysms. Although ultrasound imaging is a simple technique, knowledge of aortic anatomy and physiology is required to acquire appropriate images for accurate measurements, data analysis, and interpretation. The aorta is a pulsating cylindrical organ with variable curvatures in the proximal thoracic region16. This contributes to the potential for an inaccurate determination of aortic dimensions in the commonly acquired two-dimensional (2D) images. The accuracy of aortic measurements could be compromised further by aortic tortuosity in aneurysmal state17. To obtain reliable and reproducible measurements of aortic dilations, this protocol provides a practical guide for the use of a high-resolution ultrasound system to measure proximal thoracic and abdominal aortic diameters in mice.

<table><tbody><tr><td>&lt;strong&gt;Name of Reagent&lt;/strong&gt;</td><td></td><td></td><td></td></tr><tr><td>Isothesia (Isoflurane)</td><td>Henry Schin</td><td>NDC11695-6776-2</td><td>Anesthetic Agent</td></tr><tr><td>Omnicon F/Air Anesthesia Gas Filter Canister</td><td>A.M. Bickford Inc.</td><td>80120</td><td>Scavenging System for Anesthesia</td></tr><tr><td>Puralube Vet Ointment</td><td>Dechra</td><td>NDC17033-211-38</td><td>Lubricating Eye Drops</td></tr><tr><td>Aquasonic&amp;nbsp;</td><td>Parker Laboratories</td><td>01-08</td><td>Ultrasound Gel</td></tr><tr><td>Nair</td><td>Nair</td><td></td><td>Depilliating Cream</td></tr><tr><td>Transeptic Transducer Cleaning Solution</td><td>Parker Laboratories</td><td>341-09-25</td><td>Cleaning spray for probes</td></tr><tr><td>&lt;strong&gt;Name of Equipment&lt;/strong&gt;</td><td></td><td></td><td></td></tr><tr><td>Vevo 2100</td><td>VisualSonics</td><td>Vevo 2100</td><td>Ultrasound Machine</td></tr><tr><td>Vevo LAB 3.0.0</td><td>VisualSonics</td><td>Vevo LAB 3.0.0</td><td>Ultrasound Analysis Software</td></tr><tr><td>MS-550D</td><td>VisualSonics</td><td>MS-550D</td><td>Ultrasound Probe</td></tr><tr><td>EX3 Vaporizer</td><td>Patterson Veterinary</td><td>EX 3</td><td>Analogue Anestheic Vaporizer</td></tr><tr><td>Heating Pad</td><td>Sunbeam</td><td>E12107</td><td>Heating Pad</td></tr></tbody></table>

ultrasound imaging of the thoracic and abdominal aorta in mice to determine aneurysm dimensions

Contemporary high-resolution ultrasound instruments have sufficient resolution to facilitate the measurement of mouse aortas. These instruments have been widely used to measure aortic dimensions in mouse models of aortic aneurysms. Aortic aneurysms are defined as permanent dilations of the aorta, which occur most frequently in the ascending and abdominal regions. Sequential measurements of aortic dimensions by ultrasound are the principal approach for assessing the development and progression of aortic aneurysms in vivo. Although many reported studies used ultrasound imaging to measure aortic diameters as a primary endpoint, there are confounding factors, such as probe position and cardiac cycle, that may impact the accuracy of data acquisition, analysis, and interpretation. The purpose of this protocol is to provide a practical guide on the use of ultrasound to measure the aortic diameter in a reliable and reproducible manner. This protocol introduces the preparation of mice and instruments, the acquisition of appropriate ultrasound images, and data analysis.

Representative ultrasound images of nonaneurysmal proximal thoracic and abdominal aorta are shown in Figure 3A and Figure 3C, respectively. The ascending aorta is located next to the pulmonary artery and forms a curved tube with three branches in the arch region: the innominate artery, the left common carotid artery, and the left subclavian artery (Figure 3A). The abdominal aorta is detected dorsally to the inferior vena cava (Figure 3D). Representative images of thoracic and abdominal aortic aneurysms with profound dilations, as compared with normal diameters in Figure 3A and Figure 3D, are shown in Figure 3B and Figure 3H, respectively. All ultrasound images were captured at end-diastole.
Representative thoracic and abdominal aortic ultrasound images were captured at the midsystole and end-diastole (Figure 4A,C,E,G). Representative images showing measurements are presented in Figure 4B,D,F,H. The green line in the center of the ascending aorta was used for standardizing the aortic sinus and ascending aortic diameter (Figure 4B,D). Lines were drawn perpendicularly to the green line between the two inner edges of the lumen at the aortic sinus (yellow line) and the maximal ascending aortic diameter (red line). The luminal diameters of the thoracic and abdominal aortas were different between systole and diastole (Figure 4A-H). For the abdominal aorta, the maximal aortic diameter (red) and luminal area (green) were measured (Figure 4F,H). A representative image of the monitor electrocardiogram is shown in Figure 4I. The cardiac cycle needs to be considered for accurate measurements. The end-diastole and systole are indicated by the white dotted and pink lines, respectively.
To validate the accuracy and reproducibility of this protocol, we performed a pilot study. Representative thoracic aortic ultrasound and ex vivo images are shown in Figure 5A. There was no major difference in diameters measured between these images for the ascending aortic diameter (ultrasound: 1.67 mm vs. ex vivo: 1.65 mm). Since the aortic sinus was difficult to see in the ex vivo image, the aortic sinus diameter was not measured ex vivo. The inter- and intraobserver reproducibility of this protocol are shown in Figure 5B,C. To determine potential variabilities, ultrasound imaging was performed by two observers independently, namely by an experienced cardiologist and a nonexperienced undergraduate student who is learning this technique, on two different days, using the same mice (n = 5). All dots were located between the mean ± 1.96 SD in Figure 5B,C, which indicates no major inter- or intraobserver variabilities for this protocol.
<img alt="Anesthesia setup with induction chamber, vaporizer, gel warmer; surgical preparation process." class="xfigimg" src="/files/ftp_upload/59013/59013fig1.jpg" data-altupdatedat="1747911167000" data-alt="Figure 1"> 
Figure 1: Workstation setup. The workstation includes the induction chamber for anesthesia, anesthetic scavenging filters, the heated platform, the ultrasound gel, and the gel warmer. <a href="https://www.jove.com/files/ftp_upload/59013/59013fig1large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Ultrasound probe positions for thoracic aorta imaging in rat; includes aortic ultrasound result." class="xfigimg" src="/files/ftp_upload/59013/59013fig2.jpg" data-altupdatedat="1747911167000" data-alt="Figure 2"> 
Figure 2: Examples of probe placement for proximal thoracic and abdominal aortic imaging. Probe placement for (A) the right and (B) the left parasternal long axis view of the aortic root, ascending and arch regions, and (C) the short axis view of the abdominal aorta. (D) A representative monitor image of the ultrasound system. The black arrows indicate the reference marker on the probe. The yellow arrow indicates the side of the reference marker. <a href="https://www.jove.com/files/ftp_upload/59013/59013fig2large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Ultrasound images of thoracic and abdominal aorta; aneurysmal and non-aneurysmal views, diagnostic analysis." class="xfigimg" src="/files/ftp_upload/59013/59013fig3.jpg" data-altupdatedat="1747911167000" data-alt="Figure 3"> 
Figure 3: Representative ultrasound images of the thoracic and abdominal aorta. (A) Nonaneurysmal and (B) aneurysmal ascending aorta, from the right parasternal long axis view. (C) Nonaneurysmal ascending aorta, from the left parasternal long axis view. (D) Nonaneurysmal and (E) aneurysmal abdominal aorta. Asc Ao = ascending aorta, IA = innominate artery, LCA = left common carotid artery, LSA = left subclavian artery, PA = pulmonary artery, Sinus = aortic sinus, IVC = inferior vena cava, and Abd Ao = abdominal aorta. The yellow triangles indicate an aortic aneurysm. <a href="https://www.jove.com/files/ftp_upload/59013/59013fig3large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Thoracic and abdominal aorta ultrasound; systole and diastole measurements; ECG graph integration." class="xfigimg" src="/files/ftp_upload/59013/59013fig4.jpg" data-altupdatedat="1747911167000" data-alt="Figure 4"> 
Figure 4: Measurements of aortic images. Images of the thoracic aorta captured at(A) the midsystole and (C) the end-diastole. Images showing measurements of aortic diameters in the proximal thoracic aortic region during (B) midsystole and (D) diastole. The green line indicates the center of the ascending aorta. The yellow and red lines indicate diameters of the aortic sinus and ascending aorta, respectively. Digits in yellow and red colors indicate actual diameters of the aortic sinus and ascending aorta, respectively. Images of abdominal aorta captured at (E) the midsystole and (G) the end-diastole. Images showing measurements of the suprarenal aorta during (F) midsystole and (H) end-diastole. The red and green lines indicate the diameter and luminal area of the abdominal aorta, respectively. Digits in red and green colors indicate the actual diameter and are of the abdominal aorta, respectively. (I) Monitor electrocardiogram (ECG) recorded during the image acquisitions. The green and yellow lines indicate the ECG and respiratory cycle, respectively. The white dotted line indicates the end-diastole, and the purple line indicates systole. P = P wave and R = R wave. <a href="https://www.jove.com/files/ftp_upload/59013/59013fig4large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Ultrasound and ex vivo aortic measurements with inter-observer and intra-observer variability graphs." class="xfigimg" src="/files/ftp_upload/59013/59013fig5.jpg" data-altupdatedat="1747911167000" data-alt="Figure 5"> 
Figure 5: Accuracy and reproducibility of ultrasound imaging. (A) Representative images of thoracic aortic ultrasound and ex vivo images in C57BL/6J male mice (10-12 weeks old). Bland-Altman plots show (B) inter- and (C) intraobserver variabilities of this protocol. Asc Ao = ascending aorta, IA = innominate artery, LCA = left common carotid artery, LSA = left subclavian artery, PA = pulmonary artery, and Sinus = aortic sinus. The green line indicates the center of the ascending aorta. The yellow and red lines indicate the diameters of the aortic sinus and ascending aorta, respectively. Digits in red colors denote the actual diameters of the ascending aorta measured in ultrasound and ex vivo images. The black dotted lines indicate the mean and mean ± 1.96 SD. <a href="https://www.jove.com/files/ftp_upload/59013/59013fig5large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Ultrasound echocardiography analysis diagram; ascending aorta measurement, sinus data, cardiac tool." src="/files/ftp_upload/59013/59013supfig1.jpg" data-altupdatedat="1747911167000" data-alt="Supplemental Figure 1"> 
Supplemental Figure 1: Example image of ultrasound analysis software. Ultrasound data analysis must be performed in (A) measurement mode. One aortic ultrasound image is selected for analysis from the cine loop using (B) the slider of a cine loop. The center line is drawn using (C) the traced distance function. The aortic dimension is measured by (D) the linear distance function. <a href="https://www.jove.com/files/ftp_upload/59013/59013supfig1large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>

Watch this Scientific Journal Video about Ultrasound Imaging of the Thoracic and Abdominal Aorta in Mice to Determine Aneurysm Dimensions at JoVE.com

Ultrasound Imaging of the Thoracic and Abdominal Aorta in Mice to Determine Aneurysm Dimensions

Ultrasound imaging has become a common modality to determine the luminal dimensions of thoracic and abdominal aortic aneurysms in mice. This protocol describes the procedure to acquire reliable and reproducible two-dimensional ultrasound images of the ascending and abdominal aorta in mice.

Contemporary high-resolution ultrasound instruments have sufficient resolution to facilitate the measurement of mouse aortas. These instruments have been ...

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19.2K Views.  University of Kentucky. Hoge resolutie echografie metingen zijn op grote schaal gebruikt om aorta afmetingen bij muizen te meten. Dit protocol biedt een praktische leidraad voor de toepassing van deze modaliteit. Dit protocol vergemakkelijkt de verwerving van reproduceerbare en nauwkeurige tweedimensionale metingen van de diameter van de thoracale en abdominale aorta's bij muizen.Het aantonen van de procedure zal Dr.Hasashi Sawada, een postdoctoraal geleerde, en Jessica Morrleghen een onderzoeker, zowel aan d...

Video: Ultrasound Imaging of the Thoracic and Abdominal Aorta in Mice to Determine Aneurysm Dimensions

Murine Echocardiography and Ultrasound Imaging

Rodent models of cardiac pathophysiology represent a valuable research tool to investigate mechanism of disease as well as test new therapeutics.1 Echocardiography provides a powerful, non-invasive tool to serially assess cardiac morphometry and function in a living animal.2 However, using this technique on mice poses unique challenges owing to the small size and rapid heart rate of these animals.3 Until recently, few ultrasound systems were capable of performing quality echocardiography on mice, and those generally lacked the image resolution and frame rate necessary to obtain truly quantitative measurements. Newly released systems such as the VisualSonics Vevo2100 provide new tools for researchers to carefully and non-invasively investigate cardiac function in mice. This system generates high resolution images and provides analysis capabilities similar to those used with human patients. Although color Doppler has been available for over 30 years in humans, this valuable technology has only recently been possible in rodent ultrasound.4,5 Color Doppler has broad applications for echocardiography, including the ability to quickly assess flow directionality in vessels and through valves, and to rapidly identify valve regurgitation. Strain analysis is a critical advance that is utilized to quantitatively measure regional myocardial function.6 This technique has the potential to detect changes in pathology, or resolution of pathology, earlier than conventional techniques. Coupled with the addition of three-dimensional image reconstruction, volumetric assessment of whole-organs is possible, including visualization and assessment of cardiac and vascular structures. Murine-compatible contrast imaging can also allow for volumetric measurements and tissue perfusion assessment.

This video demonstrates use of a rail-mounted high-frequency ultrasound probe to perform echocardiography on an anesthetized mouse. The methods describe both conventional two-dimensional and M-mode measurements of cardiac function in addition to newer, more powerful tools such as color Doppler, strain analysis, as well as general and targeted contrast imaging.

Rodent models of cardiac pathophysiology represent a valuable research tool to investigate mechanism of disease as well as test new therapeutics.1  ...

Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging

Transverse aortic constriction (TAC) in mice has been used as a valuable model to study mechanisms of cardiac hypertrophy and heart failure1. A reliable noninvasive method is essential to assess real-time cardiac morphological and functional changes in animal models of heart disease. Transthoracic echocardiography represents an important tool for noninvasive assessment of cardiac structure and function2. Here we used a high-resolution ultrasound imaging system to monitor myocardial remodeling and heart failure progression over time in a mouse model of TAC. B-mode, M-mode, and Doppler imaging were used to precisely assess cardiac hypertrophy, ventricular dilatation, and functional deterioration in mice following TAC. Color and pulse wave (PW) Doppler imaging was used to noninvasively measure pressure gradient across the aortic constriction created by TAC and to assess transmitral blood flow in mice. Thus transthoracic echocardiographic imaging provides comprehensive noninvasive measurements of cardiac dimensions and function in mouse models of heart disease.

The goal of this protocol is to noninvasively assess cardiac structural and functional changes in a mouse model of heart disease created by transverse aortic constriction, using B- and M-mode echocardiography and color/pulse wave Doppler imaging.

Transverse aortic constriction (TAC) in mice has been used as a valuable model to study mechanisms of cardiac hypertrophy and heart failure1. A reliable ...

Murine Echocardiography of Left Atrium, Aorta, and Pulmonary Artery

The present methodology teaches the investigator how to measure and use the LAV as a surrogate of chronic elevations in Left Ventricular diastolic pressure through echocardiography, as well as to obtain measurements of the Aorta and PA diameter in mice.
Mice older than 10 d of age can be analyzed using the present technique. The technique is composed of 3 main steps: set-up, image acquisition, and image analysis. The set-up step consists of getting the mouse anesthetized with 1% isoflurane, shaving it, and taping it in a supine position to a heated EKG board where the image acquisition will take place. The image acquisition step consists of learning to identify the cardiac structures and obtaining all the required images with its correspondent probe and axis in order to be able to calculate volumes and diameters. The image analysis step consists of measuring the previously acquired images with the aid of computer software.
Advantages of the proposed technique include a fast (15 min) procedure that would allow the researcher to evaluate interventions in a non-invasive, non-terminal approach and therefore follow the same mouse over time; each mouse can be used as its own control. This fact plus having the same operator perform all the acquisition and analysis for the entire experiment minimizes the limitation of operator-dependency. The present methodology is useful for mouse researchers in cardiovascular and pulmonary medicine.

The following protocol describes the methodology for the acquisition and analysis of echocardiographic images used to obtain the Left Atrial Volume (LAV), Aorta (Ao) diameter, and Pulmonary Artery (PA) diameter in mice. This technique is a non-invasive, non-terminal procedure that allows assessment of the cardiopulmonary function.

The present methodology teaches the investigator how to measure and use the LAV as a surrogate of chronic elevations in Left Ventricular diastolic ...

Measurement of Pulse Propagation Velocity, Distensibility and Strain in an Abdominal Aortic Aneurysm Mouse Model

An abdominal aortic aneurysm (AAA) is defined as a localized dilation of the abdominal aorta that exceeds the maximal intraluminal diameter (MILD) by 1.5 times of its original size. Clinical and experimental studies have shown that small aneurysms may rupture, while a subpopulation of large aneurysms may remain stable. Thus, in addition to the measurement of intraluminal diameter of the aorta, knowledge of structural traits of the vessel wall may provide important information to assess the stability of the AAA. Aortic stiffening has recently emerged as a reliable tool to determine early changes in the vascular wall. Pulse propagation velocity (PPV) along with the distensibility and radial strain are highly useful ultrasound-based methods relevant for assessing aortic stiffness. The primary purpose of this protocol is to provide a comprehensive technique for the use of ultrasound imaging system to acquire images and analyze the structural and functional properties of the aorta as determined by MILD, PPV, distensibility and radial strain.

This manuscript describes a detailed protocol for using high frequency ultrasound imaging to measure luminal diameter, pulse propagation velocity, distensibility and radial strain on a mouse model of abdominal aortic aneurysm.

An abdominal aortic aneurysm (AAA) is defined as a localized dilation of the abdominal aorta that exceeds the maximal intraluminal diameter (MILD) by 1.5 ...

Immunology and Infection

Quantitative Micro-CT Analysis of Aortopathy in a Mouse Model of &#946;-aminopropionitrile-induced Aortic Aneurysm and Dissection

Aortic aneurysm and dissection is associated with significant morbidity and mortality in the population and can be highly lethal. While animal models of aortic disease exist, in vivo imaging of the vasculature has been limited. In recent years, micro-computerized tomography (micro-CT) has emerged as a preferred modality for imaging both large and small vessels both in vivo and ex vivo. In conjunction with a method of vascular casting, we have successfully used micro-CT to characterize the frequency and distribution of aortic pathology in &#946;-aminopropionitrile-treated C57/Bl6 mice. Technical limitations of this method include variations in the quality of the perfusion introduced by poor animal preparation, the application of proper methodologies for vessel size quantification, and the non-survivability of this procedure. This article details a methodology for the intravascular perfusion of a lead-based radiopaque silicone rubber for the quantitative characterization of aortopathy in a mouse model of aneurysm and dissection. In addition to visualizing aortic pathology, this method may be used for examining other vascular beds in vivo or vascular beds removed post-mortem.

This article describes a detailed methodology of using a radiopaque lead-based silicone rubber to perfuse the murine vasculature for aortic diameter quantification in a mouse model of aortic aneurysm and dissection.

Aortic aneurysm and dissection is associated with significant morbidity and mortality in the population and can be highly lethal. While animal models of ...

Drug Treatment by Central Venous Catheter in a Mouse Model of Angiotensin II Induced Abdominal Aortic Aneurysm and Monitoring by 3D Ultrasound

Since pharmaceutical treatment options are lacking in the clinical management of abdominal aortic aneurysm (AAA), animal models, in particular mouse models, are applied to advance the understanding of the disease pathogenesis and to identify potential therapeutic targets. Testing novel drug candidates to block AAA growth in these models generally requires repeated drug administration during the time course of the experiment. Here, we describe a compiled protocol for AAA induction, insertion of an intravenous catheter to facilitate prolonged therapy, and serial AAA monitoring by 3D ultrasound. Aneurysms are induced in apolipoprotein E (ApoE) deficient mice by angiotensin II release over 28 days from osmotic mini-pumps implanted subcutaneously into the mouse back. Subsequently, the surgical procedure for external jugular vein catheterization is conducted to allow for daily intravenous drug treatment or repeated blood sampling via a subcutaneous vascular access button. Despite the two dorsal implants, the monitoring of AAA development is readily facilitated by sequential semi-automated 3D ultrasound analysis, which yields comprehensive information on the expansion of aortic diameter and volume and on aneurysm morphology, as illustrated by experimental examples.

This protocol describes the consecutive implantation of an osmotic pump to induce abdominal aortic aneurysm by angiotensin II release in apolipoprotein E (ApoE) deficient mice and of a vascular access port with a jugular vein catheter for repeated drug treatment. Monitoring of aneurysm development by 3D ultrasound is effectively conducted despite dorsal implants.

Since pharmaceutical treatment options are lacking in the clinical management of abdominal aortic aneurysm (AAA), animal models, in particular mouse ...

Investigating the Molecular Mechanisms Underlying Murine Abdominal Aortic Aneurysm

A Mouse Abdominal Aortic Aneurysm Model by Periadventitial Calcium Chloride and Elastase Infiltration

Abdominal aortic aneurysm (AAA) is a life-threatening disease associated with high mortality rates. It is characterized by the permanent dilation of the abdominal aorta with at least a 50% increase in arterial diameter. Various animal models of AAA have been introduced to mimic the pathophysiological changes and study the underlying mechanisms of AAA. Among these models, the calcium chloride (CaCl2)- and elastase-induced AAA models are commonly used in mice. However, these methods have certain limitations. Traditional intraluminal porcine pancreatic elastase (PPE) perfusion is associated with high technical difficulty and a high rupture rate, while periadventitial administration of PPE yields inconsistent results. In addition, the CaCl2-induced AAA model lacks human AAA features, such as atherothrombosis and aneurysm rupture. Therefore, the combined application of CaCl2 and PPE has been proposed as an approach to enhance success rates and induce greater diameter increases in AAA animal models. This manuscript presents a comprehensive protocol for establishing a mouse AAA model through periaortic infiltration of PPE and CaCl2 in the infrarenal segment of the abdominal aorta. By following this protocol, we can achieve an AAA formation rate of approximately 90% with technical simplicity and reproducibility. Further ultrasound and histological experiments confirm that this model effectively replicates the morphological and pathological changes observed in human AAA.

Author Spotlight: Development and Characterization of a Mouse Model for Abdominal Aortic Aneurysm

This manuscript presents a protocol for establishing a mouse abdominal aortic aneurysm model using calcium chloride and elastase, combining the advantages of previous modeling methods. This model can be utilized to investigate the pathophysiological mechanisms underlying abdominal aortic aneurysms.

Abdominal aortic aneurysm (AAA) is a life-threatening disease associated with high mortality rates. It is characterized by the permanent dilation of the ...

Mouse Abdominal Aortic Aneurysm Model Induced by Perivascular Application of Elastase

Abdominal aortic aneurysm (AAA), although primarily asymptomatic, is potentially life-threatening as the rupture of AAA usually has a devastating outcome. Currently, there are several distinct experimental models of AAA, each emphasizing a different aspect in the pathogenesis of AAA. The elastase-induced AAA model is the second most used rodent AAA model. This model involves direct infusion or application of porcine pancreatic elastase (PPE) to the infrarenal segment of the aorta. Due to technical challenges, most elastase-induced AAA model nowadays is performed with the external application rather than an intraluminal infusion of PPE. The infiltration of elastase will cause degradation of elastic lamellae in the medial layers, resulting in the loss of aortic wall integrity and subsequent dilation of the abdominal aorta. However, one disadvantage of the elastase-induced AAA model is the inevitable variation of how the surgery is performed. Specifically, the surgical technique of isolating the infrarenal segment of the aorta, the material used for aorta wrapping and PPE incubation, the enzymatic activity of PPE, and the time duration of PPE application can all be important determinants that affect the eventual AAA formation rate and aneurysm diameter. Notably, the difference in these factors from different studies on AAA can lead to reproducibility issues. This article describes a detailed surgical process of the elastase-induced AAA model through direct application of PPE to the adventitia of the infrarenal abdominal aorta in the mouse. Following this procedure, a stable AAA formation rate of around 80% in male and female mice is achievable. The consistency and reproducibility of AAA studies using an elastase-induced AAA model can be significantly enhanced by establishing a standard surgical procedure.

The present protocol describes a standardized surgical method for the elastase-induced AAA model through the direct application of elastase to the adventitia of infrarenal abdominal aorta in mice.

Abdominal aortic aneurysm (AAA), although primarily asymptomatic, is potentially life-threatening as the rupture of AAA usually has a devastating outcome. ...

Biology

Abdominal Aortic Aneurysm Development in Mice Using the Elastase and BAPN Method

Advanced Abdominal Aortic Aneurysm Modeling in Mice by Combination of Topical Elastase and Oral &#223;-aminopropionitrile

The topical elastase murine model of abdominal aortic aneurysm (AAA) is enhanced when combined with &#223;-aminopropionitrile (BAPN)-supplemented drinking water to reliably produce true infrarenal aneurysms with behaviors that mimic human AAAs. Topically applying elastase to the adventitia of the infrarenal aorta causes structural damage to the elastic layers of the aortic wall and initiates aneurysmal dilation. Co-administering BAPN, a lysyl oxidase inhibitor, promotes sustained wall degeneration by reducing collagen and elastin crosslinking. This combination results in large AAAs that progressively expand, form intraluminal thrombus, and are capable of rupture. Refining surgical techniques, such as circumferentially isolating the entire infrarenal aortic segment, can help standardize the procedure for a consistent and thorough application of porcine pancreatic elastase despite different operators and anatomic variations between mice. Therefore, the elastase/BAPN model is a refined approach to surgically inducing AAA in mice, which may better recapitulate human aneurysms and provide additional opportunities to study aneurysm growth and rupture risk.

Author Spotlight: Enhanced Murine AAA Model Using Elastase to Mimic Human Aneurysms

This protocol describes a methodical surgical approach to modeling advanced abdominal aortic aneurysms in mice by a combination of directly applying elastase to the infrarenal aorta and administering &#223;-aminopropionitrile through drinking water.

The topical elastase murine model of abdominal aortic aneurysm (AAA) is enhanced when combined with &#223;-aminopropionitrile (BAPN)-supplemented drinking ...

High-frequency Ultrasound Imaging of the Abdominal Aorta

Source: Amelia R. Adelsperger, Evan H. Phillips, and <a href="https://www.jove.com/author/Craig+J._Goergen">Craig J. Goergen</a>, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana
High-frequency ultrasound systems are used to acquire high resolution images. Here, the use of a state-of-the-art system will be demonstrated to image the morphology and hemodynamics of small pulsatile arteries and veins found in mice and rats. Ultrasound is a relatively inexpensive, portable, and versatile method for the noninvasive assessment of vessels in humans as well as large and small animals. These are several key advantages that ultraound offers compared to other techniques, such as computed tomography (CT), magnetic resonance imaging (MRI), and near-infrared fluorescence tomography (NIRF). CT requires ionizing radiation and MRI can be prohibitively expensive and even impractical in some scenarios. NIRF, on the other hand, is limited by the penetration depth of light required to excite the fluorescent contrast agents.
Ultrasound has limitations in terms of imaging depth; however, this may be overcome by sacrificing resolution and using a lower frequency transducer. Abdominal gas and excess body weight can severely diminish image quality. In the first case, the propagation of sound waves is limited, while in the latter case, they are attenuated by overlying tissues, such as fat and connective tissue. As a result, no contrast or faint contrast may be observed. Finally, ultrasound is a highly user-dependent technique, requiring the sonographer to be familiar with anatomy and to be able to work around issues, such as the appearance of imaging artifacts or acoustic interference.

High-frequency Ultrasound Imaging and Image Acquisition

Source: Amelia R. Adelsperger, Evan H. Phillips, and Craig J. Goergen, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, 
...

Ultrasound Imaging of the Thoracic and Abdominal Aorta in Mice to Determine Aneurysm Dimensions

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