The publication was prepared under a project financed from funds granted by the Ministry of Science and Higher Education in the &#34;Regional Initiative of Excellence&#34; program for the years 2019-2022, project number 016/RID/2018/19, the amount of funding 11 998 121.30 PLN, and by subvention SUB.A020.21.018 of the Medical University in Wroclaw, Poland.

This article presents a protocol that adheres to the guidelines of the Bioethics Committee at the Wroclaw Medical University in Poland. All participants were legally competent and provided written informed consent to participate in the study. They were also informed that they had the option to withdraw at any time without giving a reason.
1. Cleaning the AEC
NOTE: The cleaning can be done earlier than on the day of the experiment.
<ol>
	<li>Vacuum all the surfaces, including the furniture and the floor, with a high-efficiency particulate air (HEPA) filter vacuum cleaner.</li>
	<li>Clean all washable surfaces with a moisture wipe, including the furniture, the walls, the windows, and the floor.</li>
	<li>Turn on the compressor, which circulates air through the AEC system (allergen supply duct).</li>
	<li>Turn on the floor and ceiling fans so that the incoming air is regularly mixed under turbulent conditions.</li>
	<li>Blow the allergen supply duct with clean air for 30 min by setting the &#34;injection length&#34; and &#34;break between injections &#34; of the feeder control station to their maximum values.</li>
	<li>Check the contamination by the allergen by monitoring the particle number on the laser particle counter (LPC)21.
	<ol>
		<li>In the main menu, press Configuration | Sample. Use the following parameters: sample for 1 min, 000 cycles, 0 min delay, hold for 0 min, and units of cubic meters (m3). 
		NOTE: The LPC will start counting the particles immediately and then count the particles for 1 min without an interval between each measurement. The LPC will measure the samples until manually stopped and then calculate the particles per cubic meter (p/m3).</li>
		<li>In the main menu, press Configuration | Particles. Select all options. 
		NOTE: The LPC will measure all the particles up to 100 &#181;m (full range).</li>
		<li>Read out the result in the computer program (e.g., LMS Express 7). 
		&#8203;NOTE: The cabin is clean when the number of particles per cubic meter (p/m3) is less than 50 p/m3 and the particles are in the range between 0-100 &#181;m for at least 10 min.</li>
	</ol>
	</li>
</ol>
2. Operating the AEC
NOTE: The atmosphere in the cabin must be regularly monitored by an engineer, who establishes that the parameters are constant during the trial. The parameters should be stabilized before the participants enter.
<ol>
	<li>Environment</li>
	<li>Turn on the compressor, which circulates air throughout the AEC.
	<ol>
		<li>Adjust the temperature to 21 &#176;C &#177; 0.5 &#176;C on the temperature control system (Table of Materials). 
		NOTE: The temperature may vary between 18&#176;C and 27 &#176;C, if necessary.</li>
		<li>Turn on the floor and ceiling swirl fans.</li>
		<li>Turn on the humidifier on the feeder control station (Table of Materials).</li>
		<li>Set the air change per hour (ACH) to be between 5 and 20 by setting the knob &#34;air supply&#34; on the feeder control station to the position between 40%-100%. Measure the relative humidity and CO2 concentration with an air quality meter. 
		NOTE: Fresh external air is drawn in via HEPA filters. Control the relative humidity (typically 40% to 58%) and carbon dioxide (CO2) concentration (below 900 parts per million [ppm]). Adjust the ACH so that both the humidity and CO2 are within the normal range. Humidity and CO2 values are very susceptible to the number of participants.</li>
	</ol>
	</li>
	<li>Generation and counting of particles 
	NOTE: Standardized and lyophilized allergen extracts are used. The particles are injected into the air supply duct and blown into the AEC through a computer-controlled feeder. The particle concentration can be adjusted between 500/m3 and 10,000/m3. A homogeneous, spatially stable distribution of particles is obtained by turbulent mixing to ensure the allergen particles are circulating instead of falling and accumulating on the floor.
	<ol>
		<li>Set the LPC to count the particles for 1 min (repeat step 1.6.1).</li>
		<li>Set the value of the monitored particles in the range of 0-20 &#181;m. In the main menu, press Configuration | Particles. Tick &#34;5, 10, 20 &#181;m&#34;. The LPC will measure all the particles in the range of 0-20 &#181;m. 
		NOTE: The particles can be classified into ranges, including 0-20 &#181;m, 20-50 &#181;m, and 50-100 &#181;m, if needed for monitoring a different allergen.</li>
		<li>Put the allergen in the feeder. Set the &#34;injection length&#34; to 100 ms (range 10-200 ms) and the &#34;break between injections&#34; to 1.5 min (range 0.3-3.0 min) on the feeder control station. 
		NOTE: For the ALL-MED AEC validation, dried, purified Dermatophagoides pteronyssinus (Dp) mite bodies (Table of Materials) were used for the HMD challenge, and 5,000 p/m3 was the optimal concentration20.</li>
		<li>Monitor the number of particles (p/m3). Adjust both parameters on an ongoing basis by changing their values.</li>
	</ol>
	</li>
	<li>After each trial is completed, download all the measured data (p/m3, CO2 concentration) from the computer to an external drive. Analyze the data (Figure 2).</li>
</ol>
3. Security measures
<ol>
	<li>Test the participants with a PCR test for severe acute respiratory syndrome coronavirus 2 virus (SARS-CoV-2) 36-24 h before they enter the AEC. Only permit participants with a negative PCR result to enter the AEC. 
	&#8203;NOTE: This step is not mandatory and depends on local coronavirus disease 2019 (COVID-19) restrictions. Patients in the cabin do not wear protective masks.</li>
</ol>
4. Examination in the cabin and clinical endpoints
NOTE: For the inclusion and exclusion criteria, as well as the participant characteristics, see Supplementary Table 1. The participants were exposed to HDM allergens at a concentration of 5,000 p/m3 for a duration of 120 min, according to the validation of the ALL-MED AEC20.
<ol>
	<li>Disinfect the participants&#39; hands before the examinations, because the device components they touch may be a source of infection transmission. This recommendation is essential, especially during a viral disease epidemic or pandemic.</li>
	<li>Constantly monitor the condition of the participants through the viewing window and be in voice contact via the microphone system (Table of Materials).</li>
	<li>Prior to the participant entering the AEC, request that they put on disposable coveralls with a hood (Table of Materials) to guard against the infiltration of non-allergen particles and the potential contamination of clothing.</li>
	<li>Before the participant enters the AEC, provide them with a box containing all the necessary instrument disposable tips for use during the examination: a spirometry tip and nose plug, a disposable inspiratory flow matter mask, a peak flow meter (PFM) tip, ARM tips, a remote controller for the questionnaire, one pack of handkerchiefs, and a biohazard plastic bag for nasal secretion.</li>
	<li>Perform clinical endpoints following the steps below. Repeat the ARM, PNIF, PERF, and FEV1 tests before the experiment and after 60 min and 120 min. Ensure that the participants complete the TNSS survey every 30 min (Figure 3). For participant comfort, perform the tests individually in a room next to the AEC. 
	NOTE: For efficient testing, have the participants enter the cabin at 10 min intervals. As a result, the measurements for each subject will be taken at different real times, with each patient spending a total of 120 min inside the AEC. The time shift also allows staff members to assist and interact with the participants during the testing process. In total, the AEC operates for approximately 210 min.
	<ol>
		<li>Nasal secretion (objective parameter) 
		NOTE: The participants should have identical packages of handkerchiefs and plastic bags. This is necessary in order to compare the weights.
		<ol>
			<li>Instruct the participants to place any used handkerchiefs in a plastic bag. After the 2 h challenge has been completed, have the participants also place any unused handkerchiefs in the same bag. If necessary, provide additional tissues and plastic bags.</li>
			<li>Collect all the bags after the trial is over. Determine the weight of nasal secretions by weighing the used handkerchiefs in the plastic bags. Subtract the weight of the unused handkerchiefs and plastic bags from each measurement to obtain the weight of the nasal secretions (Figure 4A).</li>
		</ol>
		</li>
		<li>Nasal symptoms survey (subjective assessment)
		<ol>
			<li>Display the survey questions on the TV screen.</li>
			<li>Ask the patient to self-assess before the challenge and every 30 min during the challenge by selecting the number on the remote that corresponds to the severity of each symptom (question). Assess nasal symptoms based on the total nasal symptom score (TNSS) survey (Table 1).</li>
			<li>Send the participant an email with the TNSS questionnaire. Ask them to complete the questionnaire at home at 4 h and 24 h after the challenge and send back the results.</li>
			<li>After the challenge is over, download the answers, and calculate the total score for each survey (Figure 4B).</li>
		</ol>
		</li>
		<li>Acoustic rhinometry (ARM) (objective parameter) 
		NOTE: In order to calculate the differences in minimal cross-sectional area (MCA), all the measurements of one participant must be saved in a single file. Otherwise, analysis will not be possible.
		<ol>
			<li>Perform the test three times: before the challenge, 60 min after the challenge, and 120 min after the challenge.</li>
			<li>Place the appropriate tip of the rhinometer head against the nostril (blue for the left nostril). Check if it is tight. Ask the participant to hold their breath for 3 s, and then start the program. 
			NOTE: In case of an unclear result, repeat the test.</li>
			<li>Repeat for the other nostril with the appropriate tip (red for the right nostril).</li>
			<li>After the challenge is over, calculate the MCA (Figure 4C).</li>
		</ol>
		</li>
		<li>Peak nasal inspiratory flow (PNIF) (objective parameter) 
		NOTE: PNIF directly measures the nasal airflow during maximal inspiration and determines the degree of nose obstruction.
		<ol>
			<li>Perform the test three times: before the challenge, 60 min after the challenge, and 120 min after the challenge.</li>
			<li>Ask the participant to deflate their lungs deeply. Then, place the disposable inspiratory flow metermask connected with the flow meter onto their face, and instruct them to breathe in through their nose to the maximum (Figure 4D).</li>
			<li>Make sure that the inspiratory flow meter is in a horizontal position throughout the whole test. Record the average of the best of three measurements.</li>
		</ol>
		</li>
		<li>Peak expiratory flow rate PEFR (safety parameter) 
		NOTE: PEFR is a reliable indicator of ventilation adequacy as well as airflow obstruction.
		<ol>
			<li>Perform the test three times: before the challenge, 60 min after the challenge, and 120 min after the challenge.</li>
			<li>Ask the participant to take in as deep a breath as possible, put their lips around the peak flow matter disposable tip, and exhale quickly and forcefully (Figure 4E).</li>
			<li>Record the average of the best of three measurements.</li>
			<li>After the challenge is over, provide the participant with a PFM. Ask them to perform the test at home at 4 h and 24 h after the challenge and send back the results.</li>
		</ol>
		</li>
		<li>Spirometry (safety lung parameter) 
		NOTE: Spirometry is performed according to the European Respiratory Society (ERS)22 standards to assess the safety and monitor possible bronchial obstruction23.
		<ol>
			<li>Perform the test three times: before the challenge, 60 min after the challenge, and 120 min after the challenge.</li>
			<li>Before the measurement, set the parameters on the spirometer for each participant: gender, age, weight, and height.</li>
			<li>Ask the participant to sit down and put on the nose plug. Then, have the participant place their lips around the spirometer disposable tip and breathe calmly and carefully.</li>
			<li>Ask the participant to take a deep breath and a strong exhalation without unnecessary delay, which can only be interrupted when the spirometer gives a signal. Repeat 3x.</li>
			<li>After examining all the participants, download the results, and record the forced expiratory volume in the first second (FEV1) (Figure 4F).</li>
		</ol>
		</li>
	</ol>
	</li>
	<li>If the participant&#39;s well-being or safety parameters deteriorate drastically during the allergen challenge, stop the test immediately.</li>
	<li>Keep the participants safe and comfortable after leaving the AEC facility by providing them with rescue medications (if needed).</li>
	<li>Conduct safety follow-up calls with each participant 24 h after the challenge.</li>
</ol>

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H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differential+induction+of+allergen-specific+IgA+responses+following+timothy+grass+subcutaneous+and+sublingual+immunotherapy.">Differential induction of allergen-specific IgA responses following timothy grass subcutaneous and sublingual immunotherapy.</a> Journal of Allergy and Clinical Immunology. 148 (4), 1061-1071 (2021).</li><li>Thwaites, R. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Absorption+of+nasal+and+bronchial+fluids:+Precision+sampling+of+the+human+respiratory+mucosa+and+laboratory+processing+of+samples.">Absorption of nasal and bronchial fluids: Precision sampling of the human respiratory mucosa and laboratory processing of samples.</a> Journal of Visualized Experiments. (131), e56413 (2018).</li><li>Zieglmayer, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Clinical+validation+of+a+house+dust+mite+environmental+challenge+chamber+model.">Clinical validation of a house dust mite environmental challenge chamber model.</a> Journal of Allergy and Clinical Immunology. 140 (1), 266-268 (2017).</li><li>Lueer, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=efficacy+and+repeatability+of+a+novel+house+dust+mite+allergen+challenge+technique+in+the+Fraunhofer+allergen+challenge+chamber.">efficacy and repeatability of a novel house dust mite allergen challenge technique in the Fraunhofer allergen challenge chamber.</a> Allergy. 71 (12), 1693-1700 (2016).</li><li>Pfaar, O., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Allergen+exposure+chambers:+Harmonizing+current+concepts+and+projecting+the+needs+for+the+future+-+An+EAACI+Position+Paper.">Allergen exposure chambers: Harmonizing current concepts and projecting the needs for the future - An EAACI Position Paper.</a> Allergy. 72 (7), 1035-1042 (2017).</li></ol>

Marek Jutel reports personal&#160;fees from ALK-Abello,Allergopharma, Stallergenes, Anergis,Allergy Therapeutics, Leti, HAL, GSK,Novartis, Teva, Takeda, and Chiesi. The other authors have nothing to disclose.

There are a limited number of AEC facilities operating globally. A variety of allergens have been tested in these facilities, with the most common being ragweed pollen, birch pollen, grass pollen, Japanese cedar pollen, and HDM. AECs are not classified as medicinal products (according to the Directive 2001/83/EC) or medical devices (according to the Medical Device Directive 93/42/EEC)24. AECs are considered a possible tool for the measurement of primary endpoints in dose-finding studies according to the European Medicines Agency (EMA) guidelines for the development of AIT products25,26.
Critical steps in the protocol 
It is essential to provide stable and sufficiently high allergen concentrations throughout the whole trial in the AEC. Research shows that AR patients do not develop allergic symptoms at low allergen concentrations20. Even moderate allergen concentrations do not trigger relevant symptoms27. Very high concentrations might cause severe reactions, such as bronchoconstriction. Therefore, optimal and sustainable allergen concentrations are key for a successful trial. Since AECs vary (as described in the introduction), each allergen used should be validated. The ALL-MED AEC is validated for the HDM allergen. It was found that the optimal endpoint for symptom assessment was 120 min, as symptoms reached a plateau after 60-90 min. The optimal challenge time and allergen concentration were selected based on challenges with different HMD concentrations at different times20. Notably, acute symptoms may occur after an allergen challenge, particularly an exacerbation of asthma.
According to the protocol, the participants complete TNSS surveys at five time points during the trial. It is essential that they do not see their previous responses in order to avoid self-suggestion. Therefore, if the questionnaires are completed on paper, the completed questionnaires should be collected immediately.
Modifications and troubleshooting of the method 
Different clinical endpoints can be used depending on the symptom to be observed during the challenge (e.g., the total ocular symptom score [TOSS] to assess rhinoconjunctivitis or the non-nasal symptom score [NNSS] for respiratory system assessment).
Rhinomanometry might be used as an alternative to acoustic rhinometry. Both methods are used to test nasal patency objectively. Rhinomanometry is a standard test for the nasal cavity. It enables an objective assessment of the patency of the nasal passages by measuring the resistance in the nasal cavity during inhalation and exhalation. Acoustic rhinometry is the study of the volume of the nasal cavities. The nasal cavity&#39;s patency is assessed by an ultrasound wave. There is no data available on which method is more accurate for AEC challenges28,29.
A nasal fluid collection from a single foam sponge and specific level measurements of IgA1, IgA2, IgG, IgG, IgG4, and IgE represent additional tests that can be done during the AEC challenge30,31. Serum and peripheral blood mononuclear cells (PBMCs) can also be collected to further determine the AIT molecular mechanisms.
Patients are not allowed to use medications that may influence the onset of allergic symptoms. The most significant classes, along with the minimum times between the last dose and the AEC challenge, are antihistamines (7 days), inhaled and/or intranasal corticosteroids (14 days); inhaled and/or intranasal cromolyn (14 days), and systemic corticosteroids and/or astemizole (30 days)18.
Limitations of the method 
The AEC challenge test is more expensive than direct provocation tests (nasal, conjunctival, and bronchial), which means it is not used in daily practice. AECs differ in terms of the sources of the allergen, the measurement of the distributed particles, and the trial time, which makes it very difficult to compare studies. When HDM allergens were used in the AEC, different material sources were applied: Der p 1 and Der f 1, Dp fecal material containing mainly Der p1 with a 20:1 predetermined ratio of Der p 1 to Der p 232, HDM allergen SQ 503 from body and feces containing Der p 1 and Der p 233, and Dp extracts. In the ALL-MED AEC, dried and purified Dp mite bodies, including Der p 1 and Der p 2, were used20. Therefore, unified standards should be introduced in the future so that outcomes can be compared among AECs.
The significance of the method with respect to existing/alternative methods 
AECs are a very useful but underrepresented in vivo method in allergy diagnostics. Additionally, as an assessment endpoint of clinical trials, AECs show significant superiority over classical &#34;in-field&#34; evaluations. It is of interest to examine the correlations among various clinical endpoints, particularly the similarity of subjective parameters assessed by patients (TNSS) and objective measures (acoustic rhinometry, PNIF, nasal discharge) gathered by the investigator, as an initial step in validating AEC results against those obtained in a &#34;field&#34; setting.
Future applications or directions of the method 
AECs offer a possible method for the stratification of patients into potential responders and non-responders. This method shows great promise for accelerating clinical developments in both the pharmacotherapy and immunotherapy of allergic diseases34. Thus, AECs have been one of the key areas of interest in recent years. AECs could be useful in long-term studies when it is not possible to evaluate the natural exposure due to low allergen counts.

Airborne allergies are becoming a growing social problem. Proper diagnosis, the assessment of the efficacy of allergen-specific immunotherapy (AIT), and understanding the pharmacotherapies are key points in addressing this issue. However, standardizing these procedures requires stable allergen concentrations, stable environmental conditions (e.g., humidity and temperature), and the capability to cause allergic signs in a repeatable manner. Allergen exposure chambers (AECs) provide stable environmental conditions, independent of external factors, and the concentration of dispersed allergen particles is well-controlled and stable during challenges in AECs1,2.
The allergen challenge test is the basis for diagnosing airborne allergies because it provides direct evidence of a specific allergen&#39;s clinical relevance to the symptoms and severity of the allergic disease. Classic allergic diagnostics include nasal, conjunctival, and bronchial provocations3,4,5. However, the allergen challenge test in an AEC appears to be the closest to natural allergen exposure6.
This study aims to present a safe and effective method of challenging participants with various airborne allergens in an AEC to trigger significant allergic symptoms corresponding to natural exposure. This method is suitable for the induction of pathological features of respiratory diseases, including allergic rhinitis and asthma, as an endpoint in the efficacy testing of AIT and might contribute to and accelerate the clinical development of pharmacological treatments2,3,7,8,9,10.
There are over a dozen AECs in the world11. However, the AECs are not comparable to each other because they are individually designed, use different types of allergens (e.g., house dust mite [HDM], birch pollen, grass pollen, cat, ragweed pollen, or Japanese cedar pollen), and have different measurement systems for the distributed particles12,13,14,15,16,17,18,19. Therefore, each AEC should be validated for individual allergens. AEC validation ensures that the proper concentration of the allergen is safe and that the symptoms are induced in patients. The ALL-MED AEC is validated for HDM allergens20.
The ALL-MED AEC is located at the Medical Research Institute in Wroclaw, Poland. The facility can comfortably accommodate 15-20 people during one trial. The facility consists of a room with an area of 12 m2, which is accessed by an airlock to prevent particles from the external environment from entering it. The equipment (seats, walls, etc.) is comprised of non-adhesive, accessible surfaces that can be washed, such as eco-leather, plastic, and metal. The chairs are movable, allowing for different setups. The viewing window and microphone communication allow for constant monitoring of the subjects (Figure 1). Particle accumulation is measured by a laser particle counter (LPC). The particles can be categorized into different ranges, including 0-20 &#181;m, 20-50 &#181;m, and 50-100 &#181;m, and the results are given in particles per cubic meter (p/m3) during a specified time unit (e.g., each minute). There are two accessory rooms next to the AEC, where patients undergo tests before entering the chamber. The rescue equipment consists of a defibrillator and other resuscitation devices housed in the facility. At least two healthcare workers, including a physician, are present during each challenge.

<table><tbody><tr><td>Allergen exposure chamber (AEC)</td><td>custom made</td><td>---</td><td>with the air supply duct (with HEPA filters) and allergen blew into the AEC through a computer-controlled feeder</td></tr><tr><td>Acoustic rhinometer&amp;nbsp;</td><td>GM Instruments (Irvine, UK)</td><td>A1 clinical/ reseach</td><td>with reusable plastic tips, contoured for the right and left nostrils</td></tr><tr><td>Air humidifier</td><td>Ohyama</td><td>SHM120D</td><td></td></tr><tr><td>Air quality meter</td><td>AZ Instrument</td><td>Green Eye VZ 7798</td><td>termometer, humidity and CO&lt;sub&gt;2&lt;/sub&gt; meter</td></tr><tr><td>Air-conditioning&amp;nbsp;</td><td>DeLonghi</td><td>CKP 20EB</td><td>temperature range 18 - 25 &amp;deg;C</td></tr><tr><td>Ceiling&amp;nbsp; fans</td><td>Argos&amp;nbsp;</td><td>Manhattan Ceiling Fan - 432/8317</td><td></td></tr><tr><td>Computer-controlled feeder station&amp;nbsp;</td><td>custom made</td><td>---</td><td>with control of &quot;injection length&quot;, &quot;break between injections &quot;, &amp;ldquo;air supply&amp;rdquo;</td></tr><tr><td>Disposable coveralls&amp;nbsp;</td><td>VWR (Radnor, Pennsylvania, United States)</td><td></td><td>with hoodies</td></tr><tr><td>Floor fans</td><td>AEG</td><td>TVL 5537, column</td><td></td></tr><tr><td>Graphing program</td><td>GraphPad Software Inc.</td><td>Graph Pad Prism, v. 9.4.0</td><td></td></tr><tr><td>House dust mite (HDM)</td><td>Allergopharma (Reinbek, Germany)</td><td>customized order</td><td>dried, purified &lt;em&gt;Dermatophagoides pteronyssinus (Dp)&lt;/em&gt; mite bodies, stored at 4 &amp;deg;C until use</td></tr><tr><td>Inspiratory flow meter&amp;nbsp;</td><td>Clement Clarke International Ltd. (Harlow, UK)</td><td>portable inspiratory flow meter</td><td>with the disposable mask (size M), measuring inspiratory flow between 30 - 370 L/ min</td></tr><tr><td>Laser particle counter (LPC)</td><td>Lighthouse Worldwide Solutions (USA)</td><td>SOLAIR Boulder Counte</td><td></td></tr><tr><td>Microphone system</td><td>Auna</td><td>VHF wireless microphone system</td><td></td></tr><tr><td>Peak flow matter (PFM)</td><td>CareFusion (Basingstoke, UK)&amp;nbsp;</td><td>MicroPeak with a standard range of 60 &amp;ndash; 900 L/ min</td><td>with the disposable paper tips</td></tr><tr><td>Remote controls for filling questionnaires</td><td>Turning Technologies</td><td>Pilot TT ResponseCard LT, SAP: G040602A010</td><td>a set of 32 remote controls for TT LT tests</td></tr><tr><td>Spirometer</td><td>Medizintechnik AG (Zurich, Switzerland)</td><td>EasyOne 2001, NDD</td><td>with the disposable paper tips; the spirometer should meet the ISO 26 782: 2009 standard; daily calibration of the spirometer is required</td></tr><tr><td>TV screen</td><td>Level</td><td>Level one 32&quot;</td><td></td></tr><tr><td>Vacuum</td><td>Siemens</td><td>extreme silencePower VSQ5X1230</td><td>with the HEPA filters</td></tr></tbody></table>

symptom assessment of patients with allergic rhinitis using an allergen exposure chamber

Allergen exposure chambers (AECs) are clinical facilities that allow the exposure of participants to allergenic and non-allergenic airborne particles. They provide stable particle concentrations under controlled environmental conditions. This is of great importance both for diagnostic purposes and for the monitoring of treatment effects. 
Here, a protocol and the technical prerequisites for performing a safe and effective allergen challenge in subjects sensitized to airborne allergens (i.e., house dust mite [HDM]) in the ALL-MED AEC are presented. With this method, triggering allergic symptoms corresponds to natural exposure. This can be used for an allergy diagnosis or as a plausible endpoint in clinical trials, particularly for allergen immunotherapy (AIT). A controlled environment (temperature, humidity, and carbon dioxide [CO2]) in the chamber must be maintained. Allergen particles must be dispersed evenly within the AEC at stable levels throughout the challenge. For this presentation, allergic rhinitis (AR) patients sensitive to HDM allergens were enrolled. AR symptoms were assessed by the following parameters: total nasal symptom score (TNSS), acoustic rhinometry (ARM), peak nasal inspiratory flow (PNIF), and nasal secretion weight. The safety of the procedure was assessed by the peak expiratory flow rate (PEFR) and the forced expiratory volume in the first second (FEV1). The allergic subjects developed symptoms within 120 min of the trial. On average, the most intense symptoms appeared after 60-90 min and, after reaching a plateau, remained stable until the end of the trial.

The AEC environment was monitored throughout the operation time for the number of allergens (p/m3), the temperature, the humidity, and the CO2 concentration (Figure 2). The HDM allergen levels were found to be stable (Figure 2A). Additionally, a trial in which no allergens were distributed is shown, with particles in the range of 0-20 &#181;m and a particle count maximum of 50 p/m3 (Figure 2A). There was an influx of particles originating from the participants entering the AEC, resulting in about 100 p/m3 for the 15 participants compared to an empty chamber. As a result, the values measured by the LPC during the trial included the target concentration with an influx of approximately 100 p/m3.
Paired data were compared with the Mann-Whitney U test. Values were considered statistically significant for all the tests with p &#60; 0.05. Statistical calculations were performed, and graphs were generated using a graphing program.
Two groups were included in the study to show the difference between positive and negative results: eight HDM allergic individuals with allergic rhinitis (AR) symptoms and seven healthy control (HC) individuals without allergies. Supplementary Table 1 presents the inclusion and exclusion criteria, as well as the participant characteristics. The participants were exposed to HDM at a concentration of 5,000 p/m3 for a duration of 120 min, according to the validation of the ALL-MED AEC20.
All participants underwent the following tests (ARM, PNIF, PERF, spirometry) and completed TNSS questionaries, and their nasal discharges were collected. TNSS and nasal discharge weight were significantly higher in AR individuals compared with the HC group (Figure 4A,B). The TNSS reached the peak values after 60 min of exposure and then plateaued (p &#60; 0.0001). Additionally, the nasal secretion weight was significantly higher in the AR group (p &#60; 0.0001). Impairment of the airway patency was noticed in the acoustic rhinometry. The MCA significantly decreased after the first measurement at 60 min when comparing the AR group to the HC group. From that point on until the end of the challenge, the values remained stable (p &#60; 0.001). This agreed with the PNIF measurements, for which a significant reduction was observed at the same concentrations (p &#60; 0.01) (Figure 4C,D).
The FEV1 and PEFR were measured during the AEC challenge (Figure 4E,F). Additionally, the participants measured their PEFR at home at 4 h and 24 h after the challenge and returned the results by mail. The values were within the normal range and remained stable during the challenge and for up to 24 h thereafter. No statistically significant differences were found between the allergic subjects with AR and the HCs, which suggests that the HDM allergen exposure had no effects on lung function in either group.
<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/64801/64801fig01.jpg" /> 
Figure 1: Schematic layout of the AEC. Participants enter through the airlock. The particles are distributed through the system of air vents by a computer-controlled feeder. The AEC conditions (particle concentration, CO2 concentration, humidity, and temperature) are constantly monitored by an LPC. The participants are monitored by the window and voice connection. Abbreviations: AEC = allergen exposure chamber; CO2 = carbon dioxide; LPC = laser particle counter. <a href="https://www.jove.com/files/ftp_upload/64801/64801fig01large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 2" class="xfigimg" src="/files/ftp_upload/64801/64801fig02.jpg" /> 
Figure 2: Representative results of the stability of the environment in the AEC during the trial. (A) The particle concentration was assessed and found to be in the range of 0-20 &#181;m by LPC. The target value for the concentration of HDM allergens was 5,000 p/m3. For comparison, a trial where no allergen was used is shown. (B) Humidity, (C) CO2 concentration, and (D) and temperature are shown. Abbreviations: &#176;C = degrees Celsius; CO2 = carbon dioxide; HDM = house dust mite; LPC = laser particle counter; m = meter; min = minute(s); p = particles; ppm = parts per million. <a href="https://www.jove.com/files/ftp_upload/64801/64801fig02large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 3" class="xfigimg" src="/files/ftp_upload/64801/64801fig03.jpg" /> 
Figure 3:&#160;List of tests to be performed during the AEC challenge, with time points (for each participant). To ensure the timely execution of individual tests, the participants should enter the AEC every 10 min. As a result, the test for each participant will be conducted at different real times. Furthermore, the time shift allows the staff to help the participants during testing. Abbreviations: AEC = allergen exposure chamber; ARM = acoustic rhinometry; FEV1 = forced expiratory volume in the first second; PEFR = peak expiratory flow rate; PNIF = peak nasal inspiratory flow; TNSS = total nasal symptom score. <a href="https://www.jove.com/files/ftp_upload/64801/64801fig03large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 4" class="xfigimg" src="/files/ftp_upload/64801/64801fig04v2.jpg" /> 
Figure 4: Representative results of different endpoints during the AEC challenge in patients with AR (red bars) and HCs (blue bars). HMD-triggered allergy subjects (with AR) and HC, including eight and seven participants, respectively, were exposed to HDM allergen concentrations of 5,000 p/m3 in the AEC. (A) Nasal secretion weight, (B) nasal symptoms, (C) MCA in acoustic rhinometry, (D) PNIF, (E) PEFR, and (F) FEV1 were evaluated. The results are presented as individual replicates with the mean value. Abbreviations: AEC = allergen exposure chamber; AR = allergic rhinitis; FEV1 = forced expiratory volume in the first second; HC = healthy controls; HDM = house dust mite; g = gram(s); MCA = minimal cross-sectional area; p = particles; PEFR = peak expiratory flow rate; PNIF = peak nasal inspiratory flow; TNSS = total nasal symptom score. <a href="https://www.jove.com/files/ftp_upload/64801/64801fig04largev2.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>symptom</td>
			<td>question displayed on a TV screen</td>
			<td>TNSS score for each symptom</td>
		</tr>
		<tr>
			<td>rhinorrhea</td>
			<td>Rate how your runny nose has been at this moment</td>
			<td>0 = none (symptom completely absent)</td>
		</tr>
		<tr>
			<td>nasal obstruction</td>
			<td>Rate how your nasal congestion has been at this moment</td>
			<td>1 = mild (symptom present, but not distressing)</td>
		</tr>
		<tr>
			<td>sneezing</td>
			<td>Rate how your sneezing has been at this moment</td>
			<td>2 = moderate (symptom distressing, but tolerable)</td>
		</tr>
		<tr>
			<td>nasal itching</td>
			<td>Rate how your nasal itching has been at this moment</td>
			<td>3 = severe (symptom hard tolerable, maximum intensity)</td>
		</tr>
		<tr>
			<td></td>
			<td></td>
			<td>0 - 12 points total</td>
		</tr>
	</tbody>
</table>
Table 1: Symptoms and score method for TNSS. A rating system was used by the participants to evaluate four symptoms. The survey results are presented as one value-a total score for the four questions for a given time (before the trial and every 30 min of the trial). Abbreviation: TNSS = total nasal symptom score.
Supplementary Table 1: Inclusion and exclusion criteria for the study and characteristics of the participants enrolled in the study. Eight patients with AR symptoms, triggered by HDM, and seven patients with no symptoms (HCs). Abbreviations: AR = allergic rhinitis; Df = Dermatophagoides farinae; Dp = Dermatophagoides pteronyssinus; F = female; HC = healthy control; HDM = house dust mite; kU/L = kilo units/liter; M = male; md = mean diameter; sIgE = specific immunoglobulin E; SPT = skin prick test. <a href="https://www.jove.com/files/ftp_upload/64801/64801_SuppTable1.xlsx" target="_blank">Please click here to download this File.</a>

Watch this Scientific Journal Video about Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber at JoVE.com

Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber

A protocol for conducting a challenge in an allergen exposure chamber (AEC) facility is presented. AECs have proven to be safe and effective tools for the induction of allergic symptoms or as an endpoint in the efficacy testing of allergen immunotherapy due to their ability to maintain stable particle concentrations and environmental conditions.

Allergen exposure chambers (AECs) are clinical facilities that allow the exposure of participants to allergenic and non-allergenic airborne particles. ...

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Immunology and Infection

2.2K Views.  Wroclaw Medical University. Allergenenblootstellingskamers maken gecontroleerde blootstelling aan allergene en niet-allergene stedelijke deeltjes in een besloten omgeving mogelijk. Ze worden gebruikt voor gecontroleerde uitdagingen en het nabootsen van natuurlijke blootstelling. De allergeenblootstellingskamer vergemakkelijkt de observatie van verschillende klinische eindpunten, met name objectieve metingen zoals neusobstructie of secretie, en subjectieve parameters die door de patiënt worden geëvalueerd.Allerg...

Video: Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber

Murine Model of Allergen Induced Asthma

Asthma is a major cause of morbidity and mortality, affecting some 300 million people throughout the world.1 More than 8% of the US population has asthma, with the prevalence increasing.2 As with other diseases, animal models of allergic airway disease greatly facilitate understanding of the underlying pathophysiology, help identify potential therapeutic targets, and allow preclinical testing of possible new therapies. Models of allergic airway disease have been developed in several animal species, but murine models are particularly attractive due to the low cost, ready availability, and well-characterized immune systems of these animals.3 Availability of a variety of transgenic strains further increases the attractiveness of these models.4 Here we describe two murine models of allergic airway disease, both employing ovalbumin as the antigen. Following initial sensitization by intraperitoneal injection, one model delivers the antigen challenge by nebulization, the other by intratracheal delivery. These two models offer complementary advantages, with each mimicking the major features of human asthma.5
The major features of acute asthma include an exaggerated airway response to stimuli such as methacholine (airway hyperresponsiveness; AHR) and eosinophil-rich airway inflammation. These are also prominent effects of allergen challenge in our murine models,5,6 and we describe techniques for measuring them and thus evaluating the effects of experimental manipulation. Specifically, we describe both invasive7 and non-invasive8 techniques for measuring airway hyperresponsiveness as well as methods for assessing infiltration of inflammatory cells into the airways and the lung. Airway inflammatory cells are collected by bronchoalveolar lavage while lung histopathology is used to assess markers of inflammation throughout the organ. These techniques provide powerful tools for studying asthma in ways that would not be possible in humans.

Experimental mouse models of allergic asthma offer new possibilities for studying disease pathogenesis and developing new therapeutics. These models are well suited to measuring factors governing the allergic immune response, airway inflammation, and pulmonary pathophysiology.

Asthma is a major cause of morbidity and mortality, affecting some 300 million people throughout the world.1 More than 8% of the US population has asthma, ...

Development of a Nose-only Inhalation Toxicity Test Chamber That Provides Four Exposure Concentrations of Nano-sized Particles

Using a numerical analysis based on computerized fluid dynamics, a nose-only inhalation toxicity chamber with four different exposure concentrations is designed and validated for flow field uniformity and cross-contamination among the exposure ports for each concentration. The designed flow field values are compared with the measured values from exposure ports located horizontally and vertically. For this purpose, nanoscale sodium chloride particles are generated as test particles and introduced to the inhalation chamber to evaluate the cross-contamination and concentration maintenance among the chambers, for each concentration group. The results indicate that the designed multiconcentration inhalation chamber can be used in animal inhalation toxicity testing without cross-contamination among concentration groups. Moreover, the designed multiconcentration inhalation toxicity chamber can also be converted to a single-concentration inhalation chamber. Further testing with gas, organic vapor, or non-nanoscale particles will ensure the use of the chamber in the inhalation testing of other test articles.

A nose-only inhalation toxicity chamber capable of testing inhalation toxicity at four different exposure concentrations was designed and validated for flow field uniformity and cross-contamination between the exposure ports for each concentration. Here, we present a protocol to confirm that the designed chamber is effective for inhalation toxicity testing.

Using a numerical analysis based on computerized fluid dynamics, a nose-only inhalation toxicity chamber with four different exposure concentrations is ...

Engineering

A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice

Airway hyperreactivity (AHR) measurements and bronchoalveolar lavage (BAL) fluid sampling are essential to experimental asthma models, but repeated procedures to obtain such measurements in the same animal are generally not feasible.  Here, we demonstrate protocols for obtaining from mice repeated measurements of AHR and bronchoalveolar lavage fluid samples.  Mice were challenged intranasally seven times over 14 days with a potent allergen or sham treated.  Prior to the initial challenge, and within 24 hours following each intranasal challenge, the same animals were anesthetized, orally intubated and mechanically ventilated.  AHR, assessed by comparing dose response curves of respiratory system resistance (RRS) induced by increasing intravenous doses of acetylcholine (Ach) chloride between sham and allergen-challenged animals, were determined.  Afterwards, and via the same intubation, the left lung was lavaged so that differential enumeration of airway cells could be performed.  These studies reveal that repeated measurements of AHR and BAL fluid collection are possible from the same animals and that maximal airway hyperresponsiveness and airway eosinophilia are achieved within 7-10 days of initiating allergen challenge.  This novel technique significantly reduces the number of mice required for longitudinal experimentation and is applicable to diverse rodent species, disease models and airway physiology instruments.  

Repeated measurements of rodent respiratory physiology and sampling of airway inflammatory cells are desirable, but generally not feasible. Here we describe a repeatable method for orally intubating mice that permits repeated measurements of airway hyperreactivity and sampling of airway inflammatory cells.

Airway hyperreactivity (AHR) measurements and bronchoalveolar lavage (BAL) fluid sampling are essential to experimental asthma models, but repeated ...

Biology

A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma

Sensitization to grass pollen imposes a global risk for allergic airway diseases. Although prevention relies on local investigation of the pollen allergens, data on this topic are limited in southern China. Any available data were obtained by self-report questionnaires, skin prick tests, and total or specific IgE tests using crude extracts. For many reasons, these methods are unreliable. Serum sIgE reactivity to Bermuda grass, Timothy grass, and Humulus scandens allergens in a cohort of patients from Greater Guangzhou (southern China&#39;s largest city and its outskirts) with allergic rhinitis and/or asthma were examined using a fully-automated immunoassay analyzer as a component-resolved diagnostics (CRD) tool.
For the first time, a considerably high prevalence of Bermuda grass sIgE positivity was demonstrated in Chinese southerners with allergic rhinitis and/or asthma. In these patients, a subtle prevalence of sensitization to Timothy grass and Humulus scandens was also noted, which may arise from cross-reactivity, as the latter two are not common in the region. This was also supported by the detection of allergen components.
Fully-automated immunoassay analyzers may offer satisfactory consistency between regions, laboratories, and institutions and over time. The automaticity of the instrument may enable a standardized detection that would not have been readily revealed before the advent of CRD.
This is a study that uses a CRD approach to investigate sensitization to grass pollen allergens in southern China. It adds to current evidence in the literature. Future studies are needed to validate these findings. However, although CRD is a useful tool, the findings made with the fully-automated immunoassay analyzer should not substitute for other laboratory investigations, clinical evaluations, and physician expertise.

This work describes a protocol that uses a component-resolved approach to study sensitization to grass pollen allergens in a cohort of patients from southern China with allergic rhinitis and/or asthma.

Sensitization to grass pollen imposes a global risk for allergic airway diseases. Although prevention relies on local investigation of the pollen ...

Medicine

Absorption of Nasal and Bronchial Fluids: Precision Sampling of the Human Respiratory Mucosa and Laboratory Processing of Samples

The methods of nasal absorption (NA) and bronchial absorption (BA) use synthetic absorptive matrices (SAM) to absorb the mucosal lining fluid (MLF) of the human respiratory tract. NA is a non-invasive technique which absorbs fluid from the inferior turbinate, and causes minimal discomfort. NA has yielded reproducible results with the ability to frequently repeat sampling of the upper airway. 
By comparison, alternative methods of sampling the respiratory mucosa, such as nasopharyngeal aspiration (NPA) and conventional swabbing, are more invasive and may result in greater data variability. Other methods have limitations, for instance, biopsies and bronchial procedures are invasive, sputum contains many dead and dying cells and requires liquefaction, exhaled breath condensate (EBC) contains water and saliva, and lavage samples are dilute and variable.
BA can be performed through the working channel of a bronchoscope in clinic. Sampling is well tolerated and can be conducted at multiple sites in the airway. BA results in MLF samples being less dilute than bronchoalveolar lavage (BAL) samples.
This article demonstrates the techniques of NA and BA, as well as the laboratory processing of the resulting samples, which can be tailored to the desired downstream biomarker being measured. These absorption techniques are useful alternatives to the conventional sampling techniques used in clinical respiratory research.

This manuscript describes the use of nasal and bronchial absorption techniques, specifically using synthetic absorptive matrices (SAM) to sample the mucosal lining fluid (MLF) of the upper and lower airway. These methods provide better standardization and tolerability than existing respiratory sampling techniques.

The methods of nasal absorption (NA) and bronchial absorption (BA) use synthetic absorptive matrices (SAM) to absorb the mucosal lining fluid (MLF) of the ...

Assessment of Respiratory Function in Conscious Mice by Double-chamber Plethysmography

Air volume changes created by a conscious subject breathing spontaneously within a body box are at the basis of plethysmography, a technique used to non-invasively assess some features of the respiratory function in humans as well as in laboratory animals. The present article focuses on the application of the double-chamber plethysmography (DCP) in small animals. It provides background information on the methodology as well as a detailed step-by-step procedure to successfully assess respiratory function in conscious, spontaneously breathing animals in a non-invasive manner. The DCP can be used to monitor the respiratory function of multiple animals in parallel, as well as to identify changes induced by aerosolized substances over a chosen time period and in a repeated manner. Experiments on control and allergic mice are used herein to demonstrate the utility of the technique, explain the associated outcome parameters, as well as to discuss the related advantages and shortcomings. Overall, the DCP provides valid and theoretically sound readouts that can be trusted to evaluate the respiratory function of conscious small animals both at baseline and after challenges with aerosolized substances.

The objective of the present article is to provide a detailed description of the recommended procedures to evaluate respiratory function in conscious mice by double-chamber plethysmography.

Air volume changes created by a conscious subject breathing spontaneously within a body box are at the basis of plethysmography, a technique used to ...

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)

Allergic disease is common in both adults and children. Identification of the causative allergens is significant in disease management and prevention. However, a specific immunoglobulin E (IgE) measurement system with a high price-performance ratio is lacking in mainland China, especially in the primary care hospitals. This paper describes the principle and operation procedures of using a microfluidic cartridge-based chemiluminescence system to detect allergen-specific IgE in serum. The results were compared with those from ImmunoCAP (System 1), the industrial standard, and the reproducibility of the system to detect patients sensitized to common allergens is evaluated. The results showed that in comparison with ImmunoCAP (System 1), the BioIC System (System 2) has good precision and sensitivity in detecting serum-specific IgE against various inhalant and food allergens but with a significantly lower cost. It can serve as a good alternative to System 1 in primary care hospitals in mainland China who have lower financial affordability.

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

This paper presents a protocol for detecting serum-specific immunoglobulin E with a microfluidic cartridge-based chemiluminescence system and the evaluation of its usefulness in allergy diagnoses.

Allergic disease is common in both adults and children. Identification of the causative allergens is significant in disease management and prevention. ...

Basophil Activation Test for Allergy Diagnosis

The basophil activation test (BAT) is a complementary in vitro diagnostic test that can be used in addition to clinical history, skin test (ST), and specific IgE (sIgE) determination in the evaluation of IgE-mediated allergic reactions to food, insect venom, drugs, as well as some forms of chronic urticaria. However, the role of this technique in the diagnostic algorithms is highly variable and not well determined.
BAT is based on the determination of basophil response to allergen/drug cross-linking IgE activation through the measurement of activation markers (such as CD63, CD203c) by flow cytometry. This test can be a useful and complementary tool to avoid controlled challenge tests&#160;to confirm allergy diagnosis, especially in subjects experiencing severe life-threatening reactions. In general, the performance of BAT should be considered if i) the allergen/drug produces false positive results in ST; ii) there is no allergen/drug source to use for ST or sIgE determination; iii) there is discordance between patient history and ST or sIgE determination; iv) symptoms suggest that ST may result in systemic response; v) before considering a CCT to confirm the culprit allergen/drug. The main limitations of the test are related to non-optimal sensitivity, especially in drug allergy, the need to perform the test no longer than 24 h after sample extraction, and the lack of standardization between laboratories in terms of procedures, concentrations, and cell markers.

The basophil activation test is a complementary in vitro diagnostic test for the evaluation of IgE-mediated allergic reactions based on the detection of basophil activation in the presence of a specific stimulus through the measure of activation markers by flow cytometry.

The basophil activation test (BAT) is a complementary in vitro diagnostic test that can be used in addition to clinical history, skin test (ST), and ...

Multicolor Immunofluorescence of the Nasal Tissue in an Allergic Rhinitis Rat Model

Immunofluorescent Labeling in Nasal Mucosa Tissue Sections of Allergic Rhinitis Rats via Multicolor Immunoassay

Allergic rhinitis (AR) is a chronic, non-infectious inflammatory disease of the nasal mucosa, primarily mediated by specific immunoglobulin E (IgE), affecting approximately 10%-20% of the world's population. While immunofluorescence (IF) staining has long been a standard technique for detecting disease-specific protein expression, conventional IF techniques are limited in their ability to detect the expression levels of three or more proteins in the same sample. Consequently, multicolor IF techniques have been developed in recent years, which allow the simultaneous labeling of multiple targets in cells or tissues.
This protocol provides a comprehensive overview of the process for establishing a rat model of AR, obtaining nasal mucosal samples, and the technical procedures for multicolor immunofluorescence. All rats in the AR group exhibited typical symptoms such as sneezing, a runny nose, and an itchy nose, with behavioral observations scoring &#8805;5 points. Hematoxylin and eosin (H&amp;E) staining revealed increased inflammatory cell counts and disrupted nasal mucosal integrity in the AR group. Multicolor immunofluorescence (mIF) demonstrated increased expression of ROR&#947;t and TICAM-1, while Foxp3 expression decreased in the nasal mucosa tissue of AR rats.

Author Spotlight: Advancing Allergic Rhinitis Research with Multicolor Immunofluorescence

This protocol describes a multicolor immunofluorescence technique for evaluating the rat model of allergic rhinitis.

Allergic rhinitis (AR) is a chronic, non-infectious inflammatory disease of the nasal mucosa, primarily mediated by specific immunoglobulin E (IgE), ...

Evaluating Acupoint Catgut Embedding Therapy for Allergic Rhinitis Management

Acupoint Catgut Embedding Therapy in Traditional Chinese Medicine for Managing Allergic Rhinitis

Acupuncture Point Embedding Therapy is a comprehensive treatment method that combines modern biomaterials with acupuncture techniques. Acupoint catgut embedding (ACE) not only avoids the adverse reactions associated with Western medicine but also incorporates the benefits of traditional acupuncture, extending the duration of its therapeutic effects. Allergic rhinitis (AR) is a condition known for its challenging treatment and tendency to persist. This randomized controlled trial included 128 AR patients to evaluate the effectiveness of ACE for AR management. Data were collected at baseline, four weeks after treatment, and eight weeks after treatment. Baseline analysis was performed, and the visual analog scale (VAS) was used as a clinical observation index for this method. The rhinoconjunctivitis quality of life questionnaire (RQLQ) served as a quality of life index. Specific IgE (sIgE), IL-4, IL-10, and IL-12 were used as laboratory observation indices. The results of the experiment validate the clinical effectiveness of ACE in treating AR. Although this technique is efficient and commonly used in clinics, its invasive nature poses risks of hospital-acquired infections. Based on this experiment, this protocol refines traditional techniques, providing detailed instructions on patient preparation, operation techniques, and postoperative care to ensure the safe and effective administration of therapy. By standardizing this therapy, ACE is anticipated to become an important non-drug treatment option for alleviating symptoms in AR patients, thereby significantly enhancing their quality of life.

This protocol details the procedure for acupoint burrowing and demonstrates its effectiveness in treating allergic rhinitis.

Acupuncture Point Embedding Therapy is a comprehensive treatment method that combines modern biomaterials with acupuncture techniques. Acupoint catgut ...

Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber

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