Barrier Functional Integrity Recording on bEnd.3 Vascular Endothelial Cells via Transendothelial Electrical Resistance Detection

2.2K Views

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09:03 min

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September 29th, 2023

DOI :

10.3791/65938-v

September 29th, 2023

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Fuhan Fan¹, Hong Jiang¹, Ya Hou¹, Yi Zhang¹^,²^,³, Qipeng Zhao⁴, Yong Zeng¹^,², Xianli Meng¹^,²^,⁵, Xiaobo Wang²

¹School of Pharmacy/School of Modern Chinese Medicine Industry, Chengdu University of Traditional Chinese Medicine, ²Meishan Hospital of Chengdu University of Traditional Chinese Medicine, ³School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, ⁴School of Pharmacy, Ningxia Medical University, ⁵Innovative Institute of Chinese Medicine and Pharmacy/Academy for Interdiscipline, Chengdu University of Traditional Chinese Medicine

Transcript

Summary

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Barrier Functional Integrity

Transendothelial Electrical Resistance

Neurological Disorders

Tight Junction Proteins

In Vitro Models

Central Nervous System

Cell Barrier Function

Pharmacological Treatments

Chapters in this video

0:00

Introduction

1:17

Culture of bEnd·3 Cells and Cell Viability After CoCl₂ Treatment

3:47

In Vitro Blood‐Brain Barrier Model Assembly for Measurement of TEER

7:24

Barrier Destruction and Statistical Analysis After TEER Measurement in an In Vitro Blood‐Brain Barrier Model

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