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Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish

DOI :

10.3791/50312-v

April 30th, 2013

April 30th, 2013

13,259 Views

1Department of Physiology, University of California, Los Angeles

In this video, we will demonstrate how to record electrical activity from identified single neurons in a whole brain preparation, which preserves complex neural circuits. We use transgenic fish in which gonadotropin-releasing hormone (GnRH) neurons are genetically tagged with a fluorescent protein for identification in the intact brain preparation.

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Electrical Activity

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