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National Institute of Optics-National Research Council, Italy
2 ARTICLES PUBLISHED IN JoVE
Biology
Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
Carina Monico 1,2, Gionata Belcastro 1, Francesco Vanzi 1,3, Francesco S. Pavone 1,4,5,6, Marco Capitanio 1,4
1LENS - European Laboratory for Non-linear Spectroscopy, University of Florence, 2Chemistry Research Laboratory, University of Oxford, 3Department of Biology, University of Florence, 4Department of Physics and Astronomy, University of Florence, 5National Institute of Optics-National Research Council, Italy, 6International Center of Computational Neurophotonics
Here we describe the instrumentation and methods for detecting single fluorescently-labeled protein molecules interacting with a single DNA molecule suspended between two optically trapped microspheres.
Biology
Mesoscopic Optical Imaging of Whole Mouse Heart
Francesco Giardini *1, Erica Lazzeri *1, Camilla Olianti *1, Giada Beconi 1, Irene Costantini 1,2, Ludovico Silvestri 1,3,4, Elisabetta Cerbai 1,5, Francesco S. Pavone 1,3,4, Leonardo Sacconi 1,3,6
1European Laboratory for Non-Linear Spectroscopy, 2Department of Biology, University of Florence, 3National Institute of Optics, National Research Council, 4Department of Physics and Astronomy, University of Florence, 5Department of Neurosciences, Psychology, Drugs and Child Health, University of Florence, 6Institute for Experimental Cardiovascular Medicine, Faculty of Medicine, University of Freiburg
We report a method for mesoscopic reconstruction of the whole mouse heart by combining new advancements in tissue transformation and staining with the development of an axially scanned light-sheet microscope.
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