National Institute of Optics-National Research Council, Italy

2 ARTICLES PUBLISHED IN JoVE

Biology

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Carina Monico^1,2, Gionata Belcastro¹, Francesco Vanzi^1,3, Francesco S. Pavone^1,4,5,6, Marco Capitanio^1,4

¹LENS - European Laboratory for Non-linear Spectroscopy, University of Florence, ²Chemistry Research Laboratory, University of Oxford, ³Department of Biology, University of Florence, ⁴Department of Physics and Astronomy, University of Florence, ⁵National Institute of Optics-National Research Council, Italy, ⁶International Center of Computational Neurophotonics

Here we describe the instrumentation and methods for detecting single fluorescently-labeled protein molecules interacting with a single DNA molecule suspended between two optically trapped microspheres.

Biology

Mesoscopic Optical Imaging of Whole Mouse Heart

Francesco Giardini^*1, Erica Lazzeri^*1, Camilla Olianti^*1, Giada Beconi¹, Irene Costantini^1,2, Ludovico Silvestri^1,3,4, Elisabetta Cerbai^1,5, Francesco S. Pavone^1,3,4, Leonardo Sacconi^1,3,6

¹European Laboratory for Non-Linear Spectroscopy, ²Department of Biology, University of Florence, ³National Institute of Optics, National Research Council, ⁴Department of Physics and Astronomy, University of Florence, ⁵Department of Neurosciences, Psychology, Drugs and Child Health, University of Florence, ⁶Institute for Experimental Cardiovascular Medicine, Faculty of Medicine, University of Freiburg

We report a method for mesoscopic reconstruction of the whole mouse heart by combining new advancements in tissue transformation and staining with the development of an axially scanned light-sheet microscope.