Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow CytometrySusann Hartung 1,2, Christopher Rauh 2, Sarah Böttcher 1, Mai Thi Ngoc Hoang 1,2, Susanne Jahreis 1,2, Silke Rummler 3, Andreas Hochhaus 2, Marie von Lilienfeld-Toal 1,2
1Infections in Hematology and Oncology, Leibniz Institute for Infection Biology and Natural Product Research, 2Department for Hematology and Medical Oncology, Jena University Hospital, 3Institute for Transfusion Medicine, Jena University Hospital
This protocol provides a fast and reliable method to quantitatively measure phagocytosis of Aspergillus fumigatus conidia by human primary phagocytes using flow cytometry and to discriminate phagocytosis of conidia from mere adhesion to leukocytes.
