Etablissement Français du Sang Auvergne-Rhône-Alpes

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Generation of Human Regulatory T Cell Clones

¹Department of Medicine, Division of Rheumatology, New York University School of Medicine, ²Immunobiology and Immunotherapy in Chronic Diseases, Institute for Advanced Biosciences, Université Grenoble Alpes, Inserm U1209/CNRS UMR 5309, ³Etablissement Français du Sang Auvergne-Rhône-Alpes

This protocol describes the cloning and expansion of human regulatory T cells for the generation of ultra-high purity viable human Treg with stable demethylation at the Treg-specific demethylated region (TSDR) and Treg-specific phenotypic features.