Generation of Human Regulatory T Cell ClonesJohannes Nowatzky 1, Olivier Manches 2,3
1Department of Medicine, Division of Rheumatology, New York University School of Medicine, 2Immunobiology and Immunotherapy in Chronic Diseases, Institute for Advanced Biosciences, Université Grenoble Alpes, Inserm U1209/CNRS UMR 5309, 3Etablissement Français du Sang Auvergne-Rhône-Alpes
This protocol describes the cloning and expansion of human regulatory T cells for the generation of ultra-high purity viable human Treg with stable demethylation at the Treg-specific demethylated region (TSDR) and Treg-specific phenotypic features.
