Counting and Determining the Viability of Cultured Cells

Podsumowanie

Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. In this video, we demonstrate how cells are counted using a hemacytometer.

Streszczenie

Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. A hemacytometer is a thick glass slide with a central area designed as a counting chamber. Cell suspension is applied to a defined area and counted so cell density can be calculated.

Protokół

The complete text protocol for this experimental approach is available in Current Protocols in Cell Biology.

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