assembling magnet sets and conducting multilayered cell patterning using magnetic fields

A Novel Ink-Free, Label-Free Magnetic-Archimedes Cell Patterning Method

Cell patterning is evolving into an intuitive and powerful technology for in vitro studies1. By manipulating cell positions in culture plates, it provides solutions for a variety of experiments, including cell migration2, biomimetic multicellular co-culture3, organoid assembly4, biomaterial studies5, and more. In most situations, an ink-free, label-free method is preferred for cell patterning because it offers ease of operation and high cell viability for subsequent investigations.
The Mag-Arch effect is a physical phenomenon wherein diamagnetic objects in paramagnetic liquids tend to move toward regions with weak magnetic fields6. Living cells are naturally diamagnetic, while cell culture media can be made paramagnetic by adding soluble paramagnetic elements, such as gadopentetate dimeglumine (Gd-DTPA), commonly used intravenously in nuclear magnetic resonance imaging as a contrast agent7. Consequently, cells are expected to be repelled by the surrounding paramagnetic medium and move toward regions where magnetic fields are weaker8. A patterned magnetic field can be easily generated using a set of neodymium magnets. Ideally, cell patterns are assembled in opposition to the magnet patterns. Technically, this is defined as a label-free method because the only additional reagent, Gd-DTPA, remains in the extracellular environment and does not bind to cells. Thus, potential influences on subsequent cell culture can be easily avoided by replacing the culture medium. Compared to other methods1,3,9,10, the Mag-Arch-based strategy does not require bio-ink components or the application of specific particles to positively label the cells. Furthermore, it has been shown to work on multiple substrates for cell adhesion and is capable of high-throughput cell patterning4.
This article presents a detailed protocol for cell patterning using the Mag-Arch-based method, covering everything from device fabrication to adjusting the cell pattern. In addition to the patterns we have demonstrated, users can easily create various cell patterns using magnets and Gd-DTPA solution. Furthermore, protocols for assembling complex co-culture patterns and manipulating cells in enclosed microfluidic chips, are also provided.

Author Spotlight: Magnetic-Based Cell Patterning Method for High-Throughput Biomedical Applications 

Cell Patterning Using Magnetic-Archimedes Strategy

Cell patterning is intuitive and powerful technology for in vitro studies. Our research aims to present a versatile, user-friendly, and inexpensive cell pattern method, which could be replicated in most biomedical laboratories. Compared to other methods, our protocol doesn't require specialized skills and equipment.Also, it advances parallel to characters of ink-free, label-free, substrate-independent, and the ability for high-throughput patterning. The protocol requires only two necessities:magnets and the gadolinium-based contrast agents, which are both easy to get at low prices. The magnetic-based cell pattern method provides convenient solutions for the first step of a variety of experiments, including cell migration, biomimetic multicellular co-culture, organoid assembly, biomaterial studies, and more.

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Assembling Magnet Sets and Conducting Multilayered Cell Patterning Using Magnetic Fields  

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Research

JoVE Journal

Bioengineering

203 Views.  Zhejiang University School of Medicine. Assembling Magnet Sets and Conducting Multilayered Cell Patterning Using Magnetic Fields