Methods Article

Analysis of Protein Complex Formation at Micromolar Concentrations by Coupling Microfluidics with Mass Photometry

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06:39 min

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January 26th, 2024

DOI :

10.3791/65772-v

January 26th, 2024

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Myndert Claasen¹, Zornitsa Kofinova¹, Matteo Contino², Weston B. Struwe³^,⁴

¹Refeyn Ltd., ²Adaptix Ltd., ³Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford, ⁴The Kavli Institute for Nanoscience Discovery, University of Oxford

Transkrypcja

Podsumowanie

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Protein Complex Formation

Micromolar Concentrations

Mass Photometry

Microfluidics

Low Affinity Interactions

Protein Interactions

Biomolecular Interactions

Transient Interactions

Dilution Technique

Measurement Method

Rozdziały w tym wideo

0:00

Introduction

1:06

Measuring Biomolecular Interactions with Weaker Affinities Using Mass Photometry and Microfluidics

4:32

Analysis of the Microfluidics Coupled Mass Photometry Data to Investigate Low‐Affinity Protein‐Protein Interactions

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