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Inducing a Spinal Cord Contusion in a Mouse Model Using a Spinal Cord Injury Coaxial Platform

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Transkrypcja

Begin with an anesthetized mouse on the spinal cord injury coaxial platform, with the exposed 9th thoracic or T9 vertebrae level spinal cord. 

Using the platform’s stabilizer forceps, secure the vertebrae and prevent movement.

Place the tubular sleeve containing the impactor tip with a weight secured with a pull pin over the exposed spinal cord.

Adjust the tip position to accurately target the T9 spinal cord level.

Lower the sleeve until the impactor tip reaches the predetermined height above the spinal cord. 

Release the pull pin to drop the weight onto the impactor tip, striking the spinal cord with controlled impact. 

The impact delivers a blunt force that causes tissue compression, leading to structural damage in axons and blood vessels. 

This results in a contusion or injury from tissue compression. 

Remove the impactor. Tissue discoloration and swelling indicate a successful spinal cord contusion.

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