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Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs

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21:38 min

October 1st, 2007

DOI :

10.3791/308-v

October 1st, 2007


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Immunohistochemistry

Rozdziały w tym wideo

13:21

Preparing and Reacting Slides with DAB Substrate Reagent

0:49

Dewaxing and Rehydrating Paraffin Sections

0:02

Title

0:06

Introduction

5:14

Blocking Endogenous Peroxidase Activity and Nonspecific Binding of

11:39

Preparing and Incubating Slides in the ABC Reagent

3:20

Unmasking Antigen with Heat-Induced Antigen Retrieval

8:17

Adding Primary and Secondary Antibodies

16:10

Counterstaining and Mounting the Slides

20:46

Visualizing Anti-Tyrosine Hydroxylase Slides

21:07

Conclusion

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