The Neuroblast Assay: An Assay for the Generation and Enrichment of Neuronal Progenitor Cells from Differentiating Neural Stem Cell Progeny Using Flow Cytometry

Please note that all translations are automatically generated. Click here for the English version.

15.4K Views

•

15:40 min

•

April 22nd, 2012

DOI :

10.3791/3712-v

April 22nd, 2012

•

Hassan Azari¹^,², Sharareh Sharififar¹, Jeff M. Fortin¹, Brent A. Reynolds¹

¹Department of Neurosurgery, The University of Florida, ²Laboratory for Stem Cell Research, Department of Anatomical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran

Podsumowanie

Rozdziały w tym wideo

0:02

Title

1:46

Starting Neuroblast Assay Culture

4:07

Switching Neuroblast Assay Medium

4:55

Cell Preparation for Flow Cytometry

6:42

Staining for PSA-NCAM

13:12

Representative Results

14:34

Conclusion

8:31

Isolation of Neuronal Progenitor Cells Using Flow Cytometry

11:34

Plating Sorted Cells

Powiązane Filmy

13:21

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

26.8K Views

11:54

Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay

24.6K Views

06:31

Neural-Colony Forming Cell Assay: An Assay To Discriminate Bona Fide Neural Stem Cells from Neural Progenitor Cells

22.0K Views

04:27

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae

12.0K Views

12:48

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

17.1K Views

10:33

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

14.1K Views

13:05

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

52.0K Views

09:27

Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics

14.1K Views

10:32

Enumeration of Neural Stem Cells Using Clonal Assays

8.2K Views

10:45

Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays

9.8K Views