The authors would like to thank the following for funding this work: generous start-up funds from the Department of Biomedical Engineering in Washington University, a McDonnell Center for Systems Neuroscience grant, a Office of Naval Research grant (Grant#: N000141210089) to B.R.

1. Odor Preparation and Delivery
<ol>
 <li>Dilute odor solutions in mineral oil by volume to achieve the desired concentration level. Store a 20 ml mixture of mineral oil and the odorant in a 60 ml glass bottle. Insert two syringe needles into a rubber stopper (gauge 19), one from the bottom and the other from the top, to provide an inlet and an outlet line. Seal the glass bottle with this rubber stopper and attach a custom designed activated carbon filter to the inlet line (Figure 1A).</li>
 <li>The carbon filter is made using two 6 ml syringes. Cut the syringes in half and discard the plunger end. Fill each of the remaining pieces with cotton and activated charcoal before connecting them together using heat-shrink tubing.</li>
 <li>Connect the outlet line of the odor bottle (using polyethylene tubing, I.D. 0.86 mm) to the plastic tube (Nalgene FEP Tubing, I.D. 5.8 mm) that supplies a constant airflow across the antenna (Figure 1B).</li>
 <li>Direct carbon-filtered, dehumidified air (carrier gas; flow-rate, 0.75 L/min) towards the locust using a plastic tube placed within a few cm of the locust antenna.</li>
 <li>For odor stimulation, displace a constant volume (0.1 L/min) of the static headspace above the odor solution in the bottle. This is achieved by injecting an equal amount of dehumidified air into the bottle using a Pico-pump (WPI, PV-820). The vapors from the odor bottle are directed through the outlet line onto the airflow tube (Figure 1B).</li>
 <li>Remove the delivered odor vapors by placing a vacuum funnel ~10 cm behind the locust antenna.</li>
</ol>
2. Preparing Locust Antenna for Single Sensillum Recording
<ol>
 <li>Select a young-adult locust of either sex with fully-grown wings, but prior to the mating stage from a crowded colony. To restrain the locust, first amputate its legs. Seal the amputation sites with tissue adhesive (Vetbond, 3M). Secure the locust to a custom designed chamber using a small piece of electrical tape draped around its thorax (Figure 2A).</li>
 <li>Under a dissection microscope, make a shallow groove in the wax platform (Figure 2A) for antenna placement. Place the antenna into the groove and stabilize it using batik wax at the two ends of the antenna (Figure 2B; an electrowaxer is used for melting the wax).</li>
 <li>Insert a ground electrode (chlorided silver wire) into the thorax (~1 cm away from head). Use batik wax to both seal the incision site and hold the ground wire in place (Figure 2A).</li>
</ol>
3. Single Sensillum Recording to Monitor Odor-evoked Responses from Olfactory Receptor Neurons (ORNs)
<ol>
 <li>Place the stabilized locust antenna under a stereomicroscope (Leica M205C) on a vibration isolation table (TMC) (Figure 3A). Make sure that the base of the recording sensillum is clearly visible (Figure 3B).</li>
 <li>Use a micropipette puller (Sutter P-1000) to fabricate glass electrodes (impedance 3-10 M&Omega; when filled with locust saline9, tip diameter 1-3 &mu;m) using a borosilicate glass capillary tube (O.D. 1.2 mm, I.D. 0.69 mm).</li>
 <li>Place the glass electrode into a micropipette holder that is attached to a motorized micromanipulator (Sutter MP-285). Gently insert the electrode into the base of a sensillum (Figure 3B). Note that each sensillum can contain 3-50 ORNs in locusts10.</li>
 <li>Amplify the signal (10,000 times) using an A.C. amplifier (Grass P-55). Filter the signal between 0.3 - 10.0 kHz and acquire at a 15 kHz sampling rate (Figure 3D) using a data acquisition system (LabView, PCI-MIO-16E-4 DAQ cards; National Instruments).</li>
</ol>
4. Locust Dissection Procedure for Antennal Lobe and Mushroom Body Recordings
<ol>
 <li>Follow the restraining procedures as described in section 2.1. Position the locust in a custom-designed chamber as shown in Figure 4A and B.</li>
 <li>To perfuse saline during and after the dissection procedure, build a wax cup around the locust head. The wax cup should start just above the mouth parts, and extend beyond the compound eyes encompassing the region between the two antennae as shown in Figure 4C.</li>
 <li>To allow the antennae to pass through the wax cup, create small tunnels on both sides using plastic (polyethylene) tubing (5 mm long, I.D. 0.86 mm). Ensure that the plastic tubing can slide through the wax cup. The latter is achieved by using a rubber gasket that tightly wraps around the plastic tube but is attached to the wax-cup (Figure 4C).</li>
 <li>Using epoxy resin, attach the base of the antennae to the bottom end of the plastic tubing. This step ensures that the antennae are held in place even after the surrounding cuticle is removed.</li>
 <li>Keep the wax cup filled with saline solution9 from this point onwards. Begin by removing a central rectangular region between the two antennae (longer side of the rectangle aligned with the anteroposterior axis). Subsequently, remove cuticle in neighboring regions without disturbing the compound eyes and cuticle at the base of the antenna (Figure 4D).</li>
 <li>Using fine forceps, gently remove air sacs and fat bodies surrounding the brain. At the end of this step, the locust brain should be clearly seen (Figure 4D). Notice that the brain regions that process the olfactory information (with light yellow pigmentation) are situated between the two antennae.</li>
 <li>In locusts the gut runs below the brain and along the length of the body. To prevent movement of the gut from potentially destabilizing the preparation, gently pull the foregut and cut it using fine scissors. Make a small incision in the abdomen just above the rectum and remove the gut by pulling the hindgut with coarse forceps. To prevent a saline leak, tie the abdomen immediately anterior to the incision site with suture threads.</li>
 <li>Use a small platform made of a thin wire coated with a fine layer of wax to elevate the brain and stabilize it11 during electrophysiology as shown in Figure 4D.</li>
 <li>The insect brain is covered by a thin insulating sheath that needs to be removed prior to experiments. To desheath the brain, gently spread a small amount of an enzyme (0.3-0.4 mg protease, Sigma Aldrich) over the surface of the brain using fine forceps9. After ~5-10 s of enzyme application, rinse the brain thoroughly with saline. Using super-fine forceps very gently pinch and pull the sheath up and subsequently tear it open over the recording locations (AL and MB; shown in Figure 4E, F).</li>
</ol>
5. Multi-unit Recordings from the Antennal Lobe and the Mushroom Body
<ol>
 <li>Place the locust preparation (Figure 5A) under a stereomicroscope suspended from a boom stand placed on a vibration isolation table.</li>
 <li>Maintain a constant saline perfusion rate (about 0.04 L/hr) throughout the experiment. Use a chlorided silver wire immersed in the saline-filled wax cup as the ground electrode.</li>
 <li>For PN recordings, use a 16-channel silicon probe (NeuroNexus Technologies, item #A2x2-tet-3mm-150-150-121-A16, Figure 5B). Prior to each experiment, electroplate the electrodes with gold to achieve impedances in the 200-300 k&Omega; range. Use the circuit shown in Figure 7 for electroplating.</li>
 <li>Position the electrode close to the surface of the antennal lobe and gently insert it into the tissue using a manual micromanipulator (WPI, M3301R) (Figure 5D).</li>
 <li>Advance the electrode in ~10 &mu;m steps. Wait 2-3 min at each step and evaluate the acquired signal quality. In an ideal recording site, extracellular signals will be picked up by multiple recording channels and will have a high signal-to-noise ratio (SNR &gt; 3-5 times noise SDs).</li>
 <li>For KC recordings, use a custom-made twisted wire tetrode (Figure 5C; step-by-step fabrication procedure presented in Section 6). Electroplate these electrodes as discussed in step 5.3. Place the tetrode on the surface of the MB (Figure 5E) as KC somata are restricted to the superficial layer of the MB8.</li>
 <li>Both PN and KC recordings can be made simultaneously from same locust preparation as schematically shown in Figure 5A.</li>
 <li>Wait at least 15 min after finding the recording location to allow stabilization of the electrodes.</li>
 <li>Acquire all extracellular signals at 15 KHz, filter between 0.3-6 KHz, and amplify (10,000 times) using a 16 channel AC amplifier (Biology Electronics Shop; Caltech, Pasadena, CA) (Figure 6A, B).</li>
</ol>
6. Procedures to Make Twisted Wire Electrode for KC Recordings
<ol>
 <li>To design a multi-unit electrode for KC recordings, use insulated nickel chromium wire (RO800, 0.0005" filament)8.</li>
 <li>If eight electrodes are desired then wrap the wire around a 10-15 cm long piece of cardboard 4 times. The ends of the cardboard can be covered with plastic tubing to prevent the edges from cutting the wire. While wrapping, make sure there is little slack, but ensure that the wire is not taut. Handle the wire carefully as it breaks easily.</li>
 <li>Bunch the wires together at the top of the cardboard and use a piece of tape (Time Tape, T-534-RP) to hold them together. Cut the strands at the other end. Remove the wire strands and group the wires at the cut end as well using another piece of the tape.</li>
 <li>Using a titer plate shaker (Thermo Scientific, Model 4625Q), wind the strands together (~72 rev/min for 3 min) to form one twisted wire. The bottom of the taped strands can be clipped to the titer plate rotor and the top can be clipped to a boom stand. During winding, the wire should have little slack, yet not be taut.</li>
 <li>Melt the insulation together with a heat gun (Weller 6966C) to stick the individual strands together and form a single wire. 3-4 slow passes (3-4 sec each) over the length of the wire should be sufficient to heat and fuse the strands together. Then release the wire from the bottom and allow it to relax. Trim the wire near the ends to remove the tape.</li>
 <li>Insert one end of the wire through a 5-6 cm long, glass capillary tube (O.D. 1.0 mm, I.D. 0.58 mm). Tease apart the twisted wire at one end using fine tweezers and remove the coating by very briefly torching the end using a flame. This step must be done carefully; exposing the wire to the flame for too long will cause the strands to melt and tangle.</li>
 <li>Gently separate the 8 strands on the flamed end and solder each strand separately into an 8-pin IC socket. Coat the top of the IC socket with epoxy to hold the strands and glass capillary in place. Also place a small drop of epoxy at the other end of the capillary to hold the wire in place (Figure 5C).</li>
 <li>Finally, cut the tip of the wire at a 45 degree angle with carbide scissors about 0.5 cm from the capillary tip.</li>
</ol>

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No conflicts of interest declared.

Most sensory stimuli evoke combinatorial responses that are distributed across ensembles of neurons. Hence, simultaneous monitoring of multi-neuron activity is necessary to understand how stimulus-specific information is represented and processed by neural circuits in the brain. Here, we have demonstrated extracellular multi-unit recording techniques to characterize odor-evoked responses at the first three processing centers along the insect olfactory pathway. We note that the techniques presented here have been used in ...

<table border="1">
	<tbody>
		<tr>
			<td>Name</td>
			<td>Company</td>
			<td>Catalog Number</td>
			<td>Comments</td>
		</tr>
		<tr>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>Electrophysiology Equipment</td>
		</tr>
		<tr>
			<td>A.C. amplifier</td>
			<td>GRASS</td>
			<td>Model P55</td>
			<td>for single sensillum recordings</td>
		</tr>
		<tr>
			<td>Audio monitor (model 3300)</td>
			<td>A-M Systems</td>
			<td>940000</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Custom-made 16 channel pre-amplifier and amplifier</td>
			<td>Cal. Tech. Biology Electronics Shop</td>
			<td>&nbsp;</td>
			<td>for AL and MB recordings</td>
		</tr>
		<tr>
			<td>Data acquisition unit</td>
			<td>National Instruments</td>
			<td>BNC-2090</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Fiber optic light</td>
			<td>WPI</td>
			<td>SI-72-8</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Light source 115 V</td>
			<td>WPI</td>
			<td>NOVA</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Manual micromanipulator</td>
			<td>WPI</td>
			<td>M3301R</td>
			<td>for locust brain recordings</td>
		</tr>
		<tr>
			<td>Stereomicroscope1 on boom stand</td>
			<td>Leica</td>
			<td>M80</td>
			<td>for locust brain recordings</td>
		</tr>
		<tr>
			<td>Stereomicroscope2</td>
			<td>Leica</td>
			<td>M205C</td>
			<td>for single sensillum recordings</td>
		</tr>
		<tr>
			<td>Vibration-isolation table</td>
			<td>TMC</td>
			<td>63-500 series</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Motorized micromanipulator</td>
			<td>Sutter Instruments</td>
			<td>MP285/T</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Oscilloscope</td>
			<td>Tektronix</td>
			<td>TD2014B</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>Electrodes/Construction Tools</td>
		</tr>
		<tr>
			<td>16-channel electrode</td>
			<td>NeuroNexus</td>
			<td>A2x2-tet-3mm-150-121</td>
			<td>for antennal lobe recordings</td>
		</tr>
		<tr>
			<td>Borosilicate capillary tubes with filament, ID 0.69 mm</td>
			<td>Sutter Instruments</td>
			<td>BF120-69-10</td>
			<td>for making glass electrodes</td>
		</tr>
		<tr>
			<td>Micropipette puller</td>
			<td>Sutter Instruments</td>
			<td>P-1000</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Function generator</td>
			<td>Multimeter Warehouse</td>
			<td>SG1639A</td>
			<td>for gold-plating electrodes</td>
		</tr>
		<tr>
			<td>Gold plating solution (non cyanide)</td>
			<td>SIFCO Industries</td>
			<td>NC SPS 5355</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Impedance tester</td>
			<td>BAK Electronics Inc.</td>
			<td>IMP-2</td>
			<td>for gold-plating electrodes</td>
		</tr>
		<tr>
			<td>Switch rotary</td>
			<td>Electroswitch</td>
			<td>C7D0123N</td>
			<td>for gold-plating electrodes</td>
		</tr>
		<tr>
			<td>Pulse isolator</td>
			<td>WPI</td>
			<td>A365</td>
			<td>for gold-plating electrodes</td>
		</tr>
		<tr>
			<td>Q series electrode holder</td>
			<td>Warner Instruments</td>
			<td>64-1091</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Silver wire 0.010" diameter</td>
			<td>A-M Systems</td>
			<td>782500</td>
			<td>ground electrode</td>
		</tr>
		<tr>
			<td>8 pin DIP IC socket</td>
			<td>Digikey</td>
			<td>ED90032-ND</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Borosilicate capillary tubes with filament, ID 0.58 mm</td>
			<td>Warner Instruments</td>
			<td>64-0787</td>
			<td>twisted wire tetrode construction</td>
		</tr>
		<tr>
			<td>Heat gun</td>
			<td>Weller</td>
			<td>6966C</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Rediohm-800 wire</td>
			<td>Kanthal Precision Technologies</td>
			<td>PF002005</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Titer plate shaker</td>
			<td>Thermo Scientific</td>
			<td>4625Q</td>
			<td>twisting wires</td>
		</tr>
		<tr>
			<td>Carbide scissors, 4.5"</td>
			<td>Biomedical Research Instr</td>
			<td>25-1000</td>
			<td>for cutting twisted tetrode wires</td>
		</tr>
		<tr>
			<td>Fine point tweezers</td>
			<td>HECO</td>
			<td>91-EF5-SA</td>
			<td>for teasing tetrode wires apart</td>
		</tr>
		<tr>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>Odor Delivery</td>
		</tr>
		<tr>
			<td>6 ml syringe</td>
			<td>Kendall</td>
			<td>1180600777</td>
			<td>for custom designed activated carbon filter</td>
		</tr>
		<tr>
			<td>Brown odor bottles</td>
			<td>Fisher</td>
			<td>08-912-165</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Charcoal</td>
			<td><a href="http://BuyActivatedCharcoal.com" target="_blank">BuyActivatedCharcoal.com</a></td>
			<td>GAC-48C</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Desiccant</td>
			<td>Drierite</td>
			<td>23005</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Drierite gas drying jar</td>
			<td>Fischer Scientific</td>
			<td>09-204</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Heat shrink tubing</td>
			<td>3M</td>
			<td>EPS-200</td>
			<td>odor filter preparation</td>
		</tr>
		<tr>
			<td>Hypodermic needle aluminum hub, gauge 19</td>
			<td>Kendall</td>
			<td>8881-200136</td>
			<td>for providing inlet and outlet lines for odor bottles</td>
		</tr>
		<tr>
			<td>Mineral oil</td>
			<td>Mallinckrodt Chemicals</td>
			<td>6357-04</td>
			<td>for odor dilution</td>
		</tr>
		<tr>
			<td>Nalgene plastic tubing, 890 FEP</td>
			<td>Thermo Scientific</td>
			<td>8050-0310</td>
			<td>for carrier gas delivery</td>
		</tr>
		<tr>
			<td>Pneumatic picopump</td>
			<td>WPI</td>
			<td>sys-pv820</td>
			<td>for odor delivery</td>
		</tr>
		<tr>
			<td>Polyethylene tubing ID 0.86 mm</td>
			<td>Intramedic</td>
			<td>427421</td>
			<td>for odor bottle outlet connections and saline profusion tubing</td>
		</tr>
		<tr>
			<td>Stoppers</td>
			<td>Lab Pure</td>
			<td>97041</td>
			<td>for sealing odor bottles</td>
		</tr>
		<tr>
			<td>Time tape</td>
			<td>PDC</td>
			<td>T-534-RP</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Tubing luer</td>
			<td>Cole-Parmer</td>
			<td>30600-66</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Vacuum tube</td>
			<td>McMaster-Carr</td>
			<td>5488K66</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>Preparation/Dissection</td>
		</tr>
		<tr>
			<td>100 x 15 mm petri dish</td>
			<td>VWR International</td>
			<td>89000-304</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>18 AWG copper stranded wire</td>
			<td>Lapp Kabel</td>
			<td>4510013</td>
			<td>wire insulation is used as rubber gaskets</td>
		</tr>
		<tr>
			<td>22 AWG stranded hookup wire</td>
			<td>AlphaWire</td>
			<td>1551</td>
			<td>brain platform</td>
		</tr>
		<tr>
			<td>Batik wax</td>
			<td>Jacquard</td>
			<td>7946000</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Dental periphery Wax</td>
			<td>Henry-Schein Dental</td>
			<td>6652151</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Electrowaxer</td>
			<td>Almore International</td>
			<td>66000</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Epoxy, 5 min</td>
			<td>Permatex</td>
			<td>84101</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Hypodermic needle aluminum hub</td>
			<td>Kendall</td>
			<td>8881-200136</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Protease from Streptomyces griseus</td>
			<td>Sigma-Aldrich</td>
			<td>P5147</td>
			<td>for desheathing locust brain</td>
		</tr>
		<tr>
			<td>Suture thread non-sterile</td>
			<td>Fisher</td>
			<td>NC9087024</td>
			<td>for tying the abdomen after gut removal</td>
		</tr>
		<tr>
			<td>Vetbond</td>
			<td>3M</td>
			<td>1469SB</td>
			<td>for sealing amputation sites</td>
		</tr>
		<tr>
			<td>Dumont #1 forceps (coarse)</td>
			<td>WPI</td>
			<td>500335</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Dumont #5 titanium forceps (fine)</td>
			<td>WPI</td>
			<td>14096</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Dumont #5SF forceps (super-fine)</td>
			<td>WPI</td>
			<td>500085</td>
			<td>desheathing locust brain</td>
		</tr>
		<tr>
			<td>10 cm dissecting scissors</td>
			<td>WPI</td>
			<td>14393</td>
			<td>for removing legs and wings</td>
		</tr>
		<tr>
			<td>Vannas scissors (fine)</td>
			<td>WPI</td>
			<td>500086</td>
			<td>for removing cuticle, cutting the foregut</td>
		</tr>
		<tr>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>Saline Profusion</td>
		</tr>
		<tr>
			<td>Extension set with rate flow regulator</td>
			<td>Moore Medical</td>
			<td>69136</td>
			<td>for regulating saline flow</td>
		</tr>
		<tr>
			<td>IV administration set with Y injection site</td>
			<td>Moore Medical</td>
			<td>73190</td>
			<td>for regulating saline flow</td>
		</tr>
	</tbody>
</table>

multi-unit recording methods to characterize neural activity in the locust (schistocerca americana) olfactory circuits

Detection and interpretation of olfactory cues are critical for the survival of many organisms. Remarkably, species across phyla have strikingly similar olfactory systems suggesting that the biological approach to chemical sensing has been optimized over evolutionary time1. In the insect olfactory system, odorants are transduced by olfactory receptor neurons (ORN) in the antenna, which convert chemical stimuli into trains of action potentials. Sensory input from the ORNs is then relayed to the antennal lobe (AL; a structure analogous to the vertebrate olfactory bulb). In the AL, neural representations for odors take the form of spatiotemporal firing patterns distributed across ensembles of principal neurons (PNs; also referred to as projection neurons)2,3. The AL output is subsequently processed by Kenyon cells (KCs) in the downstream mushroom body (MB), a structure associated with olfactory memory and learning4,5. Here, we present electrophysiological recording techniques to monitor odor-evoked neural responses in these olfactory circuits.
First, we present a single sensillum recording method to study odor-evoked responses at the level of populations of ORNs6,7. We discuss the use of saline filled sharpened glass pipettes as electrodes to extracellularly monitor ORN responses. Next, we present a method to extracellularly monitor PN responses using a commercial 16-channel electrode3. A similar approach using a custom-made 8-channel twisted wire tetrode is demonstrated for Kenyon cell recordings8. We provide details of our experimental setup and present representative recording traces for each of these techniques.

Odor-evoked responses of a single ORN to two different alcohols are shown in the Figure 3D. Depending on the recording location (sensilla type, placement of the electrode) multi-unit recordings can be achieved.
A raw extracellular waveform from an AL recording is shown in Figure 6A. Action potentials or spikes of varying amplitudes originating from different PNs can be observed in this voltage trace. Although the locust antennal lobe has excitatory projection ...

Watch this Scientific Journal Video about Multi-unit Recording Methods to Characterize Neural Activity in the Locust Schistocerca Americana Olfactory Circuits at JoVE.com

Multi-unit Recording Methods to Characterize Neural Activity in the Locust Schistocerca Americana Olfactory Circuits

We demonstrate variations of the extracellular multi-unit recording technique to characterize odor-evoked responses in the first three stages of the invertebrate olfactory pathway. These techniques can easily be adapted to examine ensemble activity in other neural systems as well.

Multi-unit Recording Methods to Characterize Neural Activity in the Locust (Schistocerca Americana) Olfactory Circuits

Detection and interpretation of olfactory cues are critical for the survival  of many organisms. Remarkably, species across phyla have strikingly similar ...

multi-unit-recording-methods-to-characterize-neural-activity-locust

Research

JoVE Journal

Neuroscience

26.8K Views.  Washington University in St. Louis. We demonstrate variations of the extracellular multi-unit recording technique to characterize odor-evoked responses in the first three stages of the invertebrate olfactory pathway. These techniques can easily be adapted to examine ensemble activity in other neural systems as well.