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Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy

78.0K Views

10:00 min

March 24th, 2014

DOI :

10.3791/51100-v

March 24th, 2014


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Keywords Immunological Synapse

Rozdziały w tym wideo

0:05

Title

1:25

Activation of NK Cell on Antibody-coated Coverslips

3:19

Fixing, Permeabilizing, and Staining NK Cells on Coverslips

4:52

Mounting the Coverslips

5:35

STED Imaging

8:21

Optimizing STED Imaging: F-actin in NK Cells

9:30

Conclusion

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