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Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

16.5K Views

09:02 min

January 8th, 2015

DOI :

10.3791/52155-v

January 8th, 2015


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Keywords Subcloning

Rozdziały w tym wideo

0:05

Title

1:04

Multiplex Recombineering Oligos

2:00

BAC Clone Transformation

3:56

Insertion Cassettes and Subcloning Plasmids

5:03

Subcloning Plus Insertion

7:07

Analysis of Recombinants

7:48

Results: Subcloning Plus Insertion (SPI)

8:39

Conclusion

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