NOTE: The following protocol follows our internal guidelines and standard operation procedures approved by the chair of our department. The same protocol was approved by our institutional review board and was used for studies published in a previous paper8.
1. Pre-ultrasound Scan Preparation
<ol>
	<li>Place the patient in a supine position with an optional elevated head of bed or stretcher for comfort if the patient is awake.</li>
	<li>Position the ultrasound machine in a line with operator&#180;s position and the puncture site to prevent operator&#180;s head or body twisting to see the display. Turn on the ultrasound machine and connect the micro-convex ultrasound probe.</li>
	<li>Choose a pre-set that allows deep tissue penetration, e.g., set probe to 8 MHz or lower. Use alcoholic disinfection on skin for acoustic coupling of the ultrasound probe.</li>
</ol>
2. Ultrasound Scan of the CVC Insertion Site and Superior Vena Cava
<ol>
	<li>Place the micro-convex ultrasound probe on the puncture site. 
	NOTE: A B(rightness)-mode short axis view and out of plane puncture technique is preferred. Adjust gain, depth and focus position for an optimal image.</li>
	<li>Confirm vein by applying pressure to test for compressibility and to exclude thrombus at puncture site. Use color flow doppler if there is uncertainty. Move ultrasound probe centrally to detect potential thrombus.</li>
</ol>
3. Supraclavicular Fossa Ultrasound View
<ol>
	<li>Place the ultrasound probe in the right supraclavicular fossa to obtain a view of the confluence of the internal jugular vein and subclavian vein to detect a potential thrombus. Change gain, depth and focus using knobs of the ultrasound machine. 
	NOTE: Set gain high enough to differentiate between hyperechoic vessel walls and hypoechoic vessel lumen. Set depth (10-12 cm) to have a full view of the SVC and right pulmonary artery (RPA). Set the Focus to the level of the RPA.</li>
	<li>Increase depth (4-6 cm) using the knob of the ultrasound machine to obtain a view of the confluence of the brachiocephalic veins. This will also correspond to the beginning of the upper superior vena cava. Adjust depth (10-12 cm) and angulation of the ultrasound probe to obtain a full longitudinal view of the superior vena cava, adjacent ascending aorta and right pulmonary artery. 
	NOTE: Probe frequency will be adjusted by the ultrasound machine depending on depth of imaging.</li>
	<li>Evaluate the brachiocephalic veins and the superior vena cava for intravenous thrombus. Turn the ultrasound probe 90&#176; clockwise to obtain a sagittal view of the superior vena cava. Observe the right pulmonary artery in short axis dorsal of the lower superior vena cava. Additionally, observe a short axis view of the pulmonary veins distal of the right pulmonary artery. Use optional Doppler to confirm superior vena cava.</li>
</ol>
4. Ultrasound Guided Venipuncture
<ol>
	<li>Prepare CVC kit and dress with sterile gown and gloves. Disinfect skin with alcohol wipes and apply sterile drapes. Insert ultrasound probe into a sterile cover. Ultrasound guided venipuncture can be performed in non-sedated as well as in anesthetized patients. 
	NOTE: For non-sedated patients a local infiltration anesthesia before venipuncture is highly recommended.</li>
	<li>Obtain an ultrasound view of the vein to be punctured. Right-handed operators use the needle attached to a syringe in the right hand and the ultrasound probe in the left hand.</li>
	<li>Perform a venipuncture under ultrasound guidance. 
	NOTE: Out of plane and in plane techniques are both appropriate.</li>
	<li>Following venipuncture, insert the guidewire through the needle. At this point, a long axis view of the vein can confirm the guidewire within the vein.</li>
</ol>
5. Ultrasound Guided Guidewire Advancement
<ol>
	<li>Switch ultrasound probe to the right hand. Obtain a view of the superior vena cava by adjusting depth and focus position of the ultrasound image (Figure 3A and supplemental video loop). 
	NOTE: This has to be done by an assisting person or by the operator himself if the ultrasound machine is operated with a sterile cover. Angulation of the ultrasound probe needs to be in a small angle in relation to neck.</li>
	<li>Advance the guidewire with the left hand. Do not introduce the guidewire too far into the superior vena cava because this will make it difficult to identify the guidewire tip. Visualize the guidewire J-tip during advancement and crossing of the upper wall of the right pulmonary artery (Figure 3B).</li>
	<li>If the ultrasound beam cannot be aligned with the J-tip and the right pulmonary artery, use the ascending aorta as a landmark. Turn the ultrasound probe 90&#176; clockwise to obtain a sagittal view of the superior vena cava. With this view, confirm the guidewire position relative to the right pulmonary artery observed in the short axis.</li>
</ol>
6. Measurement of Insertion Depth
<ol>
	<li>Use the central venous catheter to measure the insertion depth of the guidewire. In order to do so, align the 20 cm markings of the CVC and the guidewire and read the cm marking at the venipuncture site. 
	NOTE: The guidewire has markings at 10, 20 cm and at the position indicating that the J-tip leaves the distal lumen. These markings may be different depending on the manufacturer. Be careful not to move the guidewire. 
	NOTE: From this point on, complete placement according to standard hospital protocol, including dilation of skin and vein, insertion of the CVC over guidewire, aspiration and flushing and suture.</li>
</ol>
7. Lung Ultrasound
<ol>
	<li>In the perioperative setting, perform a lung ultrasound examination in the patient to exclude pneumothorax following CVC placement. 
	NOTE: This procedure has been described by Lichtenstein et al.11.</li>
	<li>Place the ultrasound probe in all four quadrants of the anterior chest bilaterally to obtain view in B-mode for lung sliding and M-mode for seashore sign. Preferably use a linear ultrasound probe. For obese patients, use an ultrasound probe with higher penetration, e.g., curved array or micro-convex probes.</li>
</ol>
8. Central Venous Catheter Change Over Guidewire
NOTE: CVC change over guidewire is a single contributor to catheter-related blood stream infection and should be avoided. In patients with a high risk of mechanical complication related to CVC insertion or limited venous access, CVC change over guidewire still can be considered12.
<ol>
	<li>In case of a misplaced central venous catheter (not using the ultrasound guided tip position method), obtain a supraclavicular view to advance the guidewire into the correct position. Under sterile conditions, introduce a guidewire into the distal lumen of the misplaced central venous catheter. Change of a CVC over a guidewire with ultrasound guidance works in non-sedated patients as well as in anesthetized patients.
	<ol>
		<li>Pull back the central venous catheter. Visualize the guidewire from thesupraclavicular fossa view.</li>
		<li>Advance the guidewire into the correct position in the superior vena cava. Introduce the new central venous catheter over the guidewire.</li>
		<li>From this point on, complete procedure according to standard hospital protocol.</li>
	</ol>
	</li>
</ol>

<ol>
	<li>Troianos, C. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Special+articles:+guidelines+for+performing+ultrasound+guided+vascular+cannulation:+recommendations+of+the+American+Society+of+Echocardiography+and+the+Society+Of+Cardiovascular+Anesthesiologists.">Special articles: guidelines for performing ultrasound guided vascular cannulation: recommendations of the American Society of Echocardiography and the Society Of Cardiovascular Anesthesiologists.</a> Anesth Analg. 114 (1), 46-72 (2012).</li><li>Cavanna, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultrasound-guided+central+venous+catheterization+in+cancer+patients+improves+the+success+rate+of+cannulation+and+reduces+mechanical+complications:+a+prospective+observational+study+of+1,978+consecutive+catheterizations.">Ultrasound-guided central venous catheterization in cancer patients improves the success rate of cannulation and reduces mechanical complications: a prospective observational study of 1,978 consecutive catheterizations.</a> World J Surg Oncol. 8 (91), (2010).</li><li>Wirsing, M., Schummer, C., Neumann, R., Steenbeck, J., Schmidt, P., Schummer, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Is+traditional+reading+of+the+bedside+chest+radiograph+appropriate+to+detect+intraatrial+central+venous+catheter+position.">Is traditional reading of the bedside chest radiograph appropriate to detect intraatrial central venous catheter position.</a> Chest. 134 (3), 527-533 (2008).</li><li>Ender, J., Erdoes, G., Krohmer, E., Olthoff, D., Mukherjee, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transesophageal+echocardiography+for+verification+of+the+position+of+the+electrocardiographically-placed+central+venous+catheter.">Transesophageal echocardiography for verification of the position of the electrocardiographically-placed central venous catheter.</a> J Cardiothorac Vasc Anesth. 23 (4), 457-461 (2009).</li><li>Pittiruti, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+intracavitary+ECG+method+for+positioning+the+tip+of+central+venous+catheters:+results+of+an+Italian+multicenter+study.">The intracavitary ECG method for positioning the tip of central venous catheters: results of an Italian multicenter study.</a> J Vasc Access. 13 (3), 357-365 (2012).</li><li>Matsushima, K., Frankel, H. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bedside+ultrasound+can+safely+eliminate+the+need+for+chest+radiographs+after+central+venous+catheter+placement:+CVC+sono+in+the+surgical+ICU+(SICU).">Bedside ultrasound can safely eliminate the need for chest radiographs after central venous catheter placement: CVC sono in the surgical ICU (SICU).</a> J Surg Res. 163 (1), 155-161 (2010).</li><li>Matsushima, K., Frankel, H. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Detection+of+central+venous+catheter+insertion-related+complication+using+bedside+ultrasound:+the+CVC+sono.">Detection of central venous catheter insertion-related complication using bedside ultrasound: the CVC sono.</a> J Trauma. 70 (6), 1561-1563 (2011).</li><li>Kim, S. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultrasound+confirmation+of+central+venous+catheter+position+via+a+right+supraclavicular+fossa+view+using+a+microconvex+probe:+A+observational+pilot+study.">Ultrasound confirmation of central venous catheter position via a right supraclavicular fossa view using a microconvex probe: A observational pilot study.</a> Eur J Anaesthesiol. , (2013).</li><li>Lanspa, M. J., Fair, J., Hirshberg, E. L., Grissom, C. K., Brown, S. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultrasound-guided+Subclavian+Vein+Cannulation+Using+a+Micro-Convex+Ultrasound+Probe.">Ultrasound-guided Subclavian Vein Cannulation Using a Micro-Convex Ultrasound Probe.</a> Ann Am Thorac Soc. 11 (4), 583-586 (2014).</li><li>Weber, S. U., Kim, S. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+right+supraclavicular+ultrasound+view+for+real-time+ultrasound-guided+definite+placement+of+a+central+venous+catheter+with+a+microconvex+transducer.">The right supraclavicular ultrasound view for real-time ultrasound-guided definite placement of a central venous catheter with a microconvex transducer.</a> Eur J Anaesthesiol. 31 (3), 173-174 (2014).</li><li>Lichtenstein, D. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultrasound+diagnosis+of+occult+pneumothorax.">Ultrasound diagnosis of occult pneumothorax.</a> Crit Care Med. 33 (6), 1231-1238 (2005).</li><li>Garnacho-Montero, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Risk+factors+and+prognosis+of+catheter-related+bloodstream+infection+in+critically+ill+patients:+a+multicenter+study.">Risk factors and prognosis of catheter-related bloodstream infection in critically ill patients: a multicenter study.</a> Intensive Care Med. 34 (12), 2185-2193 (2008).</li><li>Schummer, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intra-atrial+ECG+is+not+a+reliable+method+for+positioning+left+internal+jugular+vein+catheters.">Intra-atrial ECG is not a reliable method for positioning left internal jugular vein catheters.</a> Br J Anaesth. 91 (4), 481-486 (2003).</li></ol>

The authors declare that they have no competing financial interests.

This procedure allows for visualization of ultrasound guided venipuncture and central venous catheter tip position in real time via the right supraclaviculare fossa view. In combination with a lung ultrasound scan, this approach could replace chest radiograph for confirmation of catheter tip position and exclusion of a pneumothorax.
It is critical for ultrasound trained operators to become familiar with the ultrasound anatomy provided by the supraclavicular fossa view and correctly identify th...

Central venous catheter (CVC) tip position has to be confirmed since misplaced catheters can lead to trauma and erosion of endothelium and vessel walls with subsequent potentially fatal pericardial tamponade1. Furthermore, CVC in the upper superior vena cava (SVC) can provoke thrombotic events, especially in cancer patients. The same is true for misplaced CVC in opposite brachiocephalic vein or internal jugular vein (IJV)2.
Several methods have been established to confirm CVC tip position in the lower SVC. Chest radiograph has been performed traditionally as a bedside method to visualize the CVC tip and exclude pneumothorax. However, additionally to exposure to radiation and costs, the accuracy of chest radiograph for tip position and sensitivity for pneumothorax have been questioned, specifically for the patient in a supine position3. Computer tomography is highly accurate and sensitive for both CVC tip position and pneumothorax, respectively. However, the costs and exposure to radiation do not justify this method for the sole indication of CVC tip confirmation. Transesophageal echocardiography (TEE) is highly accurate for tip position, but indication for TEE is limited to patients with cardiac pathology4. Intra-cardiac electrocardiography (ECG) is an accurate method but depends on a p-wave cardiac rhythm5.
Parasternal and subcostal ultrasound view were suggested to verify the CVC tip position, but success depends on deep insertion of the guidewire into the right atrium. A negative result does not exclude misplaced catheters6,7. The right supraclavicular fossa ultrasound view has been solely described for diagnostic purposes in cardiac failure. In a pilot study, we have demonstrated that this view can also be safely used for venipuncture of the right IJV and accurate positioning of the CVC guidewire with subsequent CVC placement in real-time8. The right supraclavicular fossa ultrasound view can be established with a microconvex probe which has the advantage of a small footprint, deep pentetration and, yet a sufficient resolution for venipuncture when compared to a linear probe8-10.
The proposed procedure has the advantage of visualizing venipuncture and guidewire advancement with a single ultrasound probe within the sterile field in real-time. CVCs insertion length does not have to be corrected afterwards and extra manipulations and potential sources of contamination are prevented. In conjunction with lung ultrasound for exclusion of a pneumothorax, the proposed procedure has the potential to replace chest radiograph for CVC tip position confirmation and exclusion of pneumothorax, reducing costs and exposure to radiation and increasing patient comfort in any hospital setting where an ultrasound machine equipped with a microconvex ultrasound probe is available.

<table border="0" cellpadding="0" cellspacing="0" width="1058">
	<tbody>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Name of Material/ Equipment</td>
			<td style="width:93px;">Company</td>
			<td style="width:119px;">Catalog Number</td>
			<td style="width:631px;">Comments/Description</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">skin desinfection</td>
			<td style="width:93px;">Ecolab</td>
			<td align="right" style="width:119px;">2686556</td>
			<td>Skinsept G</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;">8C-RS microconvex probe</td>
			<td style="width:93px;">GE Healthcare</td>
			<td style="width:119px;">8C-RS</td>
			<td>Any ultrasound machine equipped with a microconvex ultrasound probe should be suitable for this method</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Logiq e ultrasound machine</td>
			<td style="width:93px;">GE Healthcare</td>
			<td style="width:119px;">Logiq e</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">CVC Kit</td>
			<td style="width:93px;">Teleflex Medical</td>
			<td style="width:119px;">OW-15802-E</td>
			<td>8 Fr. 2 Lumen Blue-Flex tip catheter</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Sterile gloves</td>
			<td style="width:93px;">Protexix</td>
			<td style="width:119px;">2D72NT70X</td>
			<td>LatexMicro</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Sterile gown</td>
			<td style="width:93px;">Medline</td>
			<td style="width:119px;">OP9512CE</td>
			<td>OPS Advanced</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">sterile ultrasound probe cover</td>
			<td style="width:93px;">CIVCO</td>
			<td style="width:119px;">610-637</td>
			<td>CIV-Fle Transducer Cover; sterile 8.9 x 91.5 cm</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">suture</td>
			<td style="width:93px;">Ethicon</td>
			<td style="width:119px;">EH7723</td>
			<td>Ethibond</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">12L-RS linear ultrasound probe</td>
			<td style="width:93px;">GE Healthcare</td>
			<td style="width:119px;"></td>
			<td>any linear ultrasound probe is suitable for lung ultrasound</td>
		</tr>
	</tbody>
</table>

the supraclavicular fossa ultrasound view for central venous catheter placement and catheter change over guidewire

The supraclavicular fossa ultrasound view can be useful for central venous catheter (CVC) placement. Venipuncture of the internal jugular veins (IJV) or subclavian veins is performed with a micro-convex ultrasound probe, using a neonatal abdominal preset with a probe frequency of 10 Mhz at a depth of 10-12 cm. Following insertion of the guidewire into the vein, the probe is shifted to the right supraclavicular fossa to obtain a view of the superior vena cava (SVC), right pulmonary artery and ascending aorta. Under real-time ultrasound view, the guidewire and its J-tip is visualized and pushed forward to the lower SVC. Insertion depth is read from guidewire marks using central venous catheter. CVC is then inserted following skin and venous dilation. The supraclavicular fossa view is most suitable for right IJV CVC insertion. If other insertion sites are chosen the right supraclavicular fossa should be within the sterile field. Scanning of the IJVs, brachiocephalic veins and SVC can reveal significant thrombosis before venipuncture. Misplaced CVCs can be corrected with a change over guidewire technique under real-time ultrasound guidance. In conjunction with a diagnostic lung ultrasound scan, this technique has a potential to replace chest radiograph for confirmation of CVC tip position and exclusion of pneumothorax. Moreover, this view is of advantage in patients with a non-p-wave cardiac rhythm were an intra-cardiac electrocardiography (ECG) is not feasible for CVC tip position confirmation. Limitations of the method are lack of availability of a micro-convex probe and the need for training.

In a previously published study, chest radiographs were obtained in all patients following ultrasound guided CVC placement8. The CVC tip position in relation to the carina on chest radiographs was analysed by a radiologist who confirmed a correct position of the CVC in the lower SVC in all investigated patients. In 90% of all cases CVC tip was within 35 mm distance of the carina. In 17% the CVC tip was above, in 19% at the level of and in 64% below the carina. In 4% the CVC tip was more than 55 mm below the ca...

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The Supraclavicular Fossa Ultrasound View for Central Venous Catheter Placement and Catheter Change Over Guidewire

The overall goal is to enable clinicians to insert a central venous catheter under real-time ultrasound guidance via the right supraclavicular fossa view of the lower part of the superior vena cava. This view is useful for different catheter insertion sites, thrombosis detection before insertion and ultrasound guided correction of misplaced catheters.

The supraclavicular fossa ultrasound view can be useful for central venous catheter (CVC) placement. Venipuncture of the internal jugular veins (IJV) or ...

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18.7K Views.  University Hospital Bonn. The overall goal is to enable clinicians to insert a central venous catheter under real-time ultrasound guidance via the right supraclavicular fossa view of the lower part of the superior vena cava. This view is useful for different catheter insertion sites, thrombosis detection before insertion and ultrasound guided correction of misplaced catheters.