Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke

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11.1K Views

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09:50 min

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February 12th, 2015

DOI :

10.3791/52325-v

February 12th, 2015

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Diana Kuehn¹, Shoaib Majeed¹, Emmanuel Guedj¹, Remi Dulize¹, Karine Baumer¹, Anita Iskandar¹, Stephanie Boue¹, Florian Martin¹, Radina Kostadinova¹, Carole Mathis¹, Nikolai V. Ivanov¹, Stefan Frentzel¹, Julia Hoeng¹, Manuel C. Peitsch¹

¹Biological Systems Research, Philip Morris International R&D, Philip Morris Products S.A.

Podsumowanie

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Keywords Organotypic 3D Tissue Culture

Bronchial Tissue

Nasal Tissue

Cigarette Smoke Exposure

Impact Assessment

Ciliary Beating Frequency

Cytochrome P450 Activity

Gene Expression

Systems Biology

Xenobiotic Metabolism

Rozdziały w tym wideo

0:05

Title

1:28

Culture of Organotypic Bronchial Tissue

2:04

In vitro Cigarette Smoke (CS) Exposure

3:22

Measurement of Ciliary Beating & Transepithelial Electrical Resistance (TEER)

4:20

Cytochrome P450 (CYP) 1A1/1B1 Activity

5:02

RNA Extraction

6:35

Microarray Workflow & Washing and Staining

8:07

Results: Applicability of Human Organotypic Tissue Models

9:03

Conclusion

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