Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium
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08:46 min
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September 1st, 2015
DOI :
September 1st, 2015
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0:05
Title
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Isolation of Renal Cysts and Connecting Tubules/Collecting Duct Segments
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Single Channel Patch-clamp Electrophysiology
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Ratiometric Epifluorescence Measurement of Intracellular Calcium Concentration in the Epithelial Cells
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Results: Effect of Benzamil Treatment and ATP in the Cystic Cells of PCK Rats
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Conclusion
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