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Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

9.7K Views

08:46 min

September 1st, 2015

DOI :

10.3791/53035-v

September 1st, 2015


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Keywords Patch Clamp

Chapters in this video

0:05

Title

2:14

Isolation of Renal Cysts and Connecting Tubules/Collecting Duct Segments

5:02

Single Channel Patch-clamp Electrophysiology

5:32

Ratiometric Epifluorescence Measurement of Intracellular Calcium Concentration in the Epithelial Cells

6:55

Results: Effect of Benzamil Treatment and ATP in the Cystic Cells of PCK Rats

8:08

Conclusion

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